Reversible permeabilization of plasma membranes with an engineered switchable pore
By using an engineered, self-assembling, proteinaceous, 2-nm pore equipped with a metal-actuated switch, a technique to reversibly permeabilize the plasma membrane to small molecules (approximately 1000 Da) has been developed. We have demonstrated the dose-dependent permeabilization of fibroblasts b...
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| Veröffentlicht in: | Nature biotechnology 1997-03, Vol.15 (3), p.278-282 |
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| container_title | Nature biotechnology |
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| creator | RUSSO, M. J BAYLEY, H TONER, M |
| description | By using an engineered, self-assembling, proteinaceous, 2-nm pore equipped with a metal-actuated switch, a technique to reversibly permeabilize the plasma membrane to small molecules (approximately 1000 Da) has been developed. We have demonstrated the dose-dependent permeabilization of fibroblasts by pores designed to be blocked and unblocked by the addition and removal of microM concentrations of Zn2+. Further, we have shown that the activity of the switch allows permeabilized cells to maintain viability and ultrastructural integrity following the unconstrained flux of small molecules. This ability to control the transmembrane influx and efflux of molecules and thereby vary the intracellular environment yet maintain cell viability will impact an array of biological and medical problems. |
| doi_str_mv | 10.1038/nbt0397-278 |
| format | Article |
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This ability to control the transmembrane influx and efflux of molecules and thereby vary the intracellular environment yet maintain cell viability will impact an array of biological and medical problems.</description><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt0397-278</identifier><identifier>PMID: 9062930</identifier><identifier>CODEN: NABIF9</identifier><language>eng</language><publisher>New York, NY: Nature</publisher><subject>3T3 Cells ; Animals ; Bacterial Toxins - genetics ; Bacterial Toxins - pharmacology ; Biological and medical sciences ; Biological Transport ; Cell Membrane Permeability - drug effects ; Diverse techniques ; Fibroblasts - cytology ; Fibroblasts - metabolism ; Fibroblasts - ultrastructure ; Fundamental and applied biological sciences. 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This ability to control the transmembrane influx and efflux of molecules and thereby vary the intracellular environment yet maintain cell viability will impact an array of biological and medical problems.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacterial Toxins - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>Diverse techniques</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - metabolism</subject><subject>Fibroblasts - ultrastructure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hemolysin Proteins - genetics</subject><subject>Hemolysin Proteins - pharmacology</subject><subject>Mice</subject><subject>Microscopy, Electron</subject><subject>Molecular and cellular biology</subject><subject>Mutagenesis</subject><subject>Sucrose - metabolism</subject><subject>Zinc - pharmacology</subject><issn>1087-0156</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhoMoq66ePAs5iBepZto0SY-y-AULwqLnkqYTN9Ivk66iv97olj3NMO_Dy_AQcgbsGlimbrpqZFkhk1SqPXIEORcJiELsx50pmTDIxSE5DuGdMSa4EDMyK5hIi4wdkdUKP9EHVzVIB_Qt6so17kePru9ob-nQ6NBq2mJbed1hoF9uXFPdUezeXIfosaYh3sxa_1f0Hk_IgdVNwNNpzsnr_d3L4jFZPj88LW6XickUG5NKmbo2vLZKKl4XiqOSoBkoCyC0BQ2VkTItFEsryLjOrQJhrcgtogbOszm53PYOvv_YYBjL1gWDTRP_7DehhFzlmZAigldb0Pg-BI-2HLxrtf8ugZV_BsvJYBkNRvp8qt1ULdY7dlIW84sp18HoxkYtxoUdlooUhBLZL7lHedA</recordid><startdate>19970301</startdate><enddate>19970301</enddate><creator>RUSSO, M. 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Psychology</topic><topic>Hemolysin Proteins - genetics</topic><topic>Hemolysin Proteins - pharmacology</topic><topic>Mice</topic><topic>Microscopy, Electron</topic><topic>Molecular and cellular biology</topic><topic>Mutagenesis</topic><topic>Sucrose - metabolism</topic><topic>Zinc - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RUSSO, M. 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| fulltext | fulltext |
| identifier | ISSN: 1087-0156 |
| ispartof | Nature biotechnology, 1997-03, Vol.15 (3), p.278-282 |
| issn | 1087-0156 1546-1696 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | 3T3 Cells Animals Bacterial Toxins - genetics Bacterial Toxins - pharmacology Biological and medical sciences Biological Transport Cell Membrane Permeability - drug effects Diverse techniques Fibroblasts - cytology Fibroblasts - metabolism Fibroblasts - ultrastructure Fundamental and applied biological sciences. Psychology Hemolysin Proteins - genetics Hemolysin Proteins - pharmacology Mice Microscopy, Electron Molecular and cellular biology Mutagenesis Sucrose - metabolism Zinc - pharmacology |
| title | Reversible permeabilization of plasma membranes with an engineered switchable pore |
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