Purification and immunochemical detection of β-naphthoflavone-and phenobarbital-induced avian cytochrome P450 enzymes
Livers from mallards (Anas platyrhynchos) were treated with either β‐naphthoflavone (50 mg/kg) or phenobarbital (70 mg/kg). Purification of induced hepatic cytochrome P450 was accomplished using both DEAE and hydroxyapatite columns, as well as sodium dodecyl sulfate polyacrylamide gel electrophoresi...
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| Veröffentlicht in: | Environmental toxicology and chemistry 1996-12, Vol.15 (12), p.2293-2298 |
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| container_title | Environmental toxicology and chemistry |
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| creator | Brown, Randal L. Levi, Patricia E. Hodgson, Ernest Melancon, Mark J. |
| description | Livers from mallards (Anas platyrhynchos) were treated with either β‐naphthoflavone (50 mg/kg) or phenobarbital (70 mg/kg). Purification of induced hepatic cytochrome P450 was accomplished using both DEAE and hydroxyapatite columns, as well as sodium dodecyl sulfate polyacrylamide gel electrophoresis separation. Polyclonal antibodies to these proteins were then produced in young male New Zealand White rabbits. β‐Naphthoflavone (βNF)‐ and phenobarbital (PB)‐treated red‐winged blackbird, screech owl, European starling, and lesser scaup liver microsomes were analyzed in western blots for species cross‐reactivity. Although all four of these avian species exhibited cross‐reactivity with antibodies to βNF‐induced mallard P450, all but the lesser scaup revealed a protein of higher molecular weight than that of the βNF‐induced mallard. In addition, only the lesser scaup exhibited cross‐reactivity with the anti‐PB‐induced mallard P450 antibodies. |
| doi_str_mv | 10.1002/etc.5620151226 |
| format | Article |
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Purification of induced hepatic cytochrome P450 was accomplished using both DEAE and hydroxyapatite columns, as well as sodium dodecyl sulfate polyacrylamide gel electrophoresis separation. Polyclonal antibodies to these proteins were then produced in young male New Zealand White rabbits. β‐Naphthoflavone (βNF)‐ and phenobarbital (PB)‐treated red‐winged blackbird, screech owl, European starling, and lesser scaup liver microsomes were analyzed in western blots for species cross‐reactivity. Although all four of these avian species exhibited cross‐reactivity with antibodies to βNF‐induced mallard P450, all but the lesser scaup revealed a protein of higher molecular weight than that of the βNF‐induced mallard. In addition, only the lesser scaup exhibited cross‐reactivity with the anti‐PB‐induced mallard P450 antibodies.</description><identifier>ISSN: 0730-7268</identifier><identifier>EISSN: 1552-8618</identifier><identifier>DOI: 10.1002/etc.5620151226</identifier><identifier>CODEN: ETOCDK</identifier><language>eng</language><publisher>Hoboken: Wiley Periodicals, Inc</publisher><subject>Anas platyrhynchos ; Animal, plant and microbial ecology ; Applied ecology ; Aves ; Biological and medical sciences ; Biomarker ; Cytochrome P450 ; Ecotoxicology, biological effects of pollution ; Effects of pollution and side effects of pesticides on vertebrates ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Phenobarbital ; β-Naphthoflavone</subject><ispartof>Environmental toxicology and chemistry, 1996-12, Vol.15 (12), p.2293-2298</ispartof><rights>Copyright © 1996 SETAC</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4166-aebe268293f0c7cf2f0c382840d69821f301a71b1a81ea261447602cab817f523</citedby><cites>FETCH-LOGICAL-c4166-aebe268293f0c7cf2f0c382840d69821f301a71b1a81ea261447602cab817f523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fetc.5620151226$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fetc.5620151226$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2523874$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Brown, Randal L.</creatorcontrib><creatorcontrib>Levi, Patricia E.</creatorcontrib><creatorcontrib>Hodgson, Ernest</creatorcontrib><creatorcontrib>Melancon, Mark J.</creatorcontrib><title>Purification and immunochemical detection of β-naphthoflavone-and phenobarbital-induced avian cytochrome P450 enzymes</title><title>Environmental toxicology and chemistry</title><addtitle>Environmental Toxicology and Chemistry</addtitle><description>Livers from mallards (Anas platyrhynchos) were treated with either β‐naphthoflavone (50 mg/kg) or phenobarbital (70 mg/kg). Purification of induced hepatic cytochrome P450 was accomplished using both DEAE and hydroxyapatite columns, as well as sodium dodecyl sulfate polyacrylamide gel electrophoresis separation. Polyclonal antibodies to these proteins were then produced in young male New Zealand White rabbits. β‐Naphthoflavone (βNF)‐ and phenobarbital (PB)‐treated red‐winged blackbird, screech owl, European starling, and lesser scaup liver microsomes were analyzed in western blots for species cross‐reactivity. Although all four of these avian species exhibited cross‐reactivity with antibodies to βNF‐induced mallard P450, all but the lesser scaup revealed a protein of higher molecular weight than that of the βNF‐induced mallard. In addition, only the lesser scaup exhibited cross‐reactivity with the anti‐PB‐induced mallard P450 antibodies.</description><subject>Anas platyrhynchos</subject><subject>Animal, plant and microbial ecology</subject><subject>Applied ecology</subject><subject>Aves</subject><subject>Biological and medical sciences</subject><subject>Biomarker</subject><subject>Cytochrome P450</subject><subject>Ecotoxicology, biological effects of pollution</subject><subject>Effects of pollution and side effects of pesticides on vertebrates</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Phenobarbital</subject><subject>β-Naphthoflavone</subject><issn>0730-7268</issn><issn>1552-8618</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkc9u1DAQxiMEEkvLlXMOiJu3tpPYzhGtoC0qdPknjtbEGWsNib3YycL2sfogPBNutyri1LmMNP5934z8FcULRpeMUn6Ck1k2glPWMM7Fo2LBmoYTJZh6XCyorCiRXKinxbOUvlPKRNu2i2K3nqOzzsDkgi_B96Ubx9kHs8ExT4eyxwnN7WOw5Z9r4mG7mTbBDrALHsmNYrtBHzqInZtgIM73s8G-hJ0DX5r9lL1iGLFc1w0t0V_tR0zHxRMLQ8Lnd_2o-Pr2zZfVGbm4PD1fvb4gpmZCEMAO8828rSw10lieW6W4qmkvWsWZrSgDyToGiiFwwepaCsoNdIpJ2_DqqHh18N3G8HPGNOnRJYPDAB7DnDRrVEWVEg-DdS4u2wwuD6CJIaWIVm-jGyHuNaP6Jgedc9D_csiCl3fOkPKH2gjeuHSv4vlKJeuMtQfslxtw_4CpzuR_K8hB69KEv--1EH9oISvZ6G8fTvXZu_Xq88f1e_2p-guBd6oA</recordid><startdate>199612</startdate><enddate>199612</enddate><creator>Brown, Randal L.</creator><creator>Levi, Patricia E.</creator><creator>Hodgson, Ernest</creator><creator>Melancon, Mark J.</creator><general>Wiley Periodicals, Inc</general><general>SETAC</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope><scope>7TV</scope><scope>7U7</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>199612</creationdate><title>Purification and immunochemical detection of β-naphthoflavone-and phenobarbital-induced avian cytochrome P450 enzymes</title><author>Brown, Randal L. ; Levi, Patricia E. ; Hodgson, Ernest ; Melancon, Mark J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4166-aebe268293f0c7cf2f0c382840d69821f301a71b1a81ea261447602cab817f523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Anas platyrhynchos</topic><topic>Animal, plant and microbial ecology</topic><topic>Applied ecology</topic><topic>Aves</topic><topic>Biological and medical sciences</topic><topic>Biomarker</topic><topic>Cytochrome P450</topic><topic>Ecotoxicology, biological effects of pollution</topic><topic>Effects of pollution and side effects of pesticides on vertebrates</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Phenobarbital</topic><topic>β-Naphthoflavone</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brown, Randal L.</creatorcontrib><creatorcontrib>Levi, Patricia E.</creatorcontrib><creatorcontrib>Hodgson, Ernest</creatorcontrib><creatorcontrib>Melancon, Mark J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Environmental toxicology and chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brown, Randal L.</au><au>Levi, Patricia E.</au><au>Hodgson, Ernest</au><au>Melancon, Mark J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification and immunochemical detection of β-naphthoflavone-and phenobarbital-induced avian cytochrome P450 enzymes</atitle><jtitle>Environmental toxicology and chemistry</jtitle><addtitle>Environmental Toxicology and Chemistry</addtitle><date>1996-12</date><risdate>1996</risdate><volume>15</volume><issue>12</issue><spage>2293</spage><epage>2298</epage><pages>2293-2298</pages><issn>0730-7268</issn><eissn>1552-8618</eissn><coden>ETOCDK</coden><abstract>Livers from mallards (Anas platyrhynchos) were treated with either β‐naphthoflavone (50 mg/kg) or phenobarbital (70 mg/kg). Purification of induced hepatic cytochrome P450 was accomplished using both DEAE and hydroxyapatite columns, as well as sodium dodecyl sulfate polyacrylamide gel electrophoresis separation. Polyclonal antibodies to these proteins were then produced in young male New Zealand White rabbits. β‐Naphthoflavone (βNF)‐ and phenobarbital (PB)‐treated red‐winged blackbird, screech owl, European starling, and lesser scaup liver microsomes were analyzed in western blots for species cross‐reactivity. Although all four of these avian species exhibited cross‐reactivity with antibodies to βNF‐induced mallard P450, all but the lesser scaup revealed a protein of higher molecular weight than that of the βNF‐induced mallard. In addition, only the lesser scaup exhibited cross‐reactivity with the anti‐PB‐induced mallard P450 antibodies.</abstract><cop>Hoboken</cop><pub>Wiley Periodicals, Inc</pub><doi>10.1002/etc.5620151226</doi><tpages>6</tpages></addata></record> |
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| ispartof | Environmental toxicology and chemistry, 1996-12, Vol.15 (12), p.2293-2298 |
| issn | 0730-7268 1552-8618 |
| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Anas platyrhynchos Animal, plant and microbial ecology Applied ecology Aves Biological and medical sciences Biomarker Cytochrome P450 Ecotoxicology, biological effects of pollution Effects of pollution and side effects of pesticides on vertebrates Freshwater Fundamental and applied biological sciences. Psychology Phenobarbital β-Naphthoflavone |
| title | Purification and immunochemical detection of β-naphthoflavone-and phenobarbital-induced avian cytochrome P450 enzymes |
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