Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression
Expression of chalcone synthase (CHS), the first enzyme in the flavonoid branch of the phenylpropanoid biosynthetic pathway in plants, is induced by developmental cues and environmental stimuli. We used plant transformation technology to delineate the functional structure of the French bean CHS15 ge...
Gespeichert in:
Veröffentlicht in: | Plant molecular biology 1996-12, Vol.32 (5), p.849-859 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 859 |
---|---|
container_issue | 5 |
container_start_page | 849 |
container_title | Plant molecular biology |
container_volume | 32 |
creator | Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.) Kooter, J.M Dixon, R.A Lamb, C.J |
description | Expression of chalcone synthase (CHS), the first enzyme in the flavonoid branch of the phenylpropanoid biosynthetic pathway in plants, is induced by developmental cues and environmental stimuli. We used plant transformation technology to delineate the functional structure of the French bean CHS15 gene promoter during plant development. In the absence of an efficient transformation procedure for bean, Nicotiana tabacum was used as the model plant. CHS15 promoter activity, evaluated by measurements of beta-D-glucuronidase (GUS) activity, revealed a tissue-specific pattern of expression similar to that reported for CHS genes in bean. GUS activity was observed in flowers and root tips. Floral expression was confined to the pigmented part of petals and was induced in a transient fashion. Fine mapping of promoter cis-elements was accomplished using a set of promoter mutants generated by unidirectional deletions or by site-directed mutagenesis. Maximal floral and root-specific expression was found to require sequence elements located on both sides of the TATA-box. Two adjacent sequence motifs, the G-box (CACGTG) and H-box (CCTACC(N)7CT) located near the TATA-box, were both essential for floral expression, and were also found to be important for root-specific expression. The CHS15 promoter is regulated by a complex interplay between different cis-elements and their cognate factors. The conservation of both the G-box and H-box in different CHS promoters emphasizes their importance as regulatory motifs. |
doi_str_mv | 10.1007/bf00020482 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_15853438</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15853438</sourcerecordid><originalsourceid>FETCH-LOGICAL-c398t-344f904994221debd93928159fb8d37c71a8474b874484eab030ec99245f3bc73</originalsourceid><addsrcrecordid>eNo9UU1vGyEQRVWr1Pm49FipEqccKm0DC2vg2EZxWslqLsl5BeyQUK3BBRw1_yM_ONPazWmYeW_mDfMI-cDZF86YunCBMdYzqfs3ZMEHJbqB9fotWTC-VJ2UvH9Pjmv9xRjSxfKIHGmj2SCWC_K82iXfYk52plOsFf4lNAdqqQObqH-ws88JaH1K7cFWoPeA2bbkTW5QaEy0FZsqVqOnLTvrfabb2aZWaYFHsHOlFX7vIHmg2BNDpYBCqUXUDLnQMOeCT_izLQig_Cl5F7ANzg7xhNytrm4vv3frm-sfl1_XnRdGt05IGQyTxsi-5xO4yQjTaz6Y4PQklFfcaqmk00pKLcE6Jhh4Y3o5BOG8EifkfD8Xf4ML1jZuYvUw4_KQd3Xkgx6EFBqJn_dEX3KtBcK4LXFjy9PI2fjXgvHb6r8FSP50mLpzG5heqYebI_5xjwebR3tfYh1_ro1Cx5gUL7oei-c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15853438</pqid></control><display><type>article</type><title>Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.) ; Kooter, J.M ; Dixon, R.A ; Lamb, C.J</creator><creatorcontrib>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.) ; Kooter, J.M ; Dixon, R.A ; Lamb, C.J</creatorcontrib><description>Expression of chalcone synthase (CHS), the first enzyme in the flavonoid branch of the phenylpropanoid biosynthetic pathway in plants, is induced by developmental cues and environmental stimuli. We used plant transformation technology to delineate the functional structure of the French bean CHS15 gene promoter during plant development. In the absence of an efficient transformation procedure for bean, Nicotiana tabacum was used as the model plant. CHS15 promoter activity, evaluated by measurements of beta-D-glucuronidase (GUS) activity, revealed a tissue-specific pattern of expression similar to that reported for CHS genes in bean. GUS activity was observed in flowers and root tips. Floral expression was confined to the pigmented part of petals and was induced in a transient fashion. Fine mapping of promoter cis-elements was accomplished using a set of promoter mutants generated by unidirectional deletions or by site-directed mutagenesis. Maximal floral and root-specific expression was found to require sequence elements located on both sides of the TATA-box. Two adjacent sequence motifs, the G-box (CACGTG) and H-box (CCTACC(N)7CT) located near the TATA-box, were both essential for floral expression, and were also found to be important for root-specific expression. The CHS15 promoter is regulated by a complex interplay between different cis-elements and their cognate factors. The conservation of both the G-box and H-box in different CHS promoters emphasizes their importance as regulatory motifs.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/bf00020482</identifier><identifier>PMID: 8980536</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Acyltransferases - genetics ; Base Sequence ; CALCONA ; CHALCONE ; CHALCONES ; DNA, Plant ; ENZIMAS ; ENZYME ; ENZYMES ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fabaceae - enzymology ; Fabaceae - genetics ; Fabaceae - physiology ; FLAVONOIDE ; FLAVONOIDES ; FLAVONOIDS ; FLORACION ; FLORAISON ; FLOWERING ; GENE EXPRESSION ; Glucuronidase - genetics ; Molecular Sequence Data ; Nicotiana - genetics ; Nicotiana - physiology ; NICOTIANA TABACUM ; Phaseolus vulgaris ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants, Genetically Modified ; Plants, Medicinal ; Plants, Toxic ; Point Mutation ; Promoter Regions, Genetic ; Recombinant Fusion Proteins - genetics ; TRANSGENIC PLANTS</subject><ispartof>Plant molecular biology, 1996-12, Vol.32 (5), p.849-859</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-344f904994221debd93928159fb8d37c71a8474b874484eab030ec99245f3bc73</citedby><cites>FETCH-LOGICAL-c398t-344f904994221debd93928159fb8d37c71a8474b874484eab030ec99245f3bc73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8980536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.)</creatorcontrib><creatorcontrib>Kooter, J.M</creatorcontrib><creatorcontrib>Dixon, R.A</creatorcontrib><creatorcontrib>Lamb, C.J</creatorcontrib><title>Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Expression of chalcone synthase (CHS), the first enzyme in the flavonoid branch of the phenylpropanoid biosynthetic pathway in plants, is induced by developmental cues and environmental stimuli. We used plant transformation technology to delineate the functional structure of the French bean CHS15 gene promoter during plant development. In the absence of an efficient transformation procedure for bean, Nicotiana tabacum was used as the model plant. CHS15 promoter activity, evaluated by measurements of beta-D-glucuronidase (GUS) activity, revealed a tissue-specific pattern of expression similar to that reported for CHS genes in bean. GUS activity was observed in flowers and root tips. Floral expression was confined to the pigmented part of petals and was induced in a transient fashion. Fine mapping of promoter cis-elements was accomplished using a set of promoter mutants generated by unidirectional deletions or by site-directed mutagenesis. Maximal floral and root-specific expression was found to require sequence elements located on both sides of the TATA-box. Two adjacent sequence motifs, the G-box (CACGTG) and H-box (CCTACC(N)7CT) located near the TATA-box, were both essential for floral expression, and were also found to be important for root-specific expression. The CHS15 promoter is regulated by a complex interplay between different cis-elements and their cognate factors. The conservation of both the G-box and H-box in different CHS promoters emphasizes their importance as regulatory motifs.</description><subject>Acyltransferases - genetics</subject><subject>Base Sequence</subject><subject>CALCONA</subject><subject>CHALCONE</subject><subject>CHALCONES</subject><subject>DNA, Plant</subject><subject>ENZIMAS</subject><subject>ENZYME</subject><subject>ENZYMES</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fabaceae - enzymology</subject><subject>Fabaceae - genetics</subject><subject>Fabaceae - physiology</subject><subject>FLAVONOIDE</subject><subject>FLAVONOIDES</subject><subject>FLAVONOIDS</subject><subject>FLORACION</subject><subject>FLORAISON</subject><subject>FLOWERING</subject><subject>GENE EXPRESSION</subject><subject>Glucuronidase - genetics</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - physiology</subject><subject>NICOTIANA TABACUM</subject><subject>Phaseolus vulgaris</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants, Genetically Modified</subject><subject>Plants, Medicinal</subject><subject>Plants, Toxic</subject><subject>Point Mutation</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>TRANSGENIC PLANTS</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UU1vGyEQRVWr1Pm49FipEqccKm0DC2vg2EZxWslqLsl5BeyQUK3BBRw1_yM_ONPazWmYeW_mDfMI-cDZF86YunCBMdYzqfs3ZMEHJbqB9fotWTC-VJ2UvH9Pjmv9xRjSxfKIHGmj2SCWC_K82iXfYk52plOsFf4lNAdqqQObqH-ws88JaH1K7cFWoPeA2bbkTW5QaEy0FZsqVqOnLTvrfabb2aZWaYFHsHOlFX7vIHmg2BNDpYBCqUXUDLnQMOeCT_izLQig_Cl5F7ANzg7xhNytrm4vv3frm-sfl1_XnRdGt05IGQyTxsi-5xO4yQjTaz6Y4PQklFfcaqmk00pKLcE6Jhh4Y3o5BOG8EifkfD8Xf4ML1jZuYvUw4_KQd3Xkgx6EFBqJn_dEX3KtBcK4LXFjy9PI2fjXgvHb6r8FSP50mLpzG5heqYebI_5xjwebR3tfYh1_ro1Cx5gUL7oei-c</recordid><startdate>19961201</startdate><enddate>19961201</enddate><creator>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.)</creator><creator>Kooter, J.M</creator><creator>Dixon, R.A</creator><creator>Lamb, C.J</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19961201</creationdate><title>Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression</title><author>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.) ; Kooter, J.M ; Dixon, R.A ; Lamb, C.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-344f904994221debd93928159fb8d37c71a8474b874484eab030ec99245f3bc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Acyltransferases - genetics</topic><topic>Base Sequence</topic><topic>CALCONA</topic><topic>CHALCONE</topic><topic>CHALCONES</topic><topic>DNA, Plant</topic><topic>ENZIMAS</topic><topic>ENZYME</topic><topic>ENZYMES</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fabaceae - enzymology</topic><topic>Fabaceae - genetics</topic><topic>Fabaceae - physiology</topic><topic>FLAVONOIDE</topic><topic>FLAVONOIDES</topic><topic>FLAVONOIDS</topic><topic>FLORACION</topic><topic>FLORAISON</topic><topic>FLOWERING</topic><topic>GENE EXPRESSION</topic><topic>Glucuronidase - genetics</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - physiology</topic><topic>NICOTIANA TABACUM</topic><topic>Phaseolus vulgaris</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants, Genetically Modified</topic><topic>Plants, Medicinal</topic><topic>Plants, Toxic</topic><topic>Point Mutation</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>TRANSGENIC PLANTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.)</creatorcontrib><creatorcontrib>Kooter, J.M</creatorcontrib><creatorcontrib>Dixon, R.A</creatorcontrib><creatorcontrib>Lamb, C.J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.)</au><au>Kooter, J.M</au><au>Dixon, R.A</au><au>Lamb, C.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1996-12-01</date><risdate>1996</risdate><volume>32</volume><issue>5</issue><spage>849</spage><epage>859</epage><pages>849-859</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Expression of chalcone synthase (CHS), the first enzyme in the flavonoid branch of the phenylpropanoid biosynthetic pathway in plants, is induced by developmental cues and environmental stimuli. We used plant transformation technology to delineate the functional structure of the French bean CHS15 gene promoter during plant development. In the absence of an efficient transformation procedure for bean, Nicotiana tabacum was used as the model plant. CHS15 promoter activity, evaluated by measurements of beta-D-glucuronidase (GUS) activity, revealed a tissue-specific pattern of expression similar to that reported for CHS genes in bean. GUS activity was observed in flowers and root tips. Floral expression was confined to the pigmented part of petals and was induced in a transient fashion. Fine mapping of promoter cis-elements was accomplished using a set of promoter mutants generated by unidirectional deletions or by site-directed mutagenesis. Maximal floral and root-specific expression was found to require sequence elements located on both sides of the TATA-box. Two adjacent sequence motifs, the G-box (CACGTG) and H-box (CCTACC(N)7CT) located near the TATA-box, were both essential for floral expression, and were also found to be important for root-specific expression. The CHS15 promoter is regulated by a complex interplay between different cis-elements and their cognate factors. The conservation of both the G-box and H-box in different CHS promoters emphasizes their importance as regulatory motifs.</abstract><cop>Netherlands</cop><pmid>8980536</pmid><doi>10.1007/bf00020482</doi><tpages>11</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0167-4412 |
ispartof | Plant molecular biology, 1996-12, Vol.32 (5), p.849-859 |
issn | 0167-4412 1573-5028 |
language | eng |
recordid | cdi_proquest_miscellaneous_15853438 |
source | MEDLINE; Springer Nature - Complete Springer Journals |
subjects | Acyltransferases - genetics Base Sequence CALCONA CHALCONE CHALCONES DNA, Plant ENZIMAS ENZYME ENZYMES EXPRESION GENICA EXPRESSION DES GENES Fabaceae - enzymology Fabaceae - genetics Fabaceae - physiology FLAVONOIDE FLAVONOIDES FLAVONOIDS FLORACION FLORAISON FLOWERING GENE EXPRESSION Glucuronidase - genetics Molecular Sequence Data Nicotiana - genetics Nicotiana - physiology NICOTIANA TABACUM Phaseolus vulgaris PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants, Genetically Modified Plants, Medicinal Plants, Toxic Point Mutation Promoter Regions, Genetic Recombinant Fusion Proteins - genetics TRANSGENIC PLANTS |
title | Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T05%3A08%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20dissection%20of%20a%20bean%20chalcone%20synthase%20gene%20promoter%20in%20transgenic%20tobacco%20plants%20reveals%20sequence%20motifs%20essential%20for%20floral%20expression&rft.jtitle=Plant%20molecular%20biology&rft.au=Faktor,%20O.%20(Salk%20Inst.%20for%20Biological%20Studies,%20La%20Jolla,%20CA%20(USA).%20Plant%20Biology%20Lab.)&rft.date=1996-12-01&rft.volume=32&rft.issue=5&rft.spage=849&rft.epage=859&rft.pages=849-859&rft.issn=0167-4412&rft.eissn=1573-5028&rft_id=info:doi/10.1007/bf00020482&rft_dat=%3Cproquest_cross%3E15853438%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15853438&rft_id=info:pmid/8980536&rfr_iscdi=true |