An in situ method for determining bacterial survival on food preparation surfaces using a redox dye
A simple method is described for the direct enumeration of viable bacteria dried on test surfaces. Inoculated surfaces were overlayed with agar and after incubation nitroblue tetrazolium solution (pale yellow) was used to stain colonies (purple) at the agar‐test surface interface. Stained colonies c...
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| Veröffentlicht in: | Letters in applied microbiology 1996-11, Vol.23 (5), p.325-328 |
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| container_end_page | 328 |
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| container_issue | 5 |
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| container_title | Letters in applied microbiology |
| container_volume | 23 |
| creator | Barnes, B.I. Cassar, C.A. Halablab, M.A. Parkinson, N.H. Miles, R.J. |
| description | A simple method is described for the direct enumeration of viable bacteria dried on test surfaces. Inoculated surfaces were overlayed with agar and after incubation nitroblue tetrazolium solution (pale yellow) was used to stain colonies (purple) at the agar‐test surface interface. Stained colonies could be readily detected and counted even against the opaque background of ceramic tile or stainless steel or when present within opaque films of milk or serum. Recovery of bacteria by this method was approximately fivefold greater than using a conventional swabbing procedure. The method was used to demonstrate the marked effect of the composition of the suspension fluid, in which bacteria were dried, and the length of surface exposure upon bacterial survival. |
| doi_str_mv | 10.1111/j.1472-765X.1996.tb00200.x |
| format | Article |
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Inoculated surfaces were overlayed with agar and after incubation nitroblue tetrazolium solution (pale yellow) was used to stain colonies (purple) at the agar‐test surface interface. Stained colonies could be readily detected and counted even against the opaque background of ceramic tile or stainless steel or when present within opaque films of milk or serum. Recovery of bacteria by this method was approximately fivefold greater than using a conventional swabbing procedure. 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Inoculated surfaces were overlayed with agar and after incubation nitroblue tetrazolium solution (pale yellow) was used to stain colonies (purple) at the agar‐test surface interface. Stained colonies could be readily detected and counted even against the opaque background of ceramic tile or stainless steel or when present within opaque films of milk or serum. Recovery of bacteria by this method was approximately fivefold greater than using a conventional swabbing procedure. The method was used to demonstrate the marked effect of the composition of the suspension fluid, in which bacteria were dried, and the length of surface exposure upon bacterial survival.</description><subject>Bacteria - isolation & purification</subject><subject>Bacterial Physiological Phenomena</subject><subject>Biological and medical sciences</subject><subject>Escherichia coli</subject><subject>Food industries</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. 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| fulltext | fulltext |
| identifier | ISSN: 0266-8254 |
| ispartof | Letters in applied microbiology, 1996-11, Vol.23 (5), p.325-328 |
| issn | 0266-8254 1472-765X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15909601 |
| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Bacteria - isolation & purification Bacterial Physiological Phenomena Biological and medical sciences Escherichia coli Food industries Food Microbiology Fundamental and applied biological sciences. Psychology Tetrazolium Salts - metabolism |
| title | An in situ method for determining bacterial survival on food preparation surfaces using a redox dye |
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