Origins of myo-inositol tetrakisphosphates in agonist-stimulated rat pancreatoma cells. Stimulation by bombesin of myo-inositol 1,3,4,5,6-pentakisphosphate breakdown to myo-inositol 3,4,5,6-tetrakisphosphate
In the rat pancreatoma cell line, AR4-2J, three inositol tetrakisphosphate isomers were identified, (1,3,4,6), (1,3,4,5), (3,4,5,6), which were increased during activation of phospholipase C by bombesin. Two other isomers were identified, (1,4,5,6) and a fifth isomer which was either (1,2,3,4) or (1...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 1990-07, Vol.265 (19), p.11167-11176 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 11176 |
|---|---|
| container_issue | 19 |
| container_start_page | 11167 |
| container_title | The Journal of biological chemistry |
| container_volume | 265 |
| creator | F S Menniti K G Oliver K Nogimori J F Obie S B Shears J W Putney, Jr |
| description | In the rat pancreatoma cell line, AR4-2J, three inositol tetrakisphosphate isomers were identified, (1,3,4,6), (1,3,4,5),
(3,4,5,6), which were increased during activation of phospholipase C by bombesin. Two other isomers were identified, (1,4,5,6)
and a fifth isomer which was either (1,2,3,4) or (1,2,3,6), which have not previously been detected in any cell type. To study
the metabolic interrelationships between these compounds and inositol 1,3,4,5,6-pentakisphosphate in the intact cell, their
turnover was assessed under different protocols of [3H]myo-inositol labeling; the inositol phosphates were labeled to near
steady state or under conditions where either rapidly or slowly turning over inositol polyphosphates were preferentially labeled.
The relative specific radioactivities of inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, inositol 1,3,4-trisphosphate,
and inositol 1,3,4,6-tetrakisphosphate were very similar in bombesin-stimulated cells, consistent with the pathway for the
conversion of inositol 1,4,5-trisphosphate to the other three inositol polyphosphates. Compared with these inositol phosphates,
the turnover of inositol 1,3,4,5,6-pentakisphosphate was slow. An accumulation of radioactivity into inositol 1,3,4,5,6-pentakisphosphate
was observed only under labeling conditions where its relative specific radioactivity was substantially below that of inositol
1,3,4,6-tetrakisphosphate. This indicated that the precursor for de novo synthesis of inositol 1,3,4,5,6-pentakisphosphate
was inositol 1,3,4,6-tetrakisphosphate. Bombesin stimulated the net breakdown of inositol 1,3,4,5,6-pentakisphosphate and
increased the level of inositol 3,4,5,6-tetrakisphosphate; the relative specific radioactivities of these two compounds were
similar under all conditions. These data led to the novel proposal that inositol 3,4,5,6-tetrakisphosphate is the product
of inositol 1,3,4,5,6-pentakisphosphate breakdown. This reaction was apparently stimulated by a regulated change in the enzyme(s)
which interconvert inositol 1,3,4,5,6-pentakisphosphate and inositol 3,4,5,6-tetrakisphosphate. |
| doi_str_mv | 10.1016/S0021-9258(19)38572-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_15888209</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15888209</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3262-1ca61183570dcabaa9d49ae2908671142c7543e0d0b91f653f33e9a5bb839b443</originalsourceid><addsrcrecordid>eNplkd1qHCEYhqW0pNu0lxDwoJQWdlI_HR09LCFpC4EcJIWeic44uzYzOlWXsFeZW8psdhtII4jg9_4oD0InQE6BgPh6TQiFSlEuP4P6wiRvaEVfoQUQySrG4fdrtHiSvEXvcv5D5lUrOEJHlHFZc7FA91fJr3zIOPZ43MbKh5h9iQMuriRz6_O0jvM2xWXsAzarGHwuVS5-3AzzbYeTKXgyoU3OlDga3LphyKf4-qDwMWC7xTaO1uU54f8eWLJlveRLUU0ulGeN2M6Zt128C7jE565_nhevfI_e9GbI7sPhPEa_Ls5vzn5Ul1fff559u6xaRgWtoDUCQDLekK411hjV1co4qogUDUBN24bXzJGOWAW94KxnzCnDrZVM2bpmx-jTPndK8e_G5aJHn3dfN8HFTdbApZSUqFnI98I2xZyT6_WU_GjSVgPRO5D6EaTeUdKg9CNITWffyaFgY0fXPbkO5Ob5x_187VfrO5-ctj62azdqKvguCABEwx4AXsypQg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15888209</pqid></control><display><type>article</type><title>Origins of myo-inositol tetrakisphosphates in agonist-stimulated rat pancreatoma cells. Stimulation by bombesin of myo-inositol 1,3,4,5,6-pentakisphosphate breakdown to myo-inositol 3,4,5,6-tetrakisphosphate</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>F S Menniti ; K G Oliver ; K Nogimori ; J F Obie ; S B Shears ; J W Putney, Jr</creator><creatorcontrib>F S Menniti ; K G Oliver ; K Nogimori ; J F Obie ; S B Shears ; J W Putney, Jr</creatorcontrib><description>In the rat pancreatoma cell line, AR4-2J, three inositol tetrakisphosphate isomers were identified, (1,3,4,6), (1,3,4,5),
(3,4,5,6), which were increased during activation of phospholipase C by bombesin. Two other isomers were identified, (1,4,5,6)
and a fifth isomer which was either (1,2,3,4) or (1,2,3,6), which have not previously been detected in any cell type. To study
the metabolic interrelationships between these compounds and inositol 1,3,4,5,6-pentakisphosphate in the intact cell, their
turnover was assessed under different protocols of [3H]myo-inositol labeling; the inositol phosphates were labeled to near
steady state or under conditions where either rapidly or slowly turning over inositol polyphosphates were preferentially labeled.
The relative specific radioactivities of inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, inositol 1,3,4-trisphosphate,
and inositol 1,3,4,6-tetrakisphosphate were very similar in bombesin-stimulated cells, consistent with the pathway for the
conversion of inositol 1,4,5-trisphosphate to the other three inositol polyphosphates. Compared with these inositol phosphates,
the turnover of inositol 1,3,4,5,6-pentakisphosphate was slow. An accumulation of radioactivity into inositol 1,3,4,5,6-pentakisphosphate
was observed only under labeling conditions where its relative specific radioactivity was substantially below that of inositol
1,3,4,6-tetrakisphosphate. This indicated that the precursor for de novo synthesis of inositol 1,3,4,5,6-pentakisphosphate
was inositol 1,3,4,6-tetrakisphosphate. Bombesin stimulated the net breakdown of inositol 1,3,4,5,6-pentakisphosphate and
increased the level of inositol 3,4,5,6-tetrakisphosphate; the relative specific radioactivities of these two compounds were
similar under all conditions. These data led to the novel proposal that inositol 3,4,5,6-tetrakisphosphate is the product
of inositol 1,3,4,5,6-pentakisphosphate breakdown. This reaction was apparently stimulated by a regulated change in the enzyme(s)
which interconvert inositol 1,3,4,5,6-pentakisphosphate and inositol 3,4,5,6-tetrakisphosphate.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/S0021-9258(19)38572-2</identifier><identifier>PMID: 2358456</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Animals ; bombesin ; Bombesin - pharmacology ; Chromatography, High Pressure Liquid ; Inositol - metabolism ; Inositol 1,4,5-Trisphosphate - metabolism ; inositol 3,4,5,6-tetrakisphosphate ; Inositol Phosphates - metabolism ; Kinetics ; myo-inositol 1,3,4,5,6-pentakisphosphate ; Pancreatic Neoplasms - metabolism ; Rats ; Tumor Cells, Cultured</subject><ispartof>The Journal of biological chemistry, 1990-07, Vol.265 (19), p.11167-11176</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3262-1ca61183570dcabaa9d49ae2908671142c7543e0d0b91f653f33e9a5bb839b443</citedby><cites>FETCH-LOGICAL-c3262-1ca61183570dcabaa9d49ae2908671142c7543e0d0b91f653f33e9a5bb839b443</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2358456$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>F S Menniti</creatorcontrib><creatorcontrib>K G Oliver</creatorcontrib><creatorcontrib>K Nogimori</creatorcontrib><creatorcontrib>J F Obie</creatorcontrib><creatorcontrib>S B Shears</creatorcontrib><creatorcontrib>J W Putney, Jr</creatorcontrib><title>Origins of myo-inositol tetrakisphosphates in agonist-stimulated rat pancreatoma cells. Stimulation by bombesin of myo-inositol 1,3,4,5,6-pentakisphosphate breakdown to myo-inositol 3,4,5,6-tetrakisphosphate</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>In the rat pancreatoma cell line, AR4-2J, three inositol tetrakisphosphate isomers were identified, (1,3,4,6), (1,3,4,5),
(3,4,5,6), which were increased during activation of phospholipase C by bombesin. Two other isomers were identified, (1,4,5,6)
and a fifth isomer which was either (1,2,3,4) or (1,2,3,6), which have not previously been detected in any cell type. To study
the metabolic interrelationships between these compounds and inositol 1,3,4,5,6-pentakisphosphate in the intact cell, their
turnover was assessed under different protocols of [3H]myo-inositol labeling; the inositol phosphates were labeled to near
steady state or under conditions where either rapidly or slowly turning over inositol polyphosphates were preferentially labeled.
The relative specific radioactivities of inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, inositol 1,3,4-trisphosphate,
and inositol 1,3,4,6-tetrakisphosphate were very similar in bombesin-stimulated cells, consistent with the pathway for the
conversion of inositol 1,4,5-trisphosphate to the other three inositol polyphosphates. Compared with these inositol phosphates,
the turnover of inositol 1,3,4,5,6-pentakisphosphate was slow. An accumulation of radioactivity into inositol 1,3,4,5,6-pentakisphosphate
was observed only under labeling conditions where its relative specific radioactivity was substantially below that of inositol
1,3,4,6-tetrakisphosphate. This indicated that the precursor for de novo synthesis of inositol 1,3,4,5,6-pentakisphosphate
was inositol 1,3,4,6-tetrakisphosphate. Bombesin stimulated the net breakdown of inositol 1,3,4,5,6-pentakisphosphate and
increased the level of inositol 3,4,5,6-tetrakisphosphate; the relative specific radioactivities of these two compounds were
similar under all conditions. These data led to the novel proposal that inositol 3,4,5,6-tetrakisphosphate is the product
of inositol 1,3,4,5,6-pentakisphosphate breakdown. This reaction was apparently stimulated by a regulated change in the enzyme(s)
which interconvert inositol 1,3,4,5,6-pentakisphosphate and inositol 3,4,5,6-tetrakisphosphate.</description><subject>Animals</subject><subject>bombesin</subject><subject>Bombesin - pharmacology</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Inositol - metabolism</subject><subject>Inositol 1,4,5-Trisphosphate - metabolism</subject><subject>inositol 3,4,5,6-tetrakisphosphate</subject><subject>Inositol Phosphates - metabolism</subject><subject>Kinetics</subject><subject>myo-inositol 1,3,4,5,6-pentakisphosphate</subject><subject>Pancreatic Neoplasms - metabolism</subject><subject>Rats</subject><subject>Tumor Cells, Cultured</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkd1qHCEYhqW0pNu0lxDwoJQWdlI_HR09LCFpC4EcJIWeic44uzYzOlWXsFeZW8psdhtII4jg9_4oD0InQE6BgPh6TQiFSlEuP4P6wiRvaEVfoQUQySrG4fdrtHiSvEXvcv5D5lUrOEJHlHFZc7FA91fJr3zIOPZ43MbKh5h9iQMuriRz6_O0jvM2xWXsAzarGHwuVS5-3AzzbYeTKXgyoU3OlDga3LphyKf4-qDwMWC7xTaO1uU54f8eWLJlveRLUU0ulGeN2M6Zt128C7jE565_nhevfI_e9GbI7sPhPEa_Ls5vzn5Ul1fff559u6xaRgWtoDUCQDLekK411hjV1co4qogUDUBN24bXzJGOWAW94KxnzCnDrZVM2bpmx-jTPndK8e_G5aJHn3dfN8HFTdbApZSUqFnI98I2xZyT6_WU_GjSVgPRO5D6EaTeUdKg9CNITWffyaFgY0fXPbkO5Ob5x_187VfrO5-ctj62azdqKvguCABEwx4AXsypQg</recordid><startdate>19900705</startdate><enddate>19900705</enddate><creator>F S Menniti</creator><creator>K G Oliver</creator><creator>K Nogimori</creator><creator>J F Obie</creator><creator>S B Shears</creator><creator>J W Putney, Jr</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope></search><sort><creationdate>19900705</creationdate><title>Origins of myo-inositol tetrakisphosphates in agonist-stimulated rat pancreatoma cells. Stimulation by bombesin of myo-inositol 1,3,4,5,6-pentakisphosphate breakdown to myo-inositol 3,4,5,6-tetrakisphosphate</title><author>F S Menniti ; K G Oliver ; K Nogimori ; J F Obie ; S B Shears ; J W Putney, Jr</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3262-1ca61183570dcabaa9d49ae2908671142c7543e0d0b91f653f33e9a5bb839b443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>bombesin</topic><topic>Bombesin - pharmacology</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Inositol - metabolism</topic><topic>Inositol 1,4,5-Trisphosphate - metabolism</topic><topic>inositol 3,4,5,6-tetrakisphosphate</topic><topic>Inositol Phosphates - metabolism</topic><topic>Kinetics</topic><topic>myo-inositol 1,3,4,5,6-pentakisphosphate</topic><topic>Pancreatic Neoplasms - metabolism</topic><topic>Rats</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>F S Menniti</creatorcontrib><creatorcontrib>K G Oliver</creatorcontrib><creatorcontrib>K Nogimori</creatorcontrib><creatorcontrib>J F Obie</creatorcontrib><creatorcontrib>S B Shears</creatorcontrib><creatorcontrib>J W Putney, Jr</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>F S Menniti</au><au>K G Oliver</au><au>K Nogimori</au><au>J F Obie</au><au>S B Shears</au><au>J W Putney, Jr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Origins of myo-inositol tetrakisphosphates in agonist-stimulated rat pancreatoma cells. Stimulation by bombesin of myo-inositol 1,3,4,5,6-pentakisphosphate breakdown to myo-inositol 3,4,5,6-tetrakisphosphate</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1990-07-05</date><risdate>1990</risdate><volume>265</volume><issue>19</issue><spage>11167</spage><epage>11176</epage><pages>11167-11176</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>In the rat pancreatoma cell line, AR4-2J, three inositol tetrakisphosphate isomers were identified, (1,3,4,6), (1,3,4,5),
(3,4,5,6), which were increased during activation of phospholipase C by bombesin. Two other isomers were identified, (1,4,5,6)
and a fifth isomer which was either (1,2,3,4) or (1,2,3,6), which have not previously been detected in any cell type. To study
the metabolic interrelationships between these compounds and inositol 1,3,4,5,6-pentakisphosphate in the intact cell, their
turnover was assessed under different protocols of [3H]myo-inositol labeling; the inositol phosphates were labeled to near
steady state or under conditions where either rapidly or slowly turning over inositol polyphosphates were preferentially labeled.
The relative specific radioactivities of inositol 1,4,5-trisphosphate, inositol 1,3,4,5-tetrakisphosphate, inositol 1,3,4-trisphosphate,
and inositol 1,3,4,6-tetrakisphosphate were very similar in bombesin-stimulated cells, consistent with the pathway for the
conversion of inositol 1,4,5-trisphosphate to the other three inositol polyphosphates. Compared with these inositol phosphates,
the turnover of inositol 1,3,4,5,6-pentakisphosphate was slow. An accumulation of radioactivity into inositol 1,3,4,5,6-pentakisphosphate
was observed only under labeling conditions where its relative specific radioactivity was substantially below that of inositol
1,3,4,6-tetrakisphosphate. This indicated that the precursor for de novo synthesis of inositol 1,3,4,5,6-pentakisphosphate
was inositol 1,3,4,6-tetrakisphosphate. Bombesin stimulated the net breakdown of inositol 1,3,4,5,6-pentakisphosphate and
increased the level of inositol 3,4,5,6-tetrakisphosphate; the relative specific radioactivities of these two compounds were
similar under all conditions. These data led to the novel proposal that inositol 3,4,5,6-tetrakisphosphate is the product
of inositol 1,3,4,5,6-pentakisphosphate breakdown. This reaction was apparently stimulated by a regulated change in the enzyme(s)
which interconvert inositol 1,3,4,5,6-pentakisphosphate and inositol 3,4,5,6-tetrakisphosphate.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>2358456</pmid><doi>10.1016/S0021-9258(19)38572-2</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1990-07, Vol.265 (19), p.11167-11176 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15888209 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals bombesin Bombesin - pharmacology Chromatography, High Pressure Liquid Inositol - metabolism Inositol 1,4,5-Trisphosphate - metabolism inositol 3,4,5,6-tetrakisphosphate Inositol Phosphates - metabolism Kinetics myo-inositol 1,3,4,5,6-pentakisphosphate Pancreatic Neoplasms - metabolism Rats Tumor Cells, Cultured |
| title | Origins of myo-inositol tetrakisphosphates in agonist-stimulated rat pancreatoma cells. Stimulation by bombesin of myo-inositol 1,3,4,5,6-pentakisphosphate breakdown to myo-inositol 3,4,5,6-tetrakisphosphate |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T03%3A34%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Origins%20of%20myo-inositol%20tetrakisphosphates%20in%20agonist-stimulated%20rat%20pancreatoma%20cells.%20Stimulation%20by%20bombesin%20of%20myo-inositol%201,3,4,5,6-pentakisphosphate%20breakdown%20to%20myo-inositol%203,4,5,6-tetrakisphosphate&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=F%20S%20Menniti&rft.date=1990-07-05&rft.volume=265&rft.issue=19&rft.spage=11167&rft.epage=11176&rft.pages=11167-11176&rft.issn=0021-9258&rft.eissn=1083-351X&rft_id=info:doi/10.1016/S0021-9258(19)38572-2&rft_dat=%3Cproquest_cross%3E15888209%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15888209&rft_id=info:pmid/2358456&rfr_iscdi=true |