Translational control during the acute phase response: Ferritin synthesis in response to interleukin-1
Interleukin-1 (IL-1 beta) increases the synthesis of both heavy and light (L)-ferritin subunits when added to human hepatoma cells (HepG2) grown in culture. RNase protection and Northern blot analysis with L-ferritin probes revealed that no changes in L-ferritin mRNA levels occur after cytokine stim...
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| Veröffentlicht in: | The Journal of biological chemistry 1990-08, Vol.265 (24), p.14572-14578 |
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| container_title | The Journal of biological chemistry |
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| creator | ROGERS, J. T BRIDGE, K. R DURMOWICZ, G. P GLASS, J AURON, P. E MUNRO, H. N |
| description | Interleukin-1 (IL-1 beta) increases the synthesis of both heavy and light (L)-ferritin subunits when added to human hepatoma
cells (HepG2) grown in culture. RNase protection and Northern blot analysis with L-ferritin probes revealed that no changes
in L-ferritin mRNA levels occur after cytokine stimulation. However, the induction coincides with an increased association
of the L-subunit mRNA with polyribosomes. Since the recruitment of stored ferritin mRNA onto polyribosomes is seen when iron
enters the cell, the effect of IL-1 beta on iron uptake was tested and was found to be unaffected by the lymphokine. Neither
transferrin receptor mRNA levels nor the number of receptors displayed on the cell surface was affected by IL-1 beta. However,
the action of the cytokine on ferritin translation is inhibited by the action of the intracellular iron chelator deferoxamine.
These data indicate that IL-1 beta induces ferritin gene expression by translational control of its mRNA. The pathway of induction
is different from iron-dependent ferritin gene expression whereas regulation requires the background presence of cellular
iron. |
| doi_str_mv | 10.1016/s0021-9258(18)77341-9 |
| format | Article |
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cells (HepG2) grown in culture. RNase protection and Northern blot analysis with L-ferritin probes revealed that no changes
in L-ferritin mRNA levels occur after cytokine stimulation. However, the induction coincides with an increased association
of the L-subunit mRNA with polyribosomes. Since the recruitment of stored ferritin mRNA onto polyribosomes is seen when iron
enters the cell, the effect of IL-1 beta on iron uptake was tested and was found to be unaffected by the lymphokine. Neither
transferrin receptor mRNA levels nor the number of receptors displayed on the cell surface was affected by IL-1 beta. However,
the action of the cytokine on ferritin translation is inhibited by the action of the intracellular iron chelator deferoxamine.
These data indicate that IL-1 beta induces ferritin gene expression by translational control of its mRNA. The pathway of induction
is different from iron-dependent ferritin gene expression whereas regulation requires the background presence of cellular
iron.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/s0021-9258(18)77341-9</identifier><identifier>PMID: 1696948</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Acute-Phase Proteins - biosynthesis ; Acute-Phase Proteins - genetics ; Analysis of the immune response. Humoral and cellular immunity ; arn mensajero ; arn messager ; Biological and medical sciences ; Biological Transport ; biosintesis ; biosynthese ; biosynthesis ; Carcinoma, Hepatocellular ; Cell Line ; Deferoxamine - pharmacology ; expresion genica ; expression des genes ; facteur immunologique ; factores inmunologicos ; Ferritins - biosynthesis ; Ferritins - genetics ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; gene expression ; Humans ; Immunobiology ; immunological factors ; Interleukin-1 - pharmacology ; Iron - metabolism ; Iron - pharmacology ; Liver - metabolism ; Liver Neoplasms ; Lymphokines, interleukins ( function, expression) ; Macromolecular Substances ; messenger rna ; metalloproteine ; metalloproteins ; metalproteinas ; Nucleic Acid Hybridization ; Polyribosomes - metabolism ; Protein Biosynthesis - drug effects ; Recombinant Proteins - pharmacology ; Regulatory factors and their cellular receptors ; RNA, Messenger - genetics ; RNA, Messenger - isolation & purification ; RNA, Neoplasm - genetics ; RNA, Neoplasm - isolation & purification ; Tumor Cells, Cultured - drug effects ; Tumor Cells, Cultured - metabolism</subject><ispartof>The Journal of biological chemistry, 1990-08, Vol.265 (24), p.14572-14578</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c530t-be02b9c0bf4a7d0e5398d2349b5b5f7262785206192f1ad8b7c8cc83967038823</citedby><cites>FETCH-LOGICAL-c530t-be02b9c0bf4a7d0e5398d2349b5b5f7262785206192f1ad8b7c8cc83967038823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19521518$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1696948$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ROGERS, J. T</creatorcontrib><creatorcontrib>BRIDGE, K. R</creatorcontrib><creatorcontrib>DURMOWICZ, G. P</creatorcontrib><creatorcontrib>GLASS, J</creatorcontrib><creatorcontrib>AURON, P. E</creatorcontrib><creatorcontrib>MUNRO, H. N</creatorcontrib><creatorcontrib>Brigham and Women' s Hospital, Boston, MA</creatorcontrib><title>Translational control during the acute phase response: Ferritin synthesis in response to interleukin-1</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Interleukin-1 (IL-1 beta) increases the synthesis of both heavy and light (L)-ferritin subunits when added to human hepatoma
cells (HepG2) grown in culture. RNase protection and Northern blot analysis with L-ferritin probes revealed that no changes
in L-ferritin mRNA levels occur after cytokine stimulation. However, the induction coincides with an increased association
of the L-subunit mRNA with polyribosomes. Since the recruitment of stored ferritin mRNA onto polyribosomes is seen when iron
enters the cell, the effect of IL-1 beta on iron uptake was tested and was found to be unaffected by the lymphokine. Neither
transferrin receptor mRNA levels nor the number of receptors displayed on the cell surface was affected by IL-1 beta. However,
the action of the cytokine on ferritin translation is inhibited by the action of the intracellular iron chelator deferoxamine.
These data indicate that IL-1 beta induces ferritin gene expression by translational control of its mRNA. The pathway of induction
is different from iron-dependent ferritin gene expression whereas regulation requires the background presence of cellular
iron.</description><subject>Acute-Phase Proteins - biosynthesis</subject><subject>Acute-Phase Proteins - genetics</subject><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>arn mensajero</subject><subject>arn messager</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>biosintesis</subject><subject>biosynthese</subject><subject>biosynthesis</subject><subject>Carcinoma, Hepatocellular</subject><subject>Cell Line</subject><subject>Deferoxamine - pharmacology</subject><subject>expresion genica</subject><subject>expression des genes</subject><subject>facteur immunologique</subject><subject>factores inmunologicos</subject><subject>Ferritins - biosynthesis</subject><subject>Ferritins - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>gene expression</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>immunological factors</subject><subject>Interleukin-1 - pharmacology</subject><subject>Iron - metabolism</subject><subject>Iron - pharmacology</subject><subject>Liver - metabolism</subject><subject>Liver Neoplasms</subject><subject>Lymphokines, interleukins ( function, expression)</subject><subject>Macromolecular Substances</subject><subject>messenger rna</subject><subject>metalloproteine</subject><subject>metalloproteins</subject><subject>metalproteinas</subject><subject>Nucleic Acid Hybridization</subject><subject>Polyribosomes - metabolism</subject><subject>Protein Biosynthesis - drug effects</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Regulatory factors and their cellular receptors</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - isolation & purification</subject><subject>RNA, Neoplasm - genetics</subject><subject>RNA, Neoplasm - isolation & purification</subject><subject>Tumor Cells, Cultured - drug effects</subject><subject>Tumor Cells, Cultured - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkFFv1SAYhonRzLPpT5jhQhe96OSDUsA7s7hpsmQX2xLvCKX0FO0pZ0Bj9u-l9rhxQ968z_cRHoROgZwDgeZzIoRCpSiXH0F-EoLVJb1AGyCSVYzDz5do84S8Rscp_SLl1AqO0BE0qlG13KD-LpopjSb7MJkR2zDlGEbczdFPW5wHh42ds8P7wSSHo0v7MCX3BV-6GH32E06PU6GST7iE_z3OocTs4ujm336q4A161ZsxubeH-wTdX367u_heXd9c_bj4el1ZzkiuWkdoqyxp-9qIjjjOlOwoq1XLW94L2lAhOSUNKNqD6WQrrLRWMtUIwqSk7ASdrXv3MTzMLmW988m6cTSTC3PSwCUIAnUB-QraGFKKrtf76HcmPmogevGrbxd5epGnQep_frUqc6eHB-Z257rnqVVo6T8cepOsGfti1_r0jClOgcPCvV-5wW-HPz463fpgB7fTtOGa1hpqLpb_vFux3gRttrGsur8FpYAQkIop9hfHp5i8</recordid><startdate>19900825</startdate><enddate>19900825</enddate><creator>ROGERS, J. T</creator><creator>BRIDGE, K. R</creator><creator>DURMOWICZ, G. P</creator><creator>GLASS, J</creator><creator>AURON, P. E</creator><creator>MUNRO, H. N</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>H94</scope></search><sort><creationdate>19900825</creationdate><title>Translational control during the acute phase response: Ferritin synthesis in response to interleukin-1</title><author>ROGERS, J. T ; BRIDGE, K. R ; DURMOWICZ, G. P ; GLASS, J ; AURON, P. E ; MUNRO, H. N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c530t-be02b9c0bf4a7d0e5398d2349b5b5f7262785206192f1ad8b7c8cc83967038823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Acute-Phase Proteins - biosynthesis</topic><topic>Acute-Phase Proteins - genetics</topic><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>arn mensajero</topic><topic>arn messager</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>biosintesis</topic><topic>biosynthese</topic><topic>biosynthesis</topic><topic>Carcinoma, Hepatocellular</topic><topic>Cell Line</topic><topic>Deferoxamine - pharmacology</topic><topic>expresion genica</topic><topic>expression des genes</topic><topic>facteur immunologique</topic><topic>factores inmunologicos</topic><topic>Ferritins - biosynthesis</topic><topic>Ferritins - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>gene expression</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>immunological factors</topic><topic>Interleukin-1 - pharmacology</topic><topic>Iron - metabolism</topic><topic>Iron - pharmacology</topic><topic>Liver - metabolism</topic><topic>Liver Neoplasms</topic><topic>Lymphokines, interleukins ( function, expression)</topic><topic>Macromolecular Substances</topic><topic>messenger rna</topic><topic>metalloproteine</topic><topic>metalloproteins</topic><topic>metalproteinas</topic><topic>Nucleic Acid Hybridization</topic><topic>Polyribosomes - metabolism</topic><topic>Protein Biosynthesis - drug effects</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Regulatory factors and their cellular receptors</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - isolation & purification</topic><topic>RNA, Neoplasm - genetics</topic><topic>RNA, Neoplasm - isolation & purification</topic><topic>Tumor Cells, Cultured - drug effects</topic><topic>Tumor Cells, Cultured - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ROGERS, J. T</creatorcontrib><creatorcontrib>BRIDGE, K. R</creatorcontrib><creatorcontrib>DURMOWICZ, G. P</creatorcontrib><creatorcontrib>GLASS, J</creatorcontrib><creatorcontrib>AURON, P. E</creatorcontrib><creatorcontrib>MUNRO, H. N</creatorcontrib><creatorcontrib>Brigham and Women' s Hospital, Boston, MA</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ROGERS, J. T</au><au>BRIDGE, K. R</au><au>DURMOWICZ, G. P</au><au>GLASS, J</au><au>AURON, P. E</au><au>MUNRO, H. N</au><aucorp>Brigham and Women' s Hospital, Boston, MA</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Translational control during the acute phase response: Ferritin synthesis in response to interleukin-1</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1990-08-25</date><risdate>1990</risdate><volume>265</volume><issue>24</issue><spage>14572</spage><epage>14578</epage><pages>14572-14578</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Interleukin-1 (IL-1 beta) increases the synthesis of both heavy and light (L)-ferritin subunits when added to human hepatoma
cells (HepG2) grown in culture. RNase protection and Northern blot analysis with L-ferritin probes revealed that no changes
in L-ferritin mRNA levels occur after cytokine stimulation. However, the induction coincides with an increased association
of the L-subunit mRNA with polyribosomes. Since the recruitment of stored ferritin mRNA onto polyribosomes is seen when iron
enters the cell, the effect of IL-1 beta on iron uptake was tested and was found to be unaffected by the lymphokine. Neither
transferrin receptor mRNA levels nor the number of receptors displayed on the cell surface was affected by IL-1 beta. However,
the action of the cytokine on ferritin translation is inhibited by the action of the intracellular iron chelator deferoxamine.
These data indicate that IL-1 beta induces ferritin gene expression by translational control of its mRNA. The pathway of induction
is different from iron-dependent ferritin gene expression whereas regulation requires the background presence of cellular
iron.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>1696948</pmid><doi>10.1016/s0021-9258(18)77341-9</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1990-08, Vol.265 (24), p.14572-14578 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15817014 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Acute-Phase Proteins - biosynthesis Acute-Phase Proteins - genetics Analysis of the immune response. Humoral and cellular immunity arn mensajero arn messager Biological and medical sciences Biological Transport biosintesis biosynthese biosynthesis Carcinoma, Hepatocellular Cell Line Deferoxamine - pharmacology expresion genica expression des genes facteur immunologique factores inmunologicos Ferritins - biosynthesis Ferritins - genetics Fundamental and applied biological sciences. Psychology Fundamental immunology gene expression Humans Immunobiology immunological factors Interleukin-1 - pharmacology Iron - metabolism Iron - pharmacology Liver - metabolism Liver Neoplasms Lymphokines, interleukins ( function, expression) Macromolecular Substances messenger rna metalloproteine metalloproteins metalproteinas Nucleic Acid Hybridization Polyribosomes - metabolism Protein Biosynthesis - drug effects Recombinant Proteins - pharmacology Regulatory factors and their cellular receptors RNA, Messenger - genetics RNA, Messenger - isolation & purification RNA, Neoplasm - genetics RNA, Neoplasm - isolation & purification Tumor Cells, Cultured - drug effects Tumor Cells, Cultured - metabolism |
| title | Translational control during the acute phase response: Ferritin synthesis in response to interleukin-1 |
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