Serum amyloid P component: its role in platelet activation stimulated by sphingomyelinase D purified from the venom of the brown recluse spider (Loxosceles reclusa)
C. A. Gates and R. S. Rees. Serum amyloid P component: its role in platelet activation stimulated by sphingomyelinase D purified from the venom of the brown recluse spider ( Loxosceles reclusa). Toxicon 28, 1303–1315, 1990.—Serum amyloid P component or serum amyloid protein is a ubiquitous, highly c...
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creator | Gates, Caryl A. Rees, Riley S. |
description | C. A.
Gates and R. S.
Rees. Serum amyloid P component: its role in platelet activation stimulated by sphingomyelinase D purified from the venom of the brown recluse spider (
Loxosceles reclusa).
Toxicon
28, 1303–1315, 1990.—Serum amyloid P component or serum amyloid protein is a ubiquitous, highly conserved glycoprotein whose function is unknown. Although the related pentraxin, C-reactive protein, is an acute phase reactant in man, there is no direct evidence that human serum amyloid protein is involved in an inflammatory response. Here we show that serum amyloid protein is required by sphingomyelinase D, the principal necrotic agent of the venom of
Loxosceles reclusa, for the in vitro-activation of human platelets. Furthermore, this platelet activation is dependent upon the presence of only serum amyloid protein; no other plasma components are necessary. Secretion of [
3H]-serotonin and aggregation of platelets are nearly maximal following incubation of the platelets with purified sphingomyelinase D (0.3 μg/ml) and 5 μg/ml pure serum amyloid protein in the presence of calcium. Since the platelets are no longer activated when this 10% physiologic amount of serum amyloid protein is omitted, serum amyloid protein is likely to have a role in the necrosis caused by brown recluse spider venom. |
doi_str_mv | 10.1016/0041-0101(90)90095-O |
format | Article |
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Gates and R. S.
Rees. Serum amyloid P component: its role in platelet activation stimulated by sphingomyelinase D purified from the venom of the brown recluse spider (
Loxosceles reclusa).
Toxicon
28, 1303–1315, 1990.—Serum amyloid P component or serum amyloid protein is a ubiquitous, highly conserved glycoprotein whose function is unknown. Although the related pentraxin, C-reactive protein, is an acute phase reactant in man, there is no direct evidence that human serum amyloid protein is involved in an inflammatory response. Here we show that serum amyloid protein is required by sphingomyelinase D, the principal necrotic agent of the venom of
Loxosceles reclusa, for the in vitro-activation of human platelets. Furthermore, this platelet activation is dependent upon the presence of only serum amyloid protein; no other plasma components are necessary. Secretion of [
3H]-serotonin and aggregation of platelets are nearly maximal following incubation of the platelets with purified sphingomyelinase D (0.3 μg/ml) and 5 μg/ml pure serum amyloid protein in the presence of calcium. Since the platelets are no longer activated when this 10% physiologic amount of serum amyloid protein is omitted, serum amyloid protein is likely to have a role in the necrosis caused by brown recluse spider venom.</description><identifier>ISSN: 0041-0101</identifier><identifier>EISSN: 1879-3150</identifier><identifier>DOI: 10.1016/0041-0101(90)90095-O</identifier><identifier>PMID: 1965062</identifier><identifier>CODEN: TOXIA6</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animal poisons toxicology. Antivenoms ; Animals ; Araneidae ; Biological and medical sciences ; Buffers ; C-Reactive Protein - isolation & purification ; C-Reactive Protein - physiology ; Dose-Response Relationship, Drug ; Fetal Blood - drug effects ; Fetal Blood - physiology ; Humans ; Loxoscelidae ; man ; Medical sciences ; Phosphoric Diester Hydrolases - isolation & purification ; Phosphoric Diester Hydrolases - pharmacology ; Platelet Activation - drug effects ; platelets ; Serum Amyloid P-Component - isolation & purification ; Serum Amyloid P-Component - physiology ; sphingomyelinase D ; Spider Venoms - chemistry ; Spider Venoms - toxicity ; Toxicology ; venom</subject><ispartof>Toxicon (Oxford), 1990, Vol.28 (11), p.1303-1315</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-fd9752beccb1d963df20051d3f4f799d30b2d06a4ee96504a9ea7b521b1bd6843</citedby><cites>FETCH-LOGICAL-c418t-fd9752beccb1d963df20051d3f4f799d30b2d06a4ee96504a9ea7b521b1bd6843</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0041-0101(90)90095-O$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19422986$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1965062$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gates, Caryl A.</creatorcontrib><creatorcontrib>Rees, Riley S.</creatorcontrib><title>Serum amyloid P component: its role in platelet activation stimulated by sphingomyelinase D purified from the venom of the brown recluse spider (Loxosceles reclusa)</title><title>Toxicon (Oxford)</title><addtitle>Toxicon</addtitle><description>C. A.
Gates and R. S.
Rees. Serum amyloid P component: its role in platelet activation stimulated by sphingomyelinase D purified from the venom of the brown recluse spider (
Loxosceles reclusa).
Toxicon
28, 1303–1315, 1990.—Serum amyloid P component or serum amyloid protein is a ubiquitous, highly conserved glycoprotein whose function is unknown. Although the related pentraxin, C-reactive protein, is an acute phase reactant in man, there is no direct evidence that human serum amyloid protein is involved in an inflammatory response. Here we show that serum amyloid protein is required by sphingomyelinase D, the principal necrotic agent of the venom of
Loxosceles reclusa, for the in vitro-activation of human platelets. Furthermore, this platelet activation is dependent upon the presence of only serum amyloid protein; no other plasma components are necessary. Secretion of [
3H]-serotonin and aggregation of platelets are nearly maximal following incubation of the platelets with purified sphingomyelinase D (0.3 μg/ml) and 5 μg/ml pure serum amyloid protein in the presence of calcium. Since the platelets are no longer activated when this 10% physiologic amount of serum amyloid protein is omitted, serum amyloid protein is likely to have a role in the necrosis caused by brown recluse spider venom.</description><subject>Animal poisons toxicology. Antivenoms</subject><subject>Animals</subject><subject>Araneidae</subject><subject>Biological and medical sciences</subject><subject>Buffers</subject><subject>C-Reactive Protein - isolation & purification</subject><subject>C-Reactive Protein - physiology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fetal Blood - drug effects</subject><subject>Fetal Blood - physiology</subject><subject>Humans</subject><subject>Loxoscelidae</subject><subject>man</subject><subject>Medical sciences</subject><subject>Phosphoric Diester Hydrolases - isolation & purification</subject><subject>Phosphoric Diester Hydrolases - pharmacology</subject><subject>Platelet Activation - drug effects</subject><subject>platelets</subject><subject>Serum Amyloid P-Component - isolation & purification</subject><subject>Serum Amyloid P-Component - physiology</subject><subject>sphingomyelinase D</subject><subject>Spider Venoms - chemistry</subject><subject>Spider Venoms - toxicity</subject><subject>Toxicology</subject><subject>venom</subject><issn>0041-0101</issn><issn>1879-3150</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU2PFCEQJUazjqP_QBMumt1Da9Ff03gwMetnMsmYqGdCQ-FiuqEX6Nmd_-MPld6ZqCdPFLxXVY_3CHnK4CUD1r4CqFkBuTzncMEBeFPs7pEV6za8qFgD98nqD-UheRTjTwCoOt6ekTPG2wbackV-fcUwj1SOh8FbTb9Q5cfJO3TpNbUp0uAHpNbRaZAJB0xUqmT3MlnvaEx2nJd3TfsDjdOVdT_8eMDBOhmRvqPTHKyxGTbBjzRdId2jy5U3d5c--BtHA6phzvQ4WY2Bnm_9rY8q74onSF48Jg-MHCI-OZ1r8v3D-2-Xn4rt7uPny7fbQtWsS4XRfNOUPSrVM83bSpsSoGG6MrXZcK4r6EsNrawRl-_XkqPc9E3JetbrtqurNXlxnDsFfz1jTGK0i5RBOvRzFKzpqq6tWCbWR6IKPsaARkzBjjIcBAOxhCMW58XivOAg7sIRu9z27DR_7kfUf5uOaWT8-QmXUcnBBOmUjf_Q6rLkWcCavDnyMJuxtxhEVBadQm2zZUlob_8v5Ddiaa7H</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Gates, Caryl A.</creator><creator>Rees, Riley S.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7SS</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M81</scope><scope>P64</scope></search><sort><creationdate>1990</creationdate><title>Serum amyloid P component: its role in platelet activation stimulated by sphingomyelinase D purified from the venom of the brown recluse spider (Loxosceles reclusa)</title><author>Gates, Caryl A. ; Rees, Riley S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-fd9752beccb1d963df20051d3f4f799d30b2d06a4ee96504a9ea7b521b1bd6843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animal poisons toxicology. Antivenoms</topic><topic>Animals</topic><topic>Araneidae</topic><topic>Biological and medical sciences</topic><topic>Buffers</topic><topic>C-Reactive Protein - isolation & purification</topic><topic>C-Reactive Protein - physiology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fetal Blood - drug effects</topic><topic>Fetal Blood - physiology</topic><topic>Humans</topic><topic>Loxoscelidae</topic><topic>man</topic><topic>Medical sciences</topic><topic>Phosphoric Diester Hydrolases - isolation & purification</topic><topic>Phosphoric Diester Hydrolases - pharmacology</topic><topic>Platelet Activation - drug effects</topic><topic>platelets</topic><topic>Serum Amyloid P-Component - isolation & purification</topic><topic>Serum Amyloid P-Component - physiology</topic><topic>sphingomyelinase D</topic><topic>Spider Venoms - chemistry</topic><topic>Spider Venoms - toxicity</topic><topic>Toxicology</topic><topic>venom</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gates, Caryl A.</creatorcontrib><creatorcontrib>Rees, Riley S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 3</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Toxicon (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gates, Caryl A.</au><au>Rees, Riley S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Serum amyloid P component: its role in platelet activation stimulated by sphingomyelinase D purified from the venom of the brown recluse spider (Loxosceles reclusa)</atitle><jtitle>Toxicon (Oxford)</jtitle><addtitle>Toxicon</addtitle><date>1990</date><risdate>1990</risdate><volume>28</volume><issue>11</issue><spage>1303</spage><epage>1315</epage><pages>1303-1315</pages><issn>0041-0101</issn><eissn>1879-3150</eissn><coden>TOXIA6</coden><abstract>C. A.
Gates and R. S.
Rees. Serum amyloid P component: its role in platelet activation stimulated by sphingomyelinase D purified from the venom of the brown recluse spider (
Loxosceles reclusa).
Toxicon
28, 1303–1315, 1990.—Serum amyloid P component or serum amyloid protein is a ubiquitous, highly conserved glycoprotein whose function is unknown. Although the related pentraxin, C-reactive protein, is an acute phase reactant in man, there is no direct evidence that human serum amyloid protein is involved in an inflammatory response. Here we show that serum amyloid protein is required by sphingomyelinase D, the principal necrotic agent of the venom of
Loxosceles reclusa, for the in vitro-activation of human platelets. Furthermore, this platelet activation is dependent upon the presence of only serum amyloid protein; no other plasma components are necessary. Secretion of [
3H]-serotonin and aggregation of platelets are nearly maximal following incubation of the platelets with purified sphingomyelinase D (0.3 μg/ml) and 5 μg/ml pure serum amyloid protein in the presence of calcium. Since the platelets are no longer activated when this 10% physiologic amount of serum amyloid protein is omitted, serum amyloid protein is likely to have a role in the necrosis caused by brown recluse spider venom.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>1965062</pmid><doi>10.1016/0041-0101(90)90095-O</doi><tpages>13</tpages></addata></record> |
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subjects | Animal poisons toxicology. Antivenoms Animals Araneidae Biological and medical sciences Buffers C-Reactive Protein - isolation & purification C-Reactive Protein - physiology Dose-Response Relationship, Drug Fetal Blood - drug effects Fetal Blood - physiology Humans Loxoscelidae man Medical sciences Phosphoric Diester Hydrolases - isolation & purification Phosphoric Diester Hydrolases - pharmacology Platelet Activation - drug effects platelets Serum Amyloid P-Component - isolation & purification Serum Amyloid P-Component - physiology sphingomyelinase D Spider Venoms - chemistry Spider Venoms - toxicity Toxicology venom |
title | Serum amyloid P component: its role in platelet activation stimulated by sphingomyelinase D purified from the venom of the brown recluse spider (Loxosceles reclusa) |
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