Assessment of genetic diversity among strains of Pseudomonas syringae by PCR-restriction fragment length polymorphism analysis of rRNA operons with special emphasis on P. syringae pv. tomato
Phylogenetic relationships among 77 bacterial strains belonging to Pseudomonas syringae and Pseudomonas viridiflava species were assessed by analysis of the PCR-restriction fragment length polymorphism (RFLP) patterns of three DNA fragments corresponding to rrs and rrl genes and the internal transcr...
Gespeichert in:
| Veröffentlicht in: | Applied and Environmental Microbiology 1997-02, Vol.63 (2), p.498-505 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 505 |
|---|---|
| container_issue | 2 |
| container_start_page | 498 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 63 |
| creator | Manceau, C. (Institut National de la Recherche Agronomique, Beaucouze, France.) Horvais, A |
| description | Phylogenetic relationships among 77 bacterial strains belonging to Pseudomonas syringae and Pseudomonas viridiflava species were assessed by analysis of the PCR-restriction fragment length polymorphism (RFLP) patterns of three DNA fragments corresponding to rrs and rrl genes and the internal transcribed spacer, ITS1. No difference among all strains in rrs and rrl genes was observed with 14 restriction enzymes, which confirms the close relationships existing between these two species. The nucleotidic sequence of the internal transcripted spacer (ITS1) between rrs and rrl for the P. syringae pv. syringae strain CFBP1392 was determined. Restriction maps of the PCR-amplified ITS1 region were prepared and compared for all 77 strains. Seventeen RFLP patterns, forming three main clusters, were distinguished. One contained all strains of P. syringae pv. tomato and of other pathovars which had been previously described as closely related by either pathogenicity studies or biochemical analyses. This cluster was equally far from P. viridiflava and from other P. syringae pathovars. These other pathovars of P. syringae formed a less coherent taxon |
| doi_str_mv | 10.1128/aem.63.2.498-505.1997 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_proquest_miscellaneous_15896627</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15896627</sourcerecordid><originalsourceid>FETCH-LOGICAL-c622t-84755d6027b320452fb081dcfd18f70e1324622c2c2cadce96e27ec8b19a5ab3</originalsourceid><addsrcrecordid>eNqFkt-K1DAUxoso67j6AsJCEBG8aE3SNn8uvBgGdYVBh3W9DmmadrK0SU06s_TlfDbTmWF090ZyEXLO7zsnOfmS5ArBDCHMPkjdZyTPcFZwlpawzBDn9EmyQHA-5zl5miwg5DzFuIDPkxch3EEIC0jYRXLBIc45Zovk9zIEHUKv7QhcA1pt9WgUqM1e-2DGCcje2RaE0Utjw4xsgt7VLkZlAGHyxrZSg2oCm9VN6nUEjRqNs6Dxsj2U7bRtxy0YXDf1zg9bE3ogreymYA4F_c23JXCD9i42uDcRDYNWRnZA98NWHigLNtnfbsM-A6Pr5eheJs8a2QX96rRfJrefP92urtP19y9fV8t1qgjGY8oKWpY1gZhWOYZFiZsKMlSrpkasoVCjHBcRVPOStdKcaEy1YhXispRVfpl8PJYddlWvI2DjPDoxeNNLPwknjXiYsWYrWrcXiLC8gFH__qjfPlJdL9dijkFMGM0R3aPIvjv18u7XLg5U9CYo3XXSarcLgjLKGYPkvyAqGScE0wi-eQTeuZ2PPxAEhiWPloDzFcsjpLwLwevmfE8ExWw4EQ0nSC6wiIYT0XBiNlzUXf07mrPq5LCYf3vKy6BkF11hlQlnDJekwHx-NThNyLTbe-O1kKF_0DIir49II52QrY9Vfv7gFNH40PwPrkL2wQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>205940600</pqid></control><display><type>article</type><title>Assessment of genetic diversity among strains of Pseudomonas syringae by PCR-restriction fragment length polymorphism analysis of rRNA operons with special emphasis on P. syringae pv. tomato</title><source>American Society for Microbiology</source><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Manceau, C. (Institut National de la Recherche Agronomique, Beaucouze, France.) ; Horvais, A</creator><creatorcontrib>Manceau, C. (Institut National de la Recherche Agronomique, Beaucouze, France.) ; Horvais, A</creatorcontrib><description>Phylogenetic relationships among 77 bacterial strains belonging to Pseudomonas syringae and Pseudomonas viridiflava species were assessed by analysis of the PCR-restriction fragment length polymorphism (RFLP) patterns of three DNA fragments corresponding to rrs and rrl genes and the internal transcribed spacer, ITS1. No difference among all strains in rrs and rrl genes was observed with 14 restriction enzymes, which confirms the close relationships existing between these two species. The nucleotidic sequence of the internal transcripted spacer (ITS1) between rrs and rrl for the P. syringae pv. syringae strain CFBP1392 was determined. Restriction maps of the PCR-amplified ITS1 region were prepared and compared for all 77 strains. Seventeen RFLP patterns, forming three main clusters, were distinguished. One contained all strains of P. syringae pv. tomato and of other pathovars which had been previously described as closely related by either pathogenicity studies or biochemical analyses. This cluster was equally far from P. viridiflava and from other P. syringae pathovars. These other pathovars of P. syringae formed a less coherent taxon</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.63.2.498-505.1997</identifier><identifier>PMID: 9023928</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>ADN ; Bacteria ; Bacteriology ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; CHIMIOTAXONOMIE ; Deoxyribonucleic acid ; DNA ; DNA, Ribosomal - genetics ; Ecology, environment ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; Genetics ; Life Sciences ; Lycopersicon esculentum - microbiology ; Microbiology ; Mission oriented research ; Molecular Sequence Data ; Operon - genetics ; Phylogeny ; Plant Diseases - microbiology ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Pseudomonas - classification ; Pseudomonas - genetics ; Pseudomonas - pathogenicity ; Pseudomonas syringae ; Pseudomonas viridiflava ; QUIMIOTAXONOMIA ; Restriction Mapping ; Ribonucleic acid ; RIBOSOMAS ; RIBOSOME ; RNA ; RNA, Ribosomal, 16S - genetics ; RNA, Ribosomal, 23S - genetics ; SECUENCIA NUCLEOTIDICA ; Sequence Analysis, DNA ; SEQUENCE NUCLEOTIDIQUE</subject><ispartof>Applied and Environmental Microbiology, 1997-02, Vol.63 (2), p.498-505</ispartof><rights>1997 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Feb 1997</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c622t-84755d6027b320452fb081dcfd18f70e1324622c2c2cadce96e27ec8b19a5ab3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC168340/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC168340/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2564291$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9023928$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02687317$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Manceau, C. (Institut National de la Recherche Agronomique, Beaucouze, France.)</creatorcontrib><creatorcontrib>Horvais, A</creatorcontrib><title>Assessment of genetic diversity among strains of Pseudomonas syringae by PCR-restriction fragment length polymorphism analysis of rRNA operons with special emphasis on P. syringae pv. tomato</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Phylogenetic relationships among 77 bacterial strains belonging to Pseudomonas syringae and Pseudomonas viridiflava species were assessed by analysis of the PCR-restriction fragment length polymorphism (RFLP) patterns of three DNA fragments corresponding to rrs and rrl genes and the internal transcribed spacer, ITS1. No difference among all strains in rrs and rrl genes was observed with 14 restriction enzymes, which confirms the close relationships existing between these two species. The nucleotidic sequence of the internal transcripted spacer (ITS1) between rrs and rrl for the P. syringae pv. syringae strain CFBP1392 was determined. Restriction maps of the PCR-amplified ITS1 region were prepared and compared for all 77 strains. Seventeen RFLP patterns, forming three main clusters, were distinguished. One contained all strains of P. syringae pv. tomato and of other pathovars which had been previously described as closely related by either pathogenicity studies or biochemical analyses. This cluster was equally far from P. viridiflava and from other P. syringae pathovars. These other pathovars of P. syringae formed a less coherent taxon</description><subject>ADN</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>CHIMIOTAXONOMIE</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Ribosomal - genetics</subject><subject>Ecology, environment</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>Genetics</subject><subject>Life Sciences</subject><subject>Lycopersicon esculentum - microbiology</subject><subject>Microbiology</subject><subject>Mission oriented research</subject><subject>Molecular Sequence Data</subject><subject>Operon - genetics</subject><subject>Phylogeny</subject><subject>Plant Diseases - microbiology</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Pseudomonas - classification</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas - pathogenicity</subject><subject>Pseudomonas syringae</subject><subject>Pseudomonas viridiflava</subject><subject>QUIMIOTAXONOMIA</subject><subject>Restriction Mapping</subject><subject>Ribonucleic acid</subject><subject>RIBOSOMAS</subject><subject>RIBOSOME</subject><subject>RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>RNA, Ribosomal, 23S - genetics</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>Sequence Analysis, DNA</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkt-K1DAUxoso67j6AsJCEBG8aE3SNn8uvBgGdYVBh3W9DmmadrK0SU06s_TlfDbTmWF090ZyEXLO7zsnOfmS5ArBDCHMPkjdZyTPcFZwlpawzBDn9EmyQHA-5zl5miwg5DzFuIDPkxch3EEIC0jYRXLBIc45Zovk9zIEHUKv7QhcA1pt9WgUqM1e-2DGCcje2RaE0Utjw4xsgt7VLkZlAGHyxrZSg2oCm9VN6nUEjRqNs6Dxsj2U7bRtxy0YXDf1zg9bE3ogreymYA4F_c23JXCD9i42uDcRDYNWRnZA98NWHigLNtnfbsM-A6Pr5eheJs8a2QX96rRfJrefP92urtP19y9fV8t1qgjGY8oKWpY1gZhWOYZFiZsKMlSrpkasoVCjHBcRVPOStdKcaEy1YhXispRVfpl8PJYddlWvI2DjPDoxeNNLPwknjXiYsWYrWrcXiLC8gFH__qjfPlJdL9dijkFMGM0R3aPIvjv18u7XLg5U9CYo3XXSarcLgjLKGYPkvyAqGScE0wi-eQTeuZ2PPxAEhiWPloDzFcsjpLwLwevmfE8ExWw4EQ0nSC6wiIYT0XBiNlzUXf07mrPq5LCYf3vKy6BkF11hlQlnDJekwHx-NThNyLTbe-O1kKF_0DIir49II52QrY9Vfv7gFNH40PwPrkL2wQ</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>Manceau, C. (Institut National de la Recherche Agronomique, Beaucouze, France.)</creator><creator>Horvais, A</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>1XC</scope><scope>5PM</scope></search><sort><creationdate>19970201</creationdate><title>Assessment of genetic diversity among strains of Pseudomonas syringae by PCR-restriction fragment length polymorphism analysis of rRNA operons with special emphasis on P. syringae pv. tomato</title><author>Manceau, C. (Institut National de la Recherche Agronomique, Beaucouze, France.) ; Horvais, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c622t-84755d6027b320452fb081dcfd18f70e1324622c2c2cadce96e27ec8b19a5ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>ADN</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>CHIMIOTAXONOMIE</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Ribosomal - genetics</topic><topic>Ecology, environment</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>Genetics</topic><topic>Life Sciences</topic><topic>Lycopersicon esculentum - microbiology</topic><topic>Microbiology</topic><topic>Mission oriented research</topic><topic>Molecular Sequence Data</topic><topic>Operon - genetics</topic><topic>Phylogeny</topic><topic>Plant Diseases - microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Pseudomonas - classification</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas - pathogenicity</topic><topic>Pseudomonas syringae</topic><topic>Pseudomonas viridiflava</topic><topic>QUIMIOTAXONOMIA</topic><topic>Restriction Mapping</topic><topic>Ribonucleic acid</topic><topic>RIBOSOMAS</topic><topic>RIBOSOME</topic><topic>RNA</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>RNA, Ribosomal, 23S - genetics</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>Sequence Analysis, DNA</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Manceau, C. (Institut National de la Recherche Agronomique, Beaucouze, France.)</creatorcontrib><creatorcontrib>Horvais, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Manceau, C. (Institut National de la Recherche Agronomique, Beaucouze, France.)</au><au>Horvais, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assessment of genetic diversity among strains of Pseudomonas syringae by PCR-restriction fragment length polymorphism analysis of rRNA operons with special emphasis on P. syringae pv. tomato</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>63</volume><issue>2</issue><spage>498</spage><epage>505</epage><pages>498-505</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Phylogenetic relationships among 77 bacterial strains belonging to Pseudomonas syringae and Pseudomonas viridiflava species were assessed by analysis of the PCR-restriction fragment length polymorphism (RFLP) patterns of three DNA fragments corresponding to rrs and rrl genes and the internal transcribed spacer, ITS1. No difference among all strains in rrs and rrl genes was observed with 14 restriction enzymes, which confirms the close relationships existing between these two species. The nucleotidic sequence of the internal transcripted spacer (ITS1) between rrs and rrl for the P. syringae pv. syringae strain CFBP1392 was determined. Restriction maps of the PCR-amplified ITS1 region were prepared and compared for all 77 strains. Seventeen RFLP patterns, forming three main clusters, were distinguished. One contained all strains of P. syringae pv. tomato and of other pathovars which had been previously described as closely related by either pathogenicity studies or biochemical analyses. This cluster was equally far from P. viridiflava and from other P. syringae pathovars. These other pathovars of P. syringae formed a less coherent taxon</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>9023928</pmid><doi>10.1128/aem.63.2.498-505.1997</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 1997-02, Vol.63 (2), p.498-505 |
| issn | 0099-2240 1098-5336 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15896627 |
| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | ADN Bacteria Bacteriology Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology CHIMIOTAXONOMIE Deoxyribonucleic acid DNA DNA, Ribosomal - genetics Ecology, environment Fundamental and applied biological sciences. Psychology Genetic Variation Genetics Life Sciences Lycopersicon esculentum - microbiology Microbiology Mission oriented research Molecular Sequence Data Operon - genetics Phylogeny Plant Diseases - microbiology Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Pseudomonas - classification Pseudomonas - genetics Pseudomonas - pathogenicity Pseudomonas syringae Pseudomonas viridiflava QUIMIOTAXONOMIA Restriction Mapping Ribonucleic acid RIBOSOMAS RIBOSOME RNA RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 23S - genetics SECUENCIA NUCLEOTIDICA Sequence Analysis, DNA SEQUENCE NUCLEOTIDIQUE |
| title | Assessment of genetic diversity among strains of Pseudomonas syringae by PCR-restriction fragment length polymorphism analysis of rRNA operons with special emphasis on P. syringae pv. tomato |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T20%3A19%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Assessment%20of%20genetic%20diversity%20among%20strains%20of%20Pseudomonas%20syringae%20by%20PCR-restriction%20fragment%20length%20polymorphism%20analysis%20of%20rRNA%20operons%20with%20special%20emphasis%20on%20P.%20syringae%20pv.%20tomato&rft.jtitle=Applied%20and%20Environmental%20Microbiology&rft.au=Manceau,%20C.%20(Institut%20National%20de%20la%20Recherche%20Agronomique,%20Beaucouze,%20France.)&rft.date=1997-02-01&rft.volume=63&rft.issue=2&rft.spage=498&rft.epage=505&rft.pages=498-505&rft.issn=0099-2240&rft.eissn=1098-5336&rft.coden=AEMIDF&rft_id=info:doi/10.1128/aem.63.2.498-505.1997&rft_dat=%3Cproquest_hal_p%3E15896627%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=205940600&rft_id=info:pmid/9023928&rfr_iscdi=true |