Characterization of a primary hepatocyte culture system for toxicological studies

An hepatocyte culture system was developed for potential use in toxicological studies in vitro. Rat hepatocytes were isolated by two-step collagenase perfusion and cultured on Vitrogen-coated Permanox™ dishes in a modified Chee's medium containing 1 μM dexamethasone and 1% dimethylsulfoxide. Th...

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Veröffentlicht in:	In vitro cellular & developmental biology. Animal 1996-04, Vol.32 (4), p.211-220
Hauptverfasser:	Zurlo, J. (Johns Hopkins Center for Alternatives to Animal Testing, Baltimore, MD.), Arterburn, L.M
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ALBUMINAS ALBUMINE ALBUMINS Albumins - metabolism ALTERNATIVAS A ENSAYOS CON ANIMALES ALTERNATIVE ANIMAL DE LABORATOIRE ANIMAL TESTING ALTERNATIVES Animals CELL CULTURE Cell Culture Techniques Cell Survival CELLS Cellular and Molecular Toxicology Cellular metabolism CELLULE CELULAS CITOCROMO P 450 CITOCROMOS COMPOSE ORGANOSOUFRE COMPUESTO ORGANICO DEL AZUFRE CULTIVO DE CELULAS CULTURE DE CELLULE CULTURE MEDIA Cultured cells CYTOCHROME CYTOCHROME P 450 Cytochrome P-450 CYP1A1 - metabolism Cytochrome P-450 CYP2B1 - metabolism Cytochrome P-450 CYP3A Cytochrome P-450 Enzyme System - metabolism CYTOCHROMES DEXAMETASONA DEXAMETHASONE Dexamethasone - pharmacology Diazepam - metabolism DIMETHYL SULFOXIDE Dimethyl Sulfoxide - pharmacology Enzymes ENZYMIC ACTIVITY FOIE GLICOSILTRANSFERASAS Glucuronosyltransferase - metabolism GLUTATHION GLUTATHIONE Glutathione - metabolism GLUTATHIONE TRANSFERASE Glutathione Transferase - metabolism GLUTATION GLYCOSYLTRANSFERASE GLYCOSYLTRANSFERASES Hepatocytes HEXOSYLTRANSFERASES HIGADO LIVER Liver - cytology Liver - metabolism Male MEDIO DE CULTIVO Messenger RNA MILIEU DE CULTURE Mixed Function Oxygenases - metabolism ORGANOSULPHUR COMPOUNDS OXIDOREDUCTASES OXIDORREDUCTASAS OXYDOREDUCTASE OXYGENASES Plastics PROTEINAS PROTEINE PROTEINS RAT RATA RATS Rats, Inbred F344 SECRECION SECRETION Testosterone Testosterone - metabolism Toxicity Tests TOXICOLOGIA TOXICOLOGIE TOXICOLOGY TRANSFERASAS TRANSFERASE TRANSFERASES udp-glucuronosyltransferase
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