Cloning and expression of AatII restriction-modification system in Escherichia coli

The genes encoding the AatII restriction endonuclease and methylase from Acetobacter aceti have been cloned and expressed in Escherichia coli. The nucleotide sequences of aatIIM and aatIIR genes were determined. The aatIIM and aatIIR genes are 996 bp and 1038 bp, respectively, encoding the 331-aa me...

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Veröffentlicht in:	Gene 1997, Vol.185 (1), p.105-109
Hauptverfasser:	Nwankwo, Donald O, Maunus, Robert E, Xu, Shuang-yong
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetobacter - enzymology Acetobacter - genetics Acetobacter aceti Amino Acid Sequence Bacteriophage T7 - genetics Base Sequence Chromatography Cloning, Molecular Deoxyribonucleases, Type II Site-Specific - genetics Deoxyribonucleases, Type II Site-Specific - metabolism DNA Modification Methylases - genetics DNA Modification Methylases - metabolism Electrophoresis, Polyacrylamide Gel Endonuclease Escherichia coli Escherichia coli - genetics Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Methylase Molecular Sequence Data Mutagenesis, Insertional Plasmids - genetics Promoter Regions, Genetic Protein purification Recombinant Proteins - isolation & purification Restriction Mapping Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid T7 expression vector Transcription, Genetic
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