Fluorescence depolarization as an early measure of T lymphocyte stimulation

We have used the Cellscan, an apparatus capable of measuring optical properties of individual cells, to study changes in fluorescence polarization associated with T cell stimulation. We show that the fluorescence polarization of human peripheral blood lymphocytes (PBL) labeled with fluorescein diace...

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Veröffentlicht in:	Journal of immunological methods 1997-02, Vol.201 (1), p.15-24
Hauptverfasser:	Kaplan, Miriam R, Trubniykov, Ella, Berke, Gideon
Format:	Artikel
Sprache:	eng
Schlagworte:	1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine - pharmacology Cellscan Concanavalin A - pharmacology Cytometry Cytoplasm - ultrastructure Enzyme Inhibitors - pharmacology Fluorescein diacetate Fluoresceins Fluorescence Polarization Humans Lymphocyte Activation Lymphocyte Count Phosphatidylinositols - physiology Phytohemagglutinins - pharmacology Protein Kinase C - antagonists & inhibitors Staurosporine - pharmacology T cell stimulation T-Lymphocytes - physiology Tetradecanoylphorbol Acetate - pharmacology Time Factors
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