The gene promoter for a bean abscission cellulase is ethylene-induced in transgenic tomato and shows high sequence conservation with a soybean abscission cellulase
Bean leaf abscission (organ separation) correlates with the de novo accumulation of a pI9.5 cellulase and its mRNA. Overlapping genomic clones encoding the bean abscission cellulase (BAC) were isolated and partially sequenced. In addition, a genomic clone for a soybean abscission cellulase (SAC) was...
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| Veröffentlicht in: | Plant molecular biology 1996-06, Vol.31 (3), p.595-606 |
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| creator | Koehler, S.M. (Plant Molecular Biology Lab. USDA-ARS, Beltsville, MD (USA)) Matters, G.L Nath, P Kemmerer, E.C Tucker, M.L |
| description | Bean leaf abscission (organ separation) correlates with the de novo accumulation of a pI9.5 cellulase and its mRNA. Overlapping genomic clones encoding the bean abscission cellulase (BAC) were isolated and partially sequenced. In addition, a genomic clone for a soybean abscission cellulase (SAC) was identified and the sequence compared to the BAC genomic sequence. Two 5'-upstream regions are particularly well conserved in the two sequences. Of special interest here is the region between -1 and -200 in the BAC promoter which is highly conserved in the SAC gene. Particle gun bombardment with a BAC promoter construct containing 210 bp of BAC sequence 5' to the transcription start site was sufficient to drive abscission-specific and ethylene and auxin-regulated transient expression in bean. In addition to the transient expression assay, expression was examined in stably transformed tomato. A similar -210 bp BAC promoter construct supported a low level of ethylene-inducible reporter gene expression in tomato leaf abscission zones and adjacent petioles but not in ethylene-treated stem tissue or fruit. Expression from the -210 promoter in tomato abscission zones was inhibited by silver thiosulfate, an ethylene action inhibitor, and was partially inhibited by treatment with auxin. |
| doi_str_mv | 10.1007/BF00042232 |
| format | Article |
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(Plant Molecular Biology Lab. USDA-ARS, Beltsville, MD (USA)) ; Matters, G.L ; Nath, P ; Kemmerer, E.C ; Tucker, M.L</creator><creatorcontrib>Koehler, S.M. (Plant Molecular Biology Lab. USDA-ARS, Beltsville, MD (USA)) ; Matters, G.L ; Nath, P ; Kemmerer, E.C ; Tucker, M.L</creatorcontrib><description>Bean leaf abscission (organ separation) correlates with the de novo accumulation of a pI9.5 cellulase and its mRNA. Overlapping genomic clones encoding the bean abscission cellulase (BAC) were isolated and partially sequenced. In addition, a genomic clone for a soybean abscission cellulase (SAC) was identified and the sequence compared to the BAC genomic sequence. Two 5'-upstream regions are particularly well conserved in the two sequences. Of special interest here is the region between -1 and -200 in the BAC promoter which is highly conserved in the SAC gene. Particle gun bombardment with a BAC promoter construct containing 210 bp of BAC sequence 5' to the transcription start site was sufficient to drive abscission-specific and ethylene and auxin-regulated transient expression in bean. In addition to the transient expression assay, expression was examined in stably transformed tomato. A similar -210 bp BAC promoter construct supported a low level of ethylene-inducible reporter gene expression in tomato leaf abscission zones and adjacent petioles but not in ethylene-treated stem tissue or fruit. Expression from the -210 promoter in tomato abscission zones was inhibited by silver thiosulfate, an ethylene action inhibitor, and was partially inhibited by treatment with auxin.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00042232</identifier><identifier>PMID: 8790292</identifier><language>eng</language><publisher>Netherlands</publisher><subject>ABSCISION ; ABSCISSION ; Base Sequence ; CELLULASE ; Cellulase - biosynthesis ; Cellulase - chemistry ; Cellulase - genetics ; CELULASA ; Conserved Sequence ; ETHYLENE ; Ethylenes - pharmacology ; ETILENO ; Fabaceae - enzymology ; Fabaceae - genetics ; FEUILLE ; GENE ; GENES ; Genes, Reporter - drug effects ; Glycine max ; Glycine max - enzymology ; Glycine max - genetics ; HOJAS ; Indoleacetic Acids - pharmacology ; LEAVES ; Luciferases - biosynthesis ; Molecular Sequence Data ; NUCLEOTIDE SEQUENCE ; PHASEOLUS VULGARIS ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants, Genetically Modified ; Plants, Medicinal ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; Recombinant Fusion Proteins - biosynthesis ; RNA, Messenger - biosynthesis ; SECUENCIA NUCLEOTIDICA ; Sequence Homology, Nucleic Acid ; SEQUENCE NUCLEOTIDIQUE ; Solanum lycopersicum ; Soybean Proteins ; Thiosulfates - pharmacology ; Transcription, Genetic - drug effects ; TRANSGENIC PLANTS</subject><ispartof>Plant molecular biology, 1996-06, Vol.31 (3), p.595-606</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-5c1444fd30f688714dcfb08572b2e732c8237373d05e8958b0b1fac09d6371803</citedby><cites>FETCH-LOGICAL-c332t-5c1444fd30f688714dcfb08572b2e732c8237373d05e8958b0b1fac09d6371803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8790292$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koehler, S.M. (Plant Molecular Biology Lab. USDA-ARS, Beltsville, MD (USA))</creatorcontrib><creatorcontrib>Matters, G.L</creatorcontrib><creatorcontrib>Nath, P</creatorcontrib><creatorcontrib>Kemmerer, E.C</creatorcontrib><creatorcontrib>Tucker, M.L</creatorcontrib><title>The gene promoter for a bean abscission cellulase is ethylene-induced in transgenic tomato and shows high sequence conservation with a soybean abscission cellulase</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Bean leaf abscission (organ separation) correlates with the de novo accumulation of a pI9.5 cellulase and its mRNA. Overlapping genomic clones encoding the bean abscission cellulase (BAC) were isolated and partially sequenced. In addition, a genomic clone for a soybean abscission cellulase (SAC) was identified and the sequence compared to the BAC genomic sequence. Two 5'-upstream regions are particularly well conserved in the two sequences. Of special interest here is the region between -1 and -200 in the BAC promoter which is highly conserved in the SAC gene. Particle gun bombardment with a BAC promoter construct containing 210 bp of BAC sequence 5' to the transcription start site was sufficient to drive abscission-specific and ethylene and auxin-regulated transient expression in bean. In addition to the transient expression assay, expression was examined in stably transformed tomato. A similar -210 bp BAC promoter construct supported a low level of ethylene-inducible reporter gene expression in tomato leaf abscission zones and adjacent petioles but not in ethylene-treated stem tissue or fruit. Expression from the -210 promoter in tomato abscission zones was inhibited by silver thiosulfate, an ethylene action inhibitor, and was partially inhibited by treatment with auxin.</description><subject>ABSCISION</subject><subject>ABSCISSION</subject><subject>Base Sequence</subject><subject>CELLULASE</subject><subject>Cellulase - biosynthesis</subject><subject>Cellulase - chemistry</subject><subject>Cellulase - genetics</subject><subject>CELULASA</subject><subject>Conserved Sequence</subject><subject>ETHYLENE</subject><subject>Ethylenes - pharmacology</subject><subject>ETILENO</subject><subject>Fabaceae - enzymology</subject><subject>Fabaceae - genetics</subject><subject>FEUILLE</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Reporter - drug effects</subject><subject>Glycine max</subject><subject>Glycine max - enzymology</subject><subject>Glycine max - genetics</subject><subject>HOJAS</subject><subject>Indoleacetic Acids - pharmacology</subject><subject>LEAVES</subject><subject>Luciferases - biosynthesis</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>PHASEOLUS VULGARIS</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants, Genetically Modified</subject><subject>Plants, Medicinal</subject><subject>Polymerase Chain Reaction</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>Solanum lycopersicum</subject><subject>Soybean Proteins</subject><subject>Thiosulfates - pharmacology</subject><subject>Transcription, Genetic - drug effects</subject><subject>TRANSGENIC PLANTS</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUFv1DAQhS1EVZbChSMSkk8ckFLGdhI7R1rRUmkFl3KOHGeyMUrs4kmo9vfwR_FqV_RUzWEO882bp3mMvRNwKQD056sbACilVPIF24hKq6ICaV6yDYhaF2Up5Cv2mugXQMZVfc7OjW5ANnLD_t6PyHcYkD-kOMcFEx9i4pZ3aAO3HTlP5GPgDqdpnSwh98RxGfdTXip86FeHPfeBL8kGykre8SXOdonchp7TGB-Jj343csLfKwaH3MVAmP7Y5aD76Jcxn6O4f_biG3Y22Inw7alfsJ83X--vvxXbH7d311-2hVNKLkXlRFmWQ69gqI3Rouzd0IGptOwkaiWdkUrn6qFC01Smg04M1kHT10oLA-qCfTzq5ldkq7S0s6eDCxswrtSKyjRlUzUZ_HQEXYpECYf2IfnZpn0roD0k0j4lkuEPJ9W1m7H_j54iyPP3x_lgY2t3yVP7fdtoEGWt1D8lWpCb</recordid><startdate>19960601</startdate><enddate>19960601</enddate><creator>Koehler, S.M. 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USDA-ARS, Beltsville, MD (USA)) ; Matters, G.L ; Nath, P ; Kemmerer, E.C ; Tucker, M.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-5c1444fd30f688714dcfb08572b2e732c8237373d05e8958b0b1fac09d6371803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ABSCISION</topic><topic>ABSCISSION</topic><topic>Base Sequence</topic><topic>CELLULASE</topic><topic>Cellulase - biosynthesis</topic><topic>Cellulase - chemistry</topic><topic>Cellulase - genetics</topic><topic>CELULASA</topic><topic>Conserved Sequence</topic><topic>ETHYLENE</topic><topic>Ethylenes - pharmacology</topic><topic>ETILENO</topic><topic>Fabaceae - enzymology</topic><topic>Fabaceae - genetics</topic><topic>FEUILLE</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Reporter - drug effects</topic><topic>Glycine max</topic><topic>Glycine max - enzymology</topic><topic>Glycine max - genetics</topic><topic>HOJAS</topic><topic>Indoleacetic Acids - pharmacology</topic><topic>LEAVES</topic><topic>Luciferases - biosynthesis</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>PHASEOLUS VULGARIS</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants, Genetically Modified</topic><topic>Plants, Medicinal</topic><topic>Polymerase Chain Reaction</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Fusion Proteins - biosynthesis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>Solanum lycopersicum</topic><topic>Soybean Proteins</topic><topic>Thiosulfates - pharmacology</topic><topic>Transcription, Genetic - drug effects</topic><topic>TRANSGENIC PLANTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koehler, S.M. (Plant Molecular Biology Lab. USDA-ARS, Beltsville, MD (USA))</creatorcontrib><creatorcontrib>Matters, G.L</creatorcontrib><creatorcontrib>Nath, P</creatorcontrib><creatorcontrib>Kemmerer, E.C</creatorcontrib><creatorcontrib>Tucker, M.L</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koehler, S.M. (Plant Molecular Biology Lab. USDA-ARS, Beltsville, MD (USA))</au><au>Matters, G.L</au><au>Nath, P</au><au>Kemmerer, E.C</au><au>Tucker, M.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The gene promoter for a bean abscission cellulase is ethylene-induced in transgenic tomato and shows high sequence conservation with a soybean abscission cellulase</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1996-06-01</date><risdate>1996</risdate><volume>31</volume><issue>3</issue><spage>595</spage><epage>606</epage><pages>595-606</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Bean leaf abscission (organ separation) correlates with the de novo accumulation of a pI9.5 cellulase and its mRNA. Overlapping genomic clones encoding the bean abscission cellulase (BAC) were isolated and partially sequenced. In addition, a genomic clone for a soybean abscission cellulase (SAC) was identified and the sequence compared to the BAC genomic sequence. Two 5'-upstream regions are particularly well conserved in the two sequences. Of special interest here is the region between -1 and -200 in the BAC promoter which is highly conserved in the SAC gene. Particle gun bombardment with a BAC promoter construct containing 210 bp of BAC sequence 5' to the transcription start site was sufficient to drive abscission-specific and ethylene and auxin-regulated transient expression in bean. In addition to the transient expression assay, expression was examined in stably transformed tomato. A similar -210 bp BAC promoter construct supported a low level of ethylene-inducible reporter gene expression in tomato leaf abscission zones and adjacent petioles but not in ethylene-treated stem tissue or fruit. Expression from the -210 promoter in tomato abscission zones was inhibited by silver thiosulfate, an ethylene action inhibitor, and was partially inhibited by treatment with auxin.</abstract><cop>Netherlands</cop><pmid>8790292</pmid><doi>10.1007/BF00042232</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1996-06, Vol.31 (3), p.595-606 |
| issn | 0167-4412 1573-5028 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15894959 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | ABSCISION ABSCISSION Base Sequence CELLULASE Cellulase - biosynthesis Cellulase - chemistry Cellulase - genetics CELULASA Conserved Sequence ETHYLENE Ethylenes - pharmacology ETILENO Fabaceae - enzymology Fabaceae - genetics FEUILLE GENE GENES Genes, Reporter - drug effects Glycine max Glycine max - enzymology Glycine max - genetics HOJAS Indoleacetic Acids - pharmacology LEAVES Luciferases - biosynthesis Molecular Sequence Data NUCLEOTIDE SEQUENCE PHASEOLUS VULGARIS PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants, Genetically Modified Plants, Medicinal Polymerase Chain Reaction Promoter Regions, Genetic Recombinant Fusion Proteins - biosynthesis RNA, Messenger - biosynthesis SECUENCIA NUCLEOTIDICA Sequence Homology, Nucleic Acid SEQUENCE NUCLEOTIDIQUE Solanum lycopersicum Soybean Proteins Thiosulfates - pharmacology Transcription, Genetic - drug effects TRANSGENIC PLANTS |
| title | The gene promoter for a bean abscission cellulase is ethylene-induced in transgenic tomato and shows high sequence conservation with a soybean abscission cellulase |
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