Effects of Phyllanthus sellowianus Müll Arg. Extracts on the Rheological Properties of Human Erythrocytes
Phyllanthus sellowianus extracts have been used in Argentina since colonial times in the treatment of diabetes. The in vitro biorheological and hemoagglutinant action of different extracts of P. sellowianus bark on human erythrocytes (RBC) were studied. RBCs were incubated in vitro with four aqueous...
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| Veröffentlicht in: | Cell biochemistry and biophysics 2014-11, Vol.70 (2), p.1407-1416 |
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| creator | Buszniez, Patricia Di Sapio, Osvaldo Riquelme, Bibiana |
| description | Phyllanthus sellowianus
extracts have been used in Argentina since colonial times in the treatment of diabetes. The in vitro biorheological and hemoagglutinant action of different extracts of
P. sellowianus
bark on human erythrocytes (RBC) were studied. RBCs were incubated in vitro with four aqueous extracts: Maceration; Controlled Digestion (PD); Decoction; and Infusion. Biorheological parameters (deformability, membrane surface viscosity, elastic modulus, and dynamic viscolelasticity) were determined with an Erythrodeformeter, and erythrocyte adhesion was characterized by image digital analysis. Immunohematological assays in RBC incubated with all the extracts showed large globular aggregates and agglutination in human ABO blood group system. Isolated cell coefficient showed the increase of cell adhesion. Aggregated shape parameters were significantly higher than normal and they changed with the concentration, particularly of PD extracts. Rheological results showed that the extract biorheological action varies with the temperature used in the extract preparations. The results obtained are useful to study the action mechanism of extracts from
P. sellowianus
bark in order to evaluate its use as therapeutic agent in diabetes. Immunohematological Tests using ABO system showed its agglutinant power, which is of special interest in Immunohematology to be used as hemoclassifier. |
| doi_str_mv | 10.1007/s12013-014-0072-8 |
| format | Article |
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extracts have been used in Argentina since colonial times in the treatment of diabetes. The in vitro biorheological and hemoagglutinant action of different extracts of
P. sellowianus
bark on human erythrocytes (RBC) were studied. RBCs were incubated in vitro with four aqueous extracts: Maceration; Controlled Digestion (PD); Decoction; and Infusion. Biorheological parameters (deformability, membrane surface viscosity, elastic modulus, and dynamic viscolelasticity) were determined with an Erythrodeformeter, and erythrocyte adhesion was characterized by image digital analysis. Immunohematological assays in RBC incubated with all the extracts showed large globular aggregates and agglutination in human ABO blood group system. Isolated cell coefficient showed the increase of cell adhesion. Aggregated shape parameters were significantly higher than normal and they changed with the concentration, particularly of PD extracts. Rheological results showed that the extract biorheological action varies with the temperature used in the extract preparations. The results obtained are useful to study the action mechanism of extracts from
P. sellowianus
bark in order to evaluate its use as therapeutic agent in diabetes. Immunohematological Tests using ABO system showed its agglutinant power, which is of special interest in Immunohematology to be used as hemoclassifier.</description><identifier>ISSN: 1085-9195</identifier><identifier>EISSN: 1559-0283</identifier><identifier>DOI: 10.1007/s12013-014-0072-8</identifier><identifier>PMID: 24920190</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Adhesion ; Biochemistry ; Biological and Medical Physics ; Biomedical and Life Sciences ; Biophysics ; Biotechnology ; Cell Biology ; Erythrocyte Membrane - drug effects ; Erythrocytes ; Erythrocytes - cytology ; Erythrocytes - drug effects ; Erythrocytes - immunology ; Hemorheology - drug effects ; Humans ; Life Sciences ; Molecular Imaging ; Original Paper ; Pharmacology/Toxicology ; Phyllanthus - anatomy & histology ; Phyllanthus - chemistry ; Plant Bark - anatomy & histology ; Plant Bark - chemistry ; Plant Extracts - pharmacology</subject><ispartof>Cell biochemistry and biophysics, 2014-11, Vol.70 (2), p.1407-1416</ispartof><rights>Springer Science+Business Media New York 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-efca214319d44ffa9dd152e0837674f94abf0fc99e39bdc9497e94e3647f050b3</citedby><cites>FETCH-LOGICAL-c405t-efca214319d44ffa9dd152e0837674f94abf0fc99e39bdc9497e94e3647f050b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12013-014-0072-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12013-014-0072-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24920190$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Buszniez, Patricia</creatorcontrib><creatorcontrib>Di Sapio, Osvaldo</creatorcontrib><creatorcontrib>Riquelme, Bibiana</creatorcontrib><title>Effects of Phyllanthus sellowianus Müll Arg. Extracts on the Rheological Properties of Human Erythrocytes</title><title>Cell biochemistry and biophysics</title><addtitle>Cell Biochem Biophys</addtitle><addtitle>Cell Biochem Biophys</addtitle><description>Phyllanthus sellowianus
extracts have been used in Argentina since colonial times in the treatment of diabetes. The in vitro biorheological and hemoagglutinant action of different extracts of
P. sellowianus
bark on human erythrocytes (RBC) were studied. RBCs were incubated in vitro with four aqueous extracts: Maceration; Controlled Digestion (PD); Decoction; and Infusion. Biorheological parameters (deformability, membrane surface viscosity, elastic modulus, and dynamic viscolelasticity) were determined with an Erythrodeformeter, and erythrocyte adhesion was characterized by image digital analysis. Immunohematological assays in RBC incubated with all the extracts showed large globular aggregates and agglutination in human ABO blood group system. Isolated cell coefficient showed the increase of cell adhesion. Aggregated shape parameters were significantly higher than normal and they changed with the concentration, particularly of PD extracts. Rheological results showed that the extract biorheological action varies with the temperature used in the extract preparations. The results obtained are useful to study the action mechanism of extracts from
P. sellowianus
bark in order to evaluate its use as therapeutic agent in diabetes. Immunohematological Tests using ABO system showed its agglutinant power, which is of special interest in Immunohematology to be used as hemoclassifier.</description><subject>Adhesion</subject><subject>Biochemistry</subject><subject>Biological and Medical Physics</subject><subject>Biomedical and Life Sciences</subject><subject>Biophysics</subject><subject>Biotechnology</subject><subject>Cell Biology</subject><subject>Erythrocyte Membrane - drug effects</subject><subject>Erythrocytes</subject><subject>Erythrocytes - cytology</subject><subject>Erythrocytes - drug effects</subject><subject>Erythrocytes - immunology</subject><subject>Hemorheology - drug effects</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Molecular Imaging</subject><subject>Original Paper</subject><subject>Pharmacology/Toxicology</subject><subject>Phyllanthus - anatomy & histology</subject><subject>Phyllanthus - chemistry</subject><subject>Plant Bark - anatomy & histology</subject><subject>Plant Bark - chemistry</subject><subject>Plant Extracts - pharmacology</subject><issn>1085-9195</issn><issn>1559-0283</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kctuFDEQRS1ERELgA9ggS2zYdFJ-9MPLKBoSpCCiCNaWx12e7pGnPdhuhfk3dvkxPJmAEFJWLsvn3nLVJeQdgzMG0J4nxoGJCpisypVX3QtywupaVcA78bLU0NWVYqo-Jq9TWgNwDlK-IsdcqqJUcELWC-fQ5kSDo7fDznsz5WFONKH34X40U6m_PPzynl7E1Rld_MzRPOITzQPSuwGDD6vRGk9vY9hizCM-ml3PGzPRRdzlIQa7y5jekCNnfMK3T-cp-f5p8e3yurr5evX58uKmshLqXKGzhjMpmOqldM6ovmc1R-hE27TSKWmWDpxVCoVa9lZJ1aKSKBrZOqhhKU7Jx4PvNoYfM6asN2OyuB8Nw5w0q7sGVNuItqAf_kPXYY5T-V2hmqYVDZeiUOxA2RhSiuj0No4bE3eagd4HoQ9B6BKE3gehu6J5_-Q8LzfY_1X82XwB-AFI5WlaYfyn9bOuvwEVw5Qn</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Buszniez, Patricia</creator><creator>Di Sapio, Osvaldo</creator><creator>Riquelme, Bibiana</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20141101</creationdate><title>Effects of Phyllanthus sellowianus Müll Arg. Extracts on the Rheological Properties of Human Erythrocytes</title><author>Buszniez, Patricia ; Di Sapio, Osvaldo ; Riquelme, Bibiana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-efca214319d44ffa9dd152e0837674f94abf0fc99e39bdc9497e94e3647f050b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Adhesion</topic><topic>Biochemistry</topic><topic>Biological and Medical Physics</topic><topic>Biomedical and Life Sciences</topic><topic>Biophysics</topic><topic>Biotechnology</topic><topic>Cell Biology</topic><topic>Erythrocyte Membrane - drug effects</topic><topic>Erythrocytes</topic><topic>Erythrocytes - cytology</topic><topic>Erythrocytes - drug effects</topic><topic>Erythrocytes - immunology</topic><topic>Hemorheology - drug effects</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Molecular Imaging</topic><topic>Original Paper</topic><topic>Pharmacology/Toxicology</topic><topic>Phyllanthus - anatomy & histology</topic><topic>Phyllanthus - chemistry</topic><topic>Plant Bark - anatomy & histology</topic><topic>Plant Bark - chemistry</topic><topic>Plant Extracts - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Buszniez, Patricia</creatorcontrib><creatorcontrib>Di Sapio, Osvaldo</creatorcontrib><creatorcontrib>Riquelme, Bibiana</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buszniez, Patricia</au><au>Di Sapio, Osvaldo</au><au>Riquelme, Bibiana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Phyllanthus sellowianus Müll Arg. Extracts on the Rheological Properties of Human Erythrocytes</atitle><jtitle>Cell biochemistry and biophysics</jtitle><stitle>Cell Biochem Biophys</stitle><addtitle>Cell Biochem Biophys</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>70</volume><issue>2</issue><spage>1407</spage><epage>1416</epage><pages>1407-1416</pages><issn>1085-9195</issn><eissn>1559-0283</eissn><abstract>Phyllanthus sellowianus
extracts have been used in Argentina since colonial times in the treatment of diabetes. The in vitro biorheological and hemoagglutinant action of different extracts of
P. sellowianus
bark on human erythrocytes (RBC) were studied. RBCs were incubated in vitro with four aqueous extracts: Maceration; Controlled Digestion (PD); Decoction; and Infusion. Biorheological parameters (deformability, membrane surface viscosity, elastic modulus, and dynamic viscolelasticity) were determined with an Erythrodeformeter, and erythrocyte adhesion was characterized by image digital analysis. Immunohematological assays in RBC incubated with all the extracts showed large globular aggregates and agglutination in human ABO blood group system. Isolated cell coefficient showed the increase of cell adhesion. Aggregated shape parameters were significantly higher than normal and they changed with the concentration, particularly of PD extracts. Rheological results showed that the extract biorheological action varies with the temperature used in the extract preparations. The results obtained are useful to study the action mechanism of extracts from
P. sellowianus
bark in order to evaluate its use as therapeutic agent in diabetes. Immunohematological Tests using ABO system showed its agglutinant power, which is of special interest in Immunohematology to be used as hemoclassifier.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>24920190</pmid><doi>10.1007/s12013-014-0072-8</doi><tpages>10</tpages></addata></record> |
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| subjects | Adhesion Biochemistry Biological and Medical Physics Biomedical and Life Sciences Biophysics Biotechnology Cell Biology Erythrocyte Membrane - drug effects Erythrocytes Erythrocytes - cytology Erythrocytes - drug effects Erythrocytes - immunology Hemorheology - drug effects Humans Life Sciences Molecular Imaging Original Paper Pharmacology/Toxicology Phyllanthus - anatomy & histology Phyllanthus - chemistry Plant Bark - anatomy & histology Plant Bark - chemistry Plant Extracts - pharmacology |
| title | Effects of Phyllanthus sellowianus Müll Arg. Extracts on the Rheological Properties of Human Erythrocytes |
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