The Nucleotide and Deduced Amino Acid Sequences of EcoRI Fragment Containing the 5′-Terminal Region of Clostridium botulinum Type E Toxin Gene Cloned from Mashike, Iwanai and Otaru Strains

Chromosomal DNAs were extracted from toxigenic three Clostridium botulinum type E strains isolated from food-borne botulism. After digestion by EcoRI, the fragments were cloned into Escherichia coli by using bacteriophage lambda gt11 and screened with monoclonal antibody recognizing the light chain...

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Veröffentlicht in:	MICROBIOLOGY and IMMUNOLOGY 1990, Vol.34(12), pp.1041-1047
Hauptverfasser:	Fujii, Nobuhiro, Kimura, Kouichi, Murakami, Tadayuki, Indoh, Tomokazu, Yashiki, Teruo, Tsuzuki, Kayo, Yokosawa, Noriko, Oguma, Keiji
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Bacteriology Base Sequence Biological and medical sciences Cloning, Molecular Clostridium botulinum Clostridium botulinum - genetics Deoxyribonuclease EcoRI DNA, Bacterial - chemistry Escherichia coli Escherichia coli - genetics Fundamental and applied biological sciences. Psychology genes Genes, Bacterial Isopropyl Thiogalactoside Microbiology Molecular Sequence Data nucleotide sequence Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Plasmids Regulatory Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Toxoids - chemistry Toxoids - genetics Transformation, Bacterial
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