Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression

Expression of chalcone synthase (CHS), the first enzyme in the flavonoid branch of the phenylpropanoid biosynthetic pathway in plants, is induced by developmental cues and environmental stimuli. We used plant transformation technology to delineate the functional structure of the French bean CHS15 ge...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Plant molecular biology 1996-12, Vol.32 (5), p.849-859
Hauptverfasser: Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.), Kooter, J.M, Dixon, R.A, Lamb, C.J
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 859
container_issue 5
container_start_page 849
container_title Plant molecular biology
container_volume 32
creator Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.)
Kooter, J.M
Dixon, R.A
Lamb, C.J
description Expression of chalcone synthase (CHS), the first enzyme in the flavonoid branch of the phenylpropanoid biosynthetic pathway in plants, is induced by developmental cues and environmental stimuli. We used plant transformation technology to delineate the functional structure of the French bean CHS15 gene promoter during plant development. In the absence of an efficient transformation procedure for bean, Nicotiana tabacum was used as the model plant. CHS15 promoter activity, evaluated by measurements of beta-D-glucuronidase (GUS) activity, revealed a tissue-specific pattern of expression similar to that reported for CHS genes in bean. GUS activity was observed in flowers and root tips. Floral expression was confined to the pigmented part of petals and was induced in a transient fashion. Fine mapping of promoter cis-elements was accomplished using a set of promoter mutants generated by unidirectional deletions or by site-directed mutagenesis. Maximal floral and root-specific expression was found to require sequence elements located on both sides of the TATA-box. Two adjacent sequence motifs, the G-box (CACGTG) and H-box (CCTACC(N)7CT) located near the TATA-box, were both essential for floral expression, and were also found to be important for root-specific expression. The CHS15 promoter is regulated by a complex interplay between different cis-elements and their cognate factors. The conservation of both the G-box and H-box in different CHS promoters emphasizes their importance as regulatory motifs.
doi_str_mv 10.1007/bf00020482
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_15853438</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15853438</sourcerecordid><originalsourceid>FETCH-LOGICAL-c398t-344f904994221debd93928159fb8d37c71a8474b874484eab030ec99245f3bc73</originalsourceid><addsrcrecordid>eNo9UU1vGyEQRVWr1Pm49FipEqccKm0DC2vg2EZxWslqLsl5BeyQUK3BBRw1_yM_ONPazWmYeW_mDfMI-cDZF86YunCBMdYzqfs3ZMEHJbqB9fotWTC-VJ2UvH9Pjmv9xRjSxfKIHGmj2SCWC_K82iXfYk52plOsFf4lNAdqqQObqH-ws88JaH1K7cFWoPeA2bbkTW5QaEy0FZsqVqOnLTvrfabb2aZWaYFHsHOlFX7vIHmg2BNDpYBCqUXUDLnQMOeCT_izLQig_Cl5F7ANzg7xhNytrm4vv3frm-sfl1_XnRdGt05IGQyTxsi-5xO4yQjTaz6Y4PQklFfcaqmk00pKLcE6Jhh4Y3o5BOG8EifkfD8Xf4ML1jZuYvUw4_KQd3Xkgx6EFBqJn_dEX3KtBcK4LXFjy9PI2fjXgvHb6r8FSP50mLpzG5heqYebI_5xjwebR3tfYh1_ro1Cx5gUL7oei-c</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15853438</pqid></control><display><type>article</type><title>Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.) ; Kooter, J.M ; Dixon, R.A ; Lamb, C.J</creator><creatorcontrib>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.) ; Kooter, J.M ; Dixon, R.A ; Lamb, C.J</creatorcontrib><description>Expression of chalcone synthase (CHS), the first enzyme in the flavonoid branch of the phenylpropanoid biosynthetic pathway in plants, is induced by developmental cues and environmental stimuli. We used plant transformation technology to delineate the functional structure of the French bean CHS15 gene promoter during plant development. In the absence of an efficient transformation procedure for bean, Nicotiana tabacum was used as the model plant. CHS15 promoter activity, evaluated by measurements of beta-D-glucuronidase (GUS) activity, revealed a tissue-specific pattern of expression similar to that reported for CHS genes in bean. GUS activity was observed in flowers and root tips. Floral expression was confined to the pigmented part of petals and was induced in a transient fashion. Fine mapping of promoter cis-elements was accomplished using a set of promoter mutants generated by unidirectional deletions or by site-directed mutagenesis. Maximal floral and root-specific expression was found to require sequence elements located on both sides of the TATA-box. Two adjacent sequence motifs, the G-box (CACGTG) and H-box (CCTACC(N)7CT) located near the TATA-box, were both essential for floral expression, and were also found to be important for root-specific expression. The CHS15 promoter is regulated by a complex interplay between different cis-elements and their cognate factors. The conservation of both the G-box and H-box in different CHS promoters emphasizes their importance as regulatory motifs.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/bf00020482</identifier><identifier>PMID: 8980536</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Acyltransferases - genetics ; Base Sequence ; CALCONA ; CHALCONE ; CHALCONES ; DNA, Plant ; ENZIMAS ; ENZYME ; ENZYMES ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fabaceae - enzymology ; Fabaceae - genetics ; Fabaceae - physiology ; FLAVONOIDE ; FLAVONOIDES ; FLAVONOIDS ; FLORACION ; FLORAISON ; FLOWERING ; GENE EXPRESSION ; Glucuronidase - genetics ; Molecular Sequence Data ; Nicotiana - genetics ; Nicotiana - physiology ; NICOTIANA TABACUM ; Phaseolus vulgaris ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants, Genetically Modified ; Plants, Medicinal ; Plants, Toxic ; Point Mutation ; Promoter Regions, Genetic ; Recombinant Fusion Proteins - genetics ; TRANSGENIC PLANTS</subject><ispartof>Plant molecular biology, 1996-12, Vol.32 (5), p.849-859</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-344f904994221debd93928159fb8d37c71a8474b874484eab030ec99245f3bc73</citedby><cites>FETCH-LOGICAL-c398t-344f904994221debd93928159fb8d37c71a8474b874484eab030ec99245f3bc73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8980536$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.)</creatorcontrib><creatorcontrib>Kooter, J.M</creatorcontrib><creatorcontrib>Dixon, R.A</creatorcontrib><creatorcontrib>Lamb, C.J</creatorcontrib><title>Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Expression of chalcone synthase (CHS), the first enzyme in the flavonoid branch of the phenylpropanoid biosynthetic pathway in plants, is induced by developmental cues and environmental stimuli. We used plant transformation technology to delineate the functional structure of the French bean CHS15 gene promoter during plant development. In the absence of an efficient transformation procedure for bean, Nicotiana tabacum was used as the model plant. CHS15 promoter activity, evaluated by measurements of beta-D-glucuronidase (GUS) activity, revealed a tissue-specific pattern of expression similar to that reported for CHS genes in bean. GUS activity was observed in flowers and root tips. Floral expression was confined to the pigmented part of petals and was induced in a transient fashion. Fine mapping of promoter cis-elements was accomplished using a set of promoter mutants generated by unidirectional deletions or by site-directed mutagenesis. Maximal floral and root-specific expression was found to require sequence elements located on both sides of the TATA-box. Two adjacent sequence motifs, the G-box (CACGTG) and H-box (CCTACC(N)7CT) located near the TATA-box, were both essential for floral expression, and were also found to be important for root-specific expression. The CHS15 promoter is regulated by a complex interplay between different cis-elements and their cognate factors. The conservation of both the G-box and H-box in different CHS promoters emphasizes their importance as regulatory motifs.</description><subject>Acyltransferases - genetics</subject><subject>Base Sequence</subject><subject>CALCONA</subject><subject>CHALCONE</subject><subject>CHALCONES</subject><subject>DNA, Plant</subject><subject>ENZIMAS</subject><subject>ENZYME</subject><subject>ENZYMES</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fabaceae - enzymology</subject><subject>Fabaceae - genetics</subject><subject>Fabaceae - physiology</subject><subject>FLAVONOIDE</subject><subject>FLAVONOIDES</subject><subject>FLAVONOIDS</subject><subject>FLORACION</subject><subject>FLORAISON</subject><subject>FLOWERING</subject><subject>GENE EXPRESSION</subject><subject>Glucuronidase - genetics</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - physiology</subject><subject>NICOTIANA TABACUM</subject><subject>Phaseolus vulgaris</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants, Genetically Modified</subject><subject>Plants, Medicinal</subject><subject>Plants, Toxic</subject><subject>Point Mutation</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>TRANSGENIC PLANTS</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UU1vGyEQRVWr1Pm49FipEqccKm0DC2vg2EZxWslqLsl5BeyQUK3BBRw1_yM_ONPazWmYeW_mDfMI-cDZF86YunCBMdYzqfs3ZMEHJbqB9fotWTC-VJ2UvH9Pjmv9xRjSxfKIHGmj2SCWC_K82iXfYk52plOsFf4lNAdqqQObqH-ws88JaH1K7cFWoPeA2bbkTW5QaEy0FZsqVqOnLTvrfabb2aZWaYFHsHOlFX7vIHmg2BNDpYBCqUXUDLnQMOeCT_izLQig_Cl5F7ANzg7xhNytrm4vv3frm-sfl1_XnRdGt05IGQyTxsi-5xO4yQjTaz6Y4PQklFfcaqmk00pKLcE6Jhh4Y3o5BOG8EifkfD8Xf4ML1jZuYvUw4_KQd3Xkgx6EFBqJn_dEX3KtBcK4LXFjy9PI2fjXgvHb6r8FSP50mLpzG5heqYebI_5xjwebR3tfYh1_ro1Cx5gUL7oei-c</recordid><startdate>19961201</startdate><enddate>19961201</enddate><creator>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.)</creator><creator>Kooter, J.M</creator><creator>Dixon, R.A</creator><creator>Lamb, C.J</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19961201</creationdate><title>Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression</title><author>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.) ; Kooter, J.M ; Dixon, R.A ; Lamb, C.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-344f904994221debd93928159fb8d37c71a8474b874484eab030ec99245f3bc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Acyltransferases - genetics</topic><topic>Base Sequence</topic><topic>CALCONA</topic><topic>CHALCONE</topic><topic>CHALCONES</topic><topic>DNA, Plant</topic><topic>ENZIMAS</topic><topic>ENZYME</topic><topic>ENZYMES</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fabaceae - enzymology</topic><topic>Fabaceae - genetics</topic><topic>Fabaceae - physiology</topic><topic>FLAVONOIDE</topic><topic>FLAVONOIDES</topic><topic>FLAVONOIDS</topic><topic>FLORACION</topic><topic>FLORAISON</topic><topic>FLOWERING</topic><topic>GENE EXPRESSION</topic><topic>Glucuronidase - genetics</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - physiology</topic><topic>NICOTIANA TABACUM</topic><topic>Phaseolus vulgaris</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants, Genetically Modified</topic><topic>Plants, Medicinal</topic><topic>Plants, Toxic</topic><topic>Point Mutation</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>TRANSGENIC PLANTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.)</creatorcontrib><creatorcontrib>Kooter, J.M</creatorcontrib><creatorcontrib>Dixon, R.A</creatorcontrib><creatorcontrib>Lamb, C.J</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Faktor, O. (Salk Inst. for Biological Studies, La Jolla, CA (USA). Plant Biology Lab.)</au><au>Kooter, J.M</au><au>Dixon, R.A</au><au>Lamb, C.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1996-12-01</date><risdate>1996</risdate><volume>32</volume><issue>5</issue><spage>849</spage><epage>859</epage><pages>849-859</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Expression of chalcone synthase (CHS), the first enzyme in the flavonoid branch of the phenylpropanoid biosynthetic pathway in plants, is induced by developmental cues and environmental stimuli. We used plant transformation technology to delineate the functional structure of the French bean CHS15 gene promoter during plant development. In the absence of an efficient transformation procedure for bean, Nicotiana tabacum was used as the model plant. CHS15 promoter activity, evaluated by measurements of beta-D-glucuronidase (GUS) activity, revealed a tissue-specific pattern of expression similar to that reported for CHS genes in bean. GUS activity was observed in flowers and root tips. Floral expression was confined to the pigmented part of petals and was induced in a transient fashion. Fine mapping of promoter cis-elements was accomplished using a set of promoter mutants generated by unidirectional deletions or by site-directed mutagenesis. Maximal floral and root-specific expression was found to require sequence elements located on both sides of the TATA-box. Two adjacent sequence motifs, the G-box (CACGTG) and H-box (CCTACC(N)7CT) located near the TATA-box, were both essential for floral expression, and were also found to be important for root-specific expression. The CHS15 promoter is regulated by a complex interplay between different cis-elements and their cognate factors. The conservation of both the G-box and H-box in different CHS promoters emphasizes their importance as regulatory motifs.</abstract><cop>Netherlands</cop><pmid>8980536</pmid><doi>10.1007/bf00020482</doi><tpages>11</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0167-4412
ispartof Plant molecular biology, 1996-12, Vol.32 (5), p.849-859
issn 0167-4412
1573-5028
language eng
recordid cdi_proquest_miscellaneous_15853438
source MEDLINE; Springer Nature - Complete Springer Journals
subjects Acyltransferases - genetics
Base Sequence
CALCONA
CHALCONE
CHALCONES
DNA, Plant
ENZIMAS
ENZYME
ENZYMES
EXPRESION GENICA
EXPRESSION DES GENES
Fabaceae - enzymology
Fabaceae - genetics
Fabaceae - physiology
FLAVONOIDE
FLAVONOIDES
FLAVONOIDS
FLORACION
FLORAISON
FLOWERING
GENE EXPRESSION
Glucuronidase - genetics
Molecular Sequence Data
Nicotiana - genetics
Nicotiana - physiology
NICOTIANA TABACUM
Phaseolus vulgaris
PLANTAS TRANSGENICAS
PLANTE TRANSGENIQUE
Plants, Genetically Modified
Plants, Medicinal
Plants, Toxic
Point Mutation
Promoter Regions, Genetic
Recombinant Fusion Proteins - genetics
TRANSGENIC PLANTS
title Functional dissection of a bean chalcone synthase gene promoter in transgenic tobacco plants reveals sequence motifs essential for floral expression
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T05%3A08%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Functional%20dissection%20of%20a%20bean%20chalcone%20synthase%20gene%20promoter%20in%20transgenic%20tobacco%20plants%20reveals%20sequence%20motifs%20essential%20for%20floral%20expression&rft.jtitle=Plant%20molecular%20biology&rft.au=Faktor,%20O.%20(Salk%20Inst.%20for%20Biological%20Studies,%20La%20Jolla,%20CA%20(USA).%20Plant%20Biology%20Lab.)&rft.date=1996-12-01&rft.volume=32&rft.issue=5&rft.spage=849&rft.epage=859&rft.pages=849-859&rft.issn=0167-4412&rft.eissn=1573-5028&rft_id=info:doi/10.1007/bf00020482&rft_dat=%3Cproquest_cross%3E15853438%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15853438&rft_id=info:pmid/8980536&rfr_iscdi=true