Rapid identification of Rhizobium species based on cellular fatty acid analysis
As understanding of the evolutionary relationships between strains and species of root nodule bacteria increases the need for a rapid identification method that correlates well with phylogenetic relationships is clear. We have examined 123 strains of Rhizobium: R. fredii (19), R. galegae (20), R. le...
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| Veröffentlicht in: | Plant and soil 1994-04, Vol.161 (1), p.31-41 |
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| creator | Jarvis, B.D.W. (Massey Univ., Palmerston North (New Zealand). Dept. of Microbiology and Genetics) Tighe, S.W |
| description | As understanding of the evolutionary relationships between strains and species of root nodule bacteria increases the need for a rapid identification method that correlates well with phylogenetic relationships is clear. We have examined 123 strains of Rhizobium: R. fredii (19), R. galegae (20), R. leguminosarum (22), R. loti (17), R. meliloti (21), and R. tropici (18) and six unknowns. All strains were grown on modified tryptone yeast-extract (TY) agar, as log phase cultures, scraped from the agar, lysed, and the released fatty acids derivatized to their corresponding methyl esters. The methyl esters were analysed by gas-chromatography using the MIDI/Hewlett-Packard Microbial Identification System. All species studied contained 16:0, 17:0, 18:0 and 19cyclow9C fatty acids but only R loti and R tropici produced 12:0 3 OH, 13:0 iso 3 OH,18:1w9C and 15:0 iso 3 OH,17:0 iso 3 OH and 20:2w6,9C fatty acids respectively. Principal component analysis was used to show that strains could be divided into clusters corresponding to the six species. Fatty acid profiles for each species were developed and these correctly identified at least 95% of the strains belonging to each species. A dendrogram is presented showing the relationships between Rhizobium species based on fatty acid composition. The data base was used to identify unknown soil isolates as strains of Rhizobium lacking a symbiotic plasmid and a bacterium capable of expressing a symbiotic plasmid from R. leguminosarum as Sphingobacterium spiritovorum. |
| doi_str_mv | 10.1007/BF02183083 |
| format | Article |
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(Massey Univ., Palmerston North (New Zealand). Dept. of Microbiology and Genetics) ; Tighe, S.W</creator><creatorcontrib>Jarvis, B.D.W. (Massey Univ., Palmerston North (New Zealand). Dept. of Microbiology and Genetics) ; Tighe, S.W</creatorcontrib><description>As understanding of the evolutionary relationships between strains and species of root nodule bacteria increases the need for a rapid identification method that correlates well with phylogenetic relationships is clear. We have examined 123 strains of Rhizobium: R. fredii (19), R. galegae (20), R. leguminosarum (22), R. loti (17), R. meliloti (21), and R. tropici (18) and six unknowns. All strains were grown on modified tryptone yeast-extract (TY) agar, as log phase cultures, scraped from the agar, lysed, and the released fatty acids derivatized to their corresponding methyl esters. The methyl esters were analysed by gas-chromatography using the MIDI/Hewlett-Packard Microbial Identification System. All species studied contained 16:0, 17:0, 18:0 and 19cyclow9C fatty acids but only R loti and R tropici produced 12:0 3 OH, 13:0 iso 3 OH,18:1w9C and 15:0 iso 3 OH,17:0 iso 3 OH and 20:2w6,9C fatty acids respectively. Principal component analysis was used to show that strains could be divided into clusters corresponding to the six species. Fatty acid profiles for each species were developed and these correctly identified at least 95% of the strains belonging to each species. A dendrogram is presented showing the relationships between Rhizobium species based on fatty acid composition. The data base was used to identify unknown soil isolates as strains of Rhizobium lacking a symbiotic plasmid and a bacterium capable of expressing a symbiotic plasmid from R. leguminosarum as Sphingobacterium spiritovorum.</description><identifier>ISSN: 0032-079X</identifier><identifier>EISSN: 1573-5036</identifier><identifier>DOI: 10.1007/BF02183083</identifier><identifier>CODEN: PLSOA2</identifier><language>eng</language><publisher>Dordrecht: Kluwer Academic Publishers</publisher><subject>ACIDE GRAS ; ACIDOS GRASOS ; Agronomy. Soil science and plant productions ; Bacteria ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biochemistry and biology ; Biological and medical sciences ; Biological taxonomies ; Chemical, physicochemical, biochemical and biological properties ; Esters ; FATTY ACIDS ; Fundamental and applied biological sciences. Psychology ; IDENTIFICACION ; IDENTIFICATION ; Microbiology ; New species ; Phylogenetics ; Physics, chemistry, biochemistry and biology of agricultural and forest soils ; RHIZOBIUM ; Root nodules ; SIMBIOSIS ; Soil science ; Sphingobacteria ; SYMBIOSE ; SYMBIOSIS</subject><ispartof>Plant and soil, 1994-04, Vol.161 (1), p.31-41</ispartof><rights>1994 Kluwer Academic Publishers</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/42939484$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/42939484$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>309,310,314,776,780,785,786,799,23909,23910,25118,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4111905$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Jarvis, B.D.W. (Massey Univ., Palmerston North (New Zealand). Dept. of Microbiology and Genetics)</creatorcontrib><creatorcontrib>Tighe, S.W</creatorcontrib><title>Rapid identification of Rhizobium species based on cellular fatty acid analysis</title><title>Plant and soil</title><description>As understanding of the evolutionary relationships between strains and species of root nodule bacteria increases the need for a rapid identification method that correlates well with phylogenetic relationships is clear. We have examined 123 strains of Rhizobium: R. fredii (19), R. galegae (20), R. leguminosarum (22), R. loti (17), R. meliloti (21), and R. tropici (18) and six unknowns. All strains were grown on modified tryptone yeast-extract (TY) agar, as log phase cultures, scraped from the agar, lysed, and the released fatty acids derivatized to their corresponding methyl esters. The methyl esters were analysed by gas-chromatography using the MIDI/Hewlett-Packard Microbial Identification System. All species studied contained 16:0, 17:0, 18:0 and 19cyclow9C fatty acids but only R loti and R tropici produced 12:0 3 OH, 13:0 iso 3 OH,18:1w9C and 15:0 iso 3 OH,17:0 iso 3 OH and 20:2w6,9C fatty acids respectively. Principal component analysis was used to show that strains could be divided into clusters corresponding to the six species. Fatty acid profiles for each species were developed and these correctly identified at least 95% of the strains belonging to each species. A dendrogram is presented showing the relationships between Rhizobium species based on fatty acid composition. The data base was used to identify unknown soil isolates as strains of Rhizobium lacking a symbiotic plasmid and a bacterium capable of expressing a symbiotic plasmid from R. leguminosarum as Sphingobacterium spiritovorum.</description><subject>ACIDE GRAS</subject><subject>ACIDOS GRASOS</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Bacteria</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biochemistry and biology</subject><subject>Biological and medical sciences</subject><subject>Biological taxonomies</subject><subject>Chemical, physicochemical, biochemical and biological properties</subject><subject>Esters</subject><subject>FATTY ACIDS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>IDENTIFICACION</subject><subject>IDENTIFICATION</subject><subject>Microbiology</subject><subject>New species</subject><subject>Phylogenetics</subject><subject>Physics, chemistry, biochemistry and biology of agricultural and forest soils</subject><subject>RHIZOBIUM</subject><subject>Root nodules</subject><subject>SIMBIOSIS</subject><subject>Soil science</subject><subject>Sphingobacteria</subject><subject>SYMBIOSE</subject><subject>SYMBIOSIS</subject><issn>0032-079X</issn><issn>1573-5036</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNpFkE1Lw0AQQBdRsFYvHgVhD-ItupvNfh21WBWKhaLgLUw2s7olTWI2OdRfb2oFT8PwHo9hCDnn7IYzpm_v5yzlRjAjDsiESy0SyYQ6JBPGRJowbd-PyUmMa7bbuZqQ5QraUNJQYt0HHxz0oalp4-nqM3w3RRg2NLboAkZaQMSSjtRhVQ0VdNRD328puDEANVTbGOIpOfJQRTz7m1PyNn94nT0li-Xj8-xukfhUZn2iEIW3iktnIFPokVkjbeql5qUxTnLkNiswBV16yUArK3khuC6dFciYFFNyve-2XfM1YOzzTYi7w6DGZog5l0YpbdJRvPoTITqofAe1CzFvu7CBbptnnHP727vca-vYN90_Tq2wmclGfrHnHpocProx8bKwko3vVuIH2bJvKg</recordid><startdate>19940401</startdate><enddate>19940401</enddate><creator>Jarvis, B.D.W. (Massey Univ., Palmerston North (New Zealand). Dept. of Microbiology and Genetics)</creator><creator>Tighe, S.W</creator><general>Kluwer Academic Publishers</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19940401</creationdate><title>Rapid identification of Rhizobium species based on cellular fatty acid analysis</title><author>Jarvis, B.D.W. (Massey Univ., Palmerston North (New Zealand). Dept. of Microbiology and Genetics) ; Tighe, S.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f254t-6ee3f9615c8a46efe098592f571d88c51e194be2a7df50a76951b317dc93e0053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>ACIDE GRAS</topic><topic>ACIDOS GRASOS</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Bacteria</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biochemistry and biology</topic><topic>Biological and medical sciences</topic><topic>Biological taxonomies</topic><topic>Chemical, physicochemical, biochemical and biological properties</topic><topic>Esters</topic><topic>FATTY ACIDS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>IDENTIFICACION</topic><topic>IDENTIFICATION</topic><topic>Microbiology</topic><topic>New species</topic><topic>Phylogenetics</topic><topic>Physics, chemistry, biochemistry and biology of agricultural and forest soils</topic><topic>RHIZOBIUM</topic><topic>Root nodules</topic><topic>SIMBIOSIS</topic><topic>Soil science</topic><topic>Sphingobacteria</topic><topic>SYMBIOSE</topic><topic>SYMBIOSIS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jarvis, B.D.W. (Massey Univ., Palmerston North (New Zealand). Dept. of Microbiology and Genetics)</creatorcontrib><creatorcontrib>Tighe, S.W</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant and soil</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jarvis, B.D.W. (Massey Univ., Palmerston North (New Zealand). Dept. of Microbiology and Genetics)</au><au>Tighe, S.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid identification of Rhizobium species based on cellular fatty acid analysis</atitle><jtitle>Plant and soil</jtitle><date>1994-04-01</date><risdate>1994</risdate><volume>161</volume><issue>1</issue><spage>31</spage><epage>41</epage><pages>31-41</pages><issn>0032-079X</issn><eissn>1573-5036</eissn><coden>PLSOA2</coden><abstract>As understanding of the evolutionary relationships between strains and species of root nodule bacteria increases the need for a rapid identification method that correlates well with phylogenetic relationships is clear. We have examined 123 strains of Rhizobium: R. fredii (19), R. galegae (20), R. leguminosarum (22), R. loti (17), R. meliloti (21), and R. tropici (18) and six unknowns. All strains were grown on modified tryptone yeast-extract (TY) agar, as log phase cultures, scraped from the agar, lysed, and the released fatty acids derivatized to their corresponding methyl esters. The methyl esters were analysed by gas-chromatography using the MIDI/Hewlett-Packard Microbial Identification System. All species studied contained 16:0, 17:0, 18:0 and 19cyclow9C fatty acids but only R loti and R tropici produced 12:0 3 OH, 13:0 iso 3 OH,18:1w9C and 15:0 iso 3 OH,17:0 iso 3 OH and 20:2w6,9C fatty acids respectively. Principal component analysis was used to show that strains could be divided into clusters corresponding to the six species. Fatty acid profiles for each species were developed and these correctly identified at least 95% of the strains belonging to each species. A dendrogram is presented showing the relationships between Rhizobium species based on fatty acid composition. The data base was used to identify unknown soil isolates as strains of Rhizobium lacking a symbiotic plasmid and a bacterium capable of expressing a symbiotic plasmid from R. leguminosarum as Sphingobacterium spiritovorum.</abstract><cop>Dordrecht</cop><pub>Kluwer Academic Publishers</pub><doi>10.1007/BF02183083</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 0032-079X |
| ispartof | Plant and soil, 1994-04, Vol.161 (1), p.31-41 |
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| language | eng |
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| subjects | ACIDE GRAS ACIDOS GRASOS Agronomy. Soil science and plant productions Bacteria Bacteriological methods and techniques used in bacteriology Bacteriology Biochemistry and biology Biological and medical sciences Biological taxonomies Chemical, physicochemical, biochemical and biological properties Esters FATTY ACIDS Fundamental and applied biological sciences. Psychology IDENTIFICACION IDENTIFICATION Microbiology New species Phylogenetics Physics, chemistry, biochemistry and biology of agricultural and forest soils RHIZOBIUM Root nodules SIMBIOSIS Soil science Sphingobacteria SYMBIOSE SYMBIOSIS |
| title | Rapid identification of Rhizobium species based on cellular fatty acid analysis |
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