Immobilization of bacterial proteases on water-solved polymer by means of electron beam

Immobilization of bacterial proteases on water-solved polymer by means of electron beam

Possibility of electron beam usage for proteases' immobilization on 1,4-polyalkylene oxide (1,4-PAO) was studied to obtain biologically active complex for multi-purpose usage. It is shown that immobilization of Bacillus Subtilis protease takes place due to free-radical linking of enzyme and car...

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Veröffentlicht in:Radiation physics and chemistry (Oxford, England : 1993) England : 1993), 1996, Vol.48 (6), p.795-797
Hauptverfasser: Gonchar, A.M., Auslender, V.L.
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creator Gonchar, A.M.
Auslender, V.L.
description Possibility of electron beam usage for proteases' immobilization on 1,4-polyalkylene oxide (1,4-PAO) was studied to obtain biologically active complex for multi-purpose usage. It is shown that immobilization of Bacillus Subtilis protease takes place due to free-radical linking of enzyme and carrier with formation of mycellium-like structures. Immobilization improves heat resistance of enzyme up to 60°C without substrate and up to 80°C in presence of substrate, widens range of pH activity in comparison with non-immobilized forms. Immobilized proteases do not contain peroxides or long-live radicals. Our results permitted to create technologies for production of medical and veterinary preparations, active components for wool washing agents and leather fabrication technology.
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title Immobilization of bacterial proteases on water-solved polymer by means of electron beam
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