Plasmids for ectopic integration in Bacillus subtilis

Plasmids have been constructed that allow integration by a double recombination event at the thrC locus of the Bacillus subtilis (Bs) chromosome. These plasmids can be used either for construction of merodiploid strains and complementation analysis, or for construction of transcriptional fusions to...

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Bibliographische Detailangaben
Veröffentlicht in:	Gene 1996-11, Vol.180 (1), p.57-61
Hauptverfasser:	Guérout-Fleury, Anne-Marie, Frandsen, Niels, Stragier, Patrick
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha-Amylases - genetics amyE locus Bacillus subtilis Bacillus subtilis - genetics Carbon-Oxygen Lyases Double recombination event Drug Resistance, Microbial - genetics Genetic Vectors lacZ fusion Lyases - genetics Merodiploid Molecular Sequence Data Plasmids Recombination, Genetic thrC locus Transduction, Genetic Transformation, Genetic
Online-Zugang:	Volltext
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