Quantitation of chloramphenicol acetyl transferase in transgenic tobacco plants by ELISA and correlation with gene copy number

A monoclonal antibody to chloramphenicol acetyl transferase (CAT) was used in an indirect competitive enzyme immunoassay (ELISA) for the quantitation of CAT in leaf extracts of eighteen transgenic tobacco plants containing the CAT gene fused to the cauliflower mosaic virus 35S promoter. The ELISA co...

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Veröffentlicht in:	Plant molecular biology 1990-04, Vol.14 (4), p.575-583
Hauptverfasser:	Gendloff, E.H. (Agrigenetics Advanced Science Co., Madison, WI (USA)), Bowen, B, Buchholz, W.G
Format:	Artikel
Sprache:	eng
Schlagworte:	ANTIBIOTICOS ANTIBIOTICS ANTIBIOTIQUE Biological and medical sciences Chloramphenicol O-Acetyltransferase - analysis Chloramphenicol O-Acetyltransferase - genetics Cloning, Molecular Diverse techniques ELISA Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology GENE Gene Amplification Gene Expression GENES Genetic Engineering Molecular and cellular biology Nicotiana - enzymology Nicotiana - genetics NICOTIANA TABACUM Plants - enzymology Plants - genetics Plants, Toxic TEST ELISA TRANSFERASAS TRANSFERASE TRANSFERASES
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