Activation of ras oncogenes and expression of tumor‐specific transplantation antigens in methylcholanthrene‐induced murine fibrosarcomas
The DNA of 22 fibrosarcomas, newly induced in BALB/c mice by subcutaneous doses of 3‐methylcholanthrene (3‐MCA), was tested in NIH 3T3 transformation assay. Activation of K‐ras and N‐ras was found in 7 and 3 cases respectively. No H‐ras activation was detected. Polymerase chain reaction and oligonuc...
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Veröffentlicht in: | International journal of cancer 1991-02, Vol.47 (4), p.619-625 |
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description | The DNA of 22 fibrosarcomas, newly induced in BALB/c mice by subcutaneous doses of 3‐methylcholanthrene (3‐MCA), was tested in NIH 3T3 transformation assay. Activation of K‐ras and N‐ras was found in 7 and 3 cases respectively. No H‐ras activation was detected. Polymerase chain reaction and oligonucleotide hybridization performed on the DNA of the 22 sarcomas revealed 5 cases of K‐ras mutation at codon 12, 3 at codon 13 and I at both codons. One case of K13 mutation was not detectable by transfection. Three cases of mutation at codon 61 of N‐ras were also found, one of which was simultaneous with a K12 mutation. Tumor‐specific transplantation antigens (TSTA) were assessed in the 22 original tumors. Altogether 16 sarcomas were immunogenic, with the highest frequency of TSTA tumors (10/11 and 5/6) in the groups given 1.0 and 0.1 mg of 3‐MCA respectively, the lowest (1/5) in that with 0.01 mg of carcinogen; ras mutations occurred in the DNAs of 11 out of the 16 TSTA+ sarcomas, but none of the DNAs of the 6 TSTA‐ tumors showed ras mutation. The results suggest that 3‐MCA‐induced transformation of subcutaneous fibroblasts can involve mutations in codons 12, 13 or 61 of K‐ and N‐ but not H‐ras gene and that such mutation is accompanied by the expression of TSTA. |
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Activation of K‐ras and N‐ras was found in 7 and 3 cases respectively. No H‐ras activation was detected. Polymerase chain reaction and oligonucleotide hybridization performed on the DNA of the 22 sarcomas revealed 5 cases of K‐ras mutation at codon 12, 3 at codon 13 and I at both codons. One case of K13 mutation was not detectable by transfection. Three cases of mutation at codon 61 of N‐ras were also found, one of which was simultaneous with a K12 mutation. Tumor‐specific transplantation antigens (TSTA) were assessed in the 22 original tumors. Altogether 16 sarcomas were immunogenic, with the highest frequency of TSTA tumors (10/11 and 5/6) in the groups given 1.0 and 0.1 mg of 3‐MCA respectively, the lowest (1/5) in that with 0.01 mg of carcinogen; ras mutations occurred in the DNAs of 11 out of the 16 TSTA+ sarcomas, but none of the DNAs of the 6 TSTA‐ tumors showed ras mutation. The results suggest that 3‐MCA‐induced transformation of subcutaneous fibroblasts can involve mutations in codons 12, 13 or 61 of K‐ and N‐ but not H‐ras gene and that such mutation is accompanied by the expression of TSTA.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.2910470423</identifier><identifier>PMID: 1995490</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animal tumors. Experimental tumors ; Animals ; Antigens, Neoplasm - analysis ; Base Sequence ; Biological and medical sciences ; Experimental tumors, general aspects ; Female ; Fibrosarcoma - chemically induced ; Fibrosarcoma - genetics ; Fibrosarcoma - immunology ; Gene Expression Regulation ; Genes, ras ; Histocompatibility Antigens - analysis ; Medical sciences ; Methylcholanthrene ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Mutation ; Nucleic Acid Hybridization ; Transfection ; Tumors</subject><ispartof>International journal of cancer, 1991-02, Vol.47 (4), p.619-625</ispartof><rights>Copyright © 1991 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4013-277432d3e85456e649c2b5b3ae910772d0d909b3dbd2ee41498079f78ab265163</citedby><cites>FETCH-LOGICAL-c4013-277432d3e85456e649c2b5b3ae910772d0d909b3dbd2ee41498079f78ab265163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.2910470423$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.2910470423$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19853113$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1995490$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carbone, Giusi</creatorcontrib><creatorcontrib>Borrello, Maria Grazia</creatorcontrib><creatorcontrib>Molla, Alessandra</creatorcontrib><creatorcontrib>Rizzetti, Maria Grazia</creatorcontrib><creatorcontrib>Pierotti, Marco A.</creatorcontrib><creatorcontrib>Porta, Giuseppe Della</creatorcontrib><creatorcontrib>Parmiani, Giorgio</creatorcontrib><title>Activation of ras oncogenes and expression of tumor‐specific transplantation antigens in methylcholanthrene‐induced murine fibrosarcomas</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>The DNA of 22 fibrosarcomas, newly induced in BALB/c mice by subcutaneous doses of 3‐methylcholanthrene (3‐MCA), was tested in NIH 3T3 transformation assay. Activation of K‐ras and N‐ras was found in 7 and 3 cases respectively. No H‐ras activation was detected. Polymerase chain reaction and oligonucleotide hybridization performed on the DNA of the 22 sarcomas revealed 5 cases of K‐ras mutation at codon 12, 3 at codon 13 and I at both codons. One case of K13 mutation was not detectable by transfection. Three cases of mutation at codon 61 of N‐ras were also found, one of which was simultaneous with a K12 mutation. Tumor‐specific transplantation antigens (TSTA) were assessed in the 22 original tumors. Altogether 16 sarcomas were immunogenic, with the highest frequency of TSTA tumors (10/11 and 5/6) in the groups given 1.0 and 0.1 mg of 3‐MCA respectively, the lowest (1/5) in that with 0.01 mg of carcinogen; ras mutations occurred in the DNAs of 11 out of the 16 TSTA+ sarcomas, but none of the DNAs of the 6 TSTA‐ tumors showed ras mutation. The results suggest that 3‐MCA‐induced transformation of subcutaneous fibroblasts can involve mutations in codons 12, 13 or 61 of K‐ and N‐ but not H‐ras gene and that such mutation is accompanied by the expression of TSTA.</description><subject>Animal tumors. Experimental tumors</subject><subject>Animals</subject><subject>Antigens, Neoplasm - analysis</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Experimental tumors, general aspects</subject><subject>Female</subject><subject>Fibrosarcoma - chemically induced</subject><subject>Fibrosarcoma - genetics</subject><subject>Fibrosarcoma - immunology</subject><subject>Gene Expression Regulation</subject><subject>Genes, ras</subject><subject>Histocompatibility Antigens - analysis</subject><subject>Medical sciences</subject><subject>Methylcholanthrene</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Nucleic Acid Hybridization</subject><subject>Transfection</subject><subject>Tumors</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1v1DAYxy1EVY7CyobkBbYcfo3jsTpRKKrEUubIsZ9wrhI72Anltn4ABj4jnwSfclK7MfmR_i_-64fQG0q2lBD2wd_ZLdOUCEUE48_QhhKtKsKofI42xUAqRXn9Ar3M-Y4QSiUR5-icai2FJhv0-9LO_qeZfQw49jiZjGOw8TsEyNgEh-HXlCDnkz4vY0x_H_7kCazvvcVzMiFPgwnz2lEOX8IZ-4BHmPeHwe7jUd6nUlmSPrjFgsPjknwA3PsuxWySjaPJr9BZb4YMr0_vBfp29fF297m6-frpend5U1lBKK-YUoIzx6GRQtZQC21ZJztuoHBQijniNNEdd51jAIIK3RCle9WYjtWS1vwCvV97pxR_LJDndvTZwlB2QlxyS2VTM614MW5Xoy0jc4K-nZIfTTq0lLRH_G3B3z7iL4G3p-alG8E92lfeRX930k22ZugLPevzE1sjOaXHHr367v0Ah__82l5_2T3Z8A-_rqMy</recordid><startdate>19910220</startdate><enddate>19910220</enddate><creator>Carbone, Giusi</creator><creator>Borrello, Maria Grazia</creator><creator>Molla, Alessandra</creator><creator>Rizzetti, Maria Grazia</creator><creator>Pierotti, Marco A.</creator><creator>Porta, Giuseppe Della</creator><creator>Parmiani, Giorgio</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19910220</creationdate><title>Activation of ras oncogenes and expression of tumor‐specific transplantation antigens in methylcholanthrene‐induced murine fibrosarcomas</title><author>Carbone, Giusi ; Borrello, Maria Grazia ; Molla, Alessandra ; Rizzetti, Maria Grazia ; Pierotti, Marco A. ; Porta, Giuseppe Della ; Parmiani, Giorgio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4013-277432d3e85456e649c2b5b3ae910772d0d909b3dbd2ee41498079f78ab265163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Animal tumors. Experimental tumors</topic><topic>Animals</topic><topic>Antigens, Neoplasm - analysis</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Experimental tumors, general aspects</topic><topic>Female</topic><topic>Fibrosarcoma - chemically induced</topic><topic>Fibrosarcoma - genetics</topic><topic>Fibrosarcoma - immunology</topic><topic>Gene Expression Regulation</topic><topic>Genes, ras</topic><topic>Histocompatibility Antigens - analysis</topic><topic>Medical sciences</topic><topic>Methylcholanthrene</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Nucleic Acid Hybridization</topic><topic>Transfection</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carbone, Giusi</creatorcontrib><creatorcontrib>Borrello, Maria Grazia</creatorcontrib><creatorcontrib>Molla, Alessandra</creatorcontrib><creatorcontrib>Rizzetti, Maria Grazia</creatorcontrib><creatorcontrib>Pierotti, Marco A.</creatorcontrib><creatorcontrib>Porta, Giuseppe Della</creatorcontrib><creatorcontrib>Parmiani, Giorgio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carbone, Giusi</au><au>Borrello, Maria Grazia</au><au>Molla, Alessandra</au><au>Rizzetti, Maria Grazia</au><au>Pierotti, Marco A.</au><au>Porta, Giuseppe Della</au><au>Parmiani, Giorgio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation of ras oncogenes and expression of tumor‐specific transplantation antigens in methylcholanthrene‐induced murine fibrosarcomas</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>1991-02-20</date><risdate>1991</risdate><volume>47</volume><issue>4</issue><spage>619</spage><epage>625</epage><pages>619-625</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>The DNA of 22 fibrosarcomas, newly induced in BALB/c mice by subcutaneous doses of 3‐methylcholanthrene (3‐MCA), was tested in NIH 3T3 transformation assay. Activation of K‐ras and N‐ras was found in 7 and 3 cases respectively. No H‐ras activation was detected. Polymerase chain reaction and oligonucleotide hybridization performed on the DNA of the 22 sarcomas revealed 5 cases of K‐ras mutation at codon 12, 3 at codon 13 and I at both codons. One case of K13 mutation was not detectable by transfection. Three cases of mutation at codon 61 of N‐ras were also found, one of which was simultaneous with a K12 mutation. Tumor‐specific transplantation antigens (TSTA) were assessed in the 22 original tumors. Altogether 16 sarcomas were immunogenic, with the highest frequency of TSTA tumors (10/11 and 5/6) in the groups given 1.0 and 0.1 mg of 3‐MCA respectively, the lowest (1/5) in that with 0.01 mg of carcinogen; ras mutations occurred in the DNAs of 11 out of the 16 TSTA+ sarcomas, but none of the DNAs of the 6 TSTA‐ tumors showed ras mutation. The results suggest that 3‐MCA‐induced transformation of subcutaneous fibroblasts can involve mutations in codons 12, 13 or 61 of K‐ and N‐ but not H‐ras gene and that such mutation is accompanied by the expression of TSTA.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>1995490</pmid><doi>10.1002/ijc.2910470423</doi><tpages>7</tpages></addata></record> |
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subjects | Animal tumors. Experimental tumors Animals Antigens, Neoplasm - analysis Base Sequence Biological and medical sciences Experimental tumors, general aspects Female Fibrosarcoma - chemically induced Fibrosarcoma - genetics Fibrosarcoma - immunology Gene Expression Regulation Genes, ras Histocompatibility Antigens - analysis Medical sciences Methylcholanthrene Mice Mice, Inbred BALB C Molecular Sequence Data Mutation Nucleic Acid Hybridization Transfection Tumors |
title | Activation of ras oncogenes and expression of tumor‐specific transplantation antigens in methylcholanthrene‐induced murine fibrosarcomas |
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