Detection of virus-associated dsRNA from leafroll infected grapevines

A simple procedure is described for reproducible detection of double stranded (ds) RNAs in leafroll infected grapevines. The procedure involves the extraction of tissues by a medium which preferentially yields dsRNA. The RNA is purified by CF11 cellulose chromatography and gel electrophoresis. The d...

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Veröffentlicht in:	Journal of virological methods 1991, Vol.31 (2), p.325-334
Hauptverfasser:	Rezaian, M.A., Krake, L.R., Cunying, Q., Hazzalin, C.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Chromatography diagnostic techniques double-stranded RNA dsRNA Electrophoresis Fundamental and applied biological sciences. Psychology Grapevine grapevine leaf roll virus isolation Leafroll Microbiology Plant Diseases Plant Viruses - genetics Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Reproducibility of Results RNA RNA, Double-Stranded - isolation & purification Virology Vitis vinifera
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