Mechanism of inactivation of tobacco mosaic virus with ozone
The inactivation mechanism of tobacco mosaic virus (TMV) in a phosphate buffer (pH 6.9) by ozone was studied. We previously reported that the damage of naked TMV-RNA occurred at the guanine moiety of RNA (Shinriki et al., Biochim. biophys. Acta 655, 323, 1981). In this paper, we clarified the mode o...
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| Veröffentlicht in: | Water research (Oxford) 1988, Vol.22 (7), p.933-938 |
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| creator | Shinriki, Nariko Ishizaki, Kozo Yoshizaki, Toshimichi Miura, Kazunobu Ueda, Tohru |
| description | The inactivation mechanism of tobacco mosaic virus (TMV) in a phosphate buffer (pH 6.9) by ozone was studied. We previously reported that the damage of naked TMV-RNA occurred at the guanine moiety of RNA (Shinriki
et al., Biochim. biophys. Acta
655, 323, 1981). In this paper, we clarified the mode of the inactivation of TMV by using tritium-labeled TMV (TMV
∗) prepared by the reconstitution of tritium-labeled TMV-RNA (TMV-RNA
∗) and coat protein of TMV. It was found that the amount of extracted TMV-RNA
∗ from ozone-treated TMV
∗ decreased with the advance of ozonization, and that there was good correlation between the loss of infectivity and the decrease of recovery of TMV-RNA
∗. When TMV lost its infectivity due to ozone, tryptophan and tyrosine of the coat protein were also degraded by ozone. Polyacrylamide gel electrophoretic analysis of the substance produced during ozonization showed that the coat protein subunits were aggregated with each other and cross-linked with TMV-RNA
∗. From these results, it was concluded that the inability of uncoating is the major cause of the TMV inactivation by ozone. |
| doi_str_mv | 10.1016/0043-1354(88)90031-0 |
| format | Article |
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et al., Biochim. biophys. Acta
655, 323, 1981). In this paper, we clarified the mode of the inactivation of TMV by using tritium-labeled TMV (TMV
∗) prepared by the reconstitution of tritium-labeled TMV-RNA (TMV-RNA
∗) and coat protein of TMV. It was found that the amount of extracted TMV-RNA
∗ from ozone-treated TMV
∗ decreased with the advance of ozonization, and that there was good correlation between the loss of infectivity and the decrease of recovery of TMV-RNA
∗. When TMV lost its infectivity due to ozone, tryptophan and tyrosine of the coat protein were also degraded by ozone. Polyacrylamide gel electrophoretic analysis of the substance produced during ozonization showed that the coat protein subunits were aggregated with each other and cross-linked with TMV-RNA
∗. From these results, it was concluded that the inability of uncoating is the major cause of the TMV inactivation by ozone.</description><identifier>ISSN: 0043-1354</identifier><identifier>EISSN: 1879-2448</identifier><identifier>DOI: 10.1016/0043-1354(88)90031-0</identifier><identifier>CODEN: WATRAG</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Action of physical and chemical agents ; Biological and medical sciences ; degradation ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control ; inactivation ; infectivity ; Microbiology ; ozone ; ozonization ; Phytopathology. Animal pests. Plant and forest protection ; Plant viruses and viroids ; protein coat ; RNA ; tobacco mosaic virus ; tryptophan ; tyrosine ; Virology</subject><ispartof>Water research (Oxford), 1988, Vol.22 (7), p.933-938</ispartof><rights>1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-e2dceb2ac3fa4af77e9e86693d2f371c7018edf1481b245d558c1a722f88802f3</citedby><cites>FETCH-LOGICAL-c395t-e2dceb2ac3fa4af77e9e86693d2f371c7018edf1481b245d558c1a722f88802f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0043135488900310$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7046512$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Shinriki, Nariko</creatorcontrib><creatorcontrib>Ishizaki, Kozo</creatorcontrib><creatorcontrib>Yoshizaki, Toshimichi</creatorcontrib><creatorcontrib>Miura, Kazunobu</creatorcontrib><creatorcontrib>Ueda, Tohru</creatorcontrib><title>Mechanism of inactivation of tobacco mosaic virus with ozone</title><title>Water research (Oxford)</title><description>The inactivation mechanism of tobacco mosaic virus (TMV) in a phosphate buffer (pH 6.9) by ozone was studied. We previously reported that the damage of naked TMV-RNA occurred at the guanine moiety of RNA (Shinriki
et al., Biochim. biophys. Acta
655, 323, 1981). In this paper, we clarified the mode of the inactivation of TMV by using tritium-labeled TMV (TMV
∗) prepared by the reconstitution of tritium-labeled TMV-RNA (TMV-RNA
∗) and coat protein of TMV. It was found that the amount of extracted TMV-RNA
∗ from ozone-treated TMV
∗ decreased with the advance of ozonization, and that there was good correlation between the loss of infectivity and the decrease of recovery of TMV-RNA
∗. When TMV lost its infectivity due to ozone, tryptophan and tyrosine of the coat protein were also degraded by ozone. Polyacrylamide gel electrophoretic analysis of the substance produced during ozonization showed that the coat protein subunits were aggregated with each other and cross-linked with TMV-RNA
∗. From these results, it was concluded that the inability of uncoating is the major cause of the TMV inactivation by ozone.</description><subject>Action of physical and chemical agents</subject><subject>Biological and medical sciences</subject><subject>degradation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</subject><subject>inactivation</subject><subject>infectivity</subject><subject>Microbiology</subject><subject>ozone</subject><subject>ozonization</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant viruses and viroids</subject><subject>protein coat</subject><subject>RNA</subject><subject>tobacco mosaic virus</subject><subject>tryptophan</subject><subject>tyrosine</subject><subject>Virology</subject><issn>0043-1354</issn><issn>1879-2448</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNqFkEtLw0AUhQdRsFb_gYssRHQRnWcyARGk-IKKG10P05s7dKTJ1Jm0or_exEqXuroc-M658BFyzOgFo6y4pFSKnAklz7Q-rygVLKc7ZMR0WeVcSr1LRltknxyk9EYp5VxUI3L1hDC3rU9NFlzmWwudX9vOh3bIXZhZgJA1IVkP2drHVco-fDfPwldo8ZDsObtIePR7x-T17vZl8pBPn-8fJzfTHESluhx5DTjjFoSz0rqyxAp1UVSi5k6UDErKNNaOSc1mXKpaKQ3Mlpw7rTXtmTE53ewuY3hfYepM4xPgYmFbDKtkmNKcV4X8HxRlIVShelBuQIghpYjOLKNvbPw0jJrBqRmEmUGY0dr8ODW0r5387tsEduGibcGnbbekslCM99j1BsNeytpjNAk8toC1jwidqYP_-883U_mJiA</recordid><startdate>1988</startdate><enddate>1988</enddate><creator>Shinriki, Nariko</creator><creator>Ishizaki, Kozo</creator><creator>Yoshizaki, Toshimichi</creator><creator>Miura, Kazunobu</creator><creator>Ueda, Tohru</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7TV</scope><scope>C1K</scope></search><sort><creationdate>1988</creationdate><title>Mechanism of inactivation of tobacco mosaic virus with ozone</title><author>Shinriki, Nariko ; Ishizaki, Kozo ; Yoshizaki, Toshimichi ; Miura, Kazunobu ; Ueda, Tohru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-e2dceb2ac3fa4af77e9e86693d2f371c7018edf1481b245d558c1a722f88802f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Action of physical and chemical agents</topic><topic>Biological and medical sciences</topic><topic>degradation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</topic><topic>inactivation</topic><topic>infectivity</topic><topic>Microbiology</topic><topic>ozone</topic><topic>ozonization</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant viruses and viroids</topic><topic>protein coat</topic><topic>RNA</topic><topic>tobacco mosaic virus</topic><topic>tryptophan</topic><topic>tyrosine</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shinriki, Nariko</creatorcontrib><creatorcontrib>Ishizaki, Kozo</creatorcontrib><creatorcontrib>Yoshizaki, Toshimichi</creatorcontrib><creatorcontrib>Miura, Kazunobu</creatorcontrib><creatorcontrib>Ueda, Tohru</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Pollution Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Water research (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shinriki, Nariko</au><au>Ishizaki, Kozo</au><au>Yoshizaki, Toshimichi</au><au>Miura, Kazunobu</au><au>Ueda, Tohru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanism of inactivation of tobacco mosaic virus with ozone</atitle><jtitle>Water research (Oxford)</jtitle><date>1988</date><risdate>1988</risdate><volume>22</volume><issue>7</issue><spage>933</spage><epage>938</epage><pages>933-938</pages><issn>0043-1354</issn><eissn>1879-2448</eissn><coden>WATRAG</coden><abstract>The inactivation mechanism of tobacco mosaic virus (TMV) in a phosphate buffer (pH 6.9) by ozone was studied. We previously reported that the damage of naked TMV-RNA occurred at the guanine moiety of RNA (Shinriki
et al., Biochim. biophys. Acta
655, 323, 1981). In this paper, we clarified the mode of the inactivation of TMV by using tritium-labeled TMV (TMV
∗) prepared by the reconstitution of tritium-labeled TMV-RNA (TMV-RNA
∗) and coat protein of TMV. It was found that the amount of extracted TMV-RNA
∗ from ozone-treated TMV
∗ decreased with the advance of ozonization, and that there was good correlation between the loss of infectivity and the decrease of recovery of TMV-RNA
∗. When TMV lost its infectivity due to ozone, tryptophan and tyrosine of the coat protein were also degraded by ozone. Polyacrylamide gel electrophoretic analysis of the substance produced during ozonization showed that the coat protein subunits were aggregated with each other and cross-linked with TMV-RNA
∗. From these results, it was concluded that the inability of uncoating is the major cause of the TMV inactivation by ozone.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/0043-1354(88)90031-0</doi><tpages>6</tpages></addata></record> |
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| language | eng |
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| subjects | Action of physical and chemical agents Biological and medical sciences degradation Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control inactivation infectivity Microbiology ozone ozonization Phytopathology. Animal pests. Plant and forest protection Plant viruses and viroids protein coat RNA tobacco mosaic virus tryptophan tyrosine Virology |
| title | Mechanism of inactivation of tobacco mosaic virus with ozone |
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