Plasmids for ectopic integration in Bacillus subtilis
Plasmids have been constructed that allow integration by a double recombination event at the thrC locus of the Bacillus subtilis (Bs) chromosome. These plasmids can be used either for construction of merodiploid strains and complementation analysis, or for construction of transcriptional fusions to...
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| Veröffentlicht in: | Gene 1996-11, Vol.180 (1), p.57-61 |
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| creator | Guérout-Fleury, Anne-Marie Frandsen, Niels Stragier, Patrick |
| description | Plasmids have been constructed that allow integration by a double recombination event at the
thrC locus of the
Bacillus subtilis (Bs) chromosome. These plasmids can be used either for construction of merodiploid strains and complementation analysis, or for construction of transcriptional fusions to the
Escherichia coli lacZ gene. The plasmids contain an antibiotic (An) marker selectable in
Bs, as well as an additional An marker outside of the region that can recombine into the chromosome. When used in conjunction with recipient strains containing a third An marker at their
thrC locus, these plasmids allow easy identification of transformants issued from a marker exchange event without additional Campbell-type integration. The existing plasmids used for ectopic integration at the
amyE locus have been modified similarly. |
| doi_str_mv | 10.1016/S0378-1119(96)00404-0 |
| format | Article |
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thrC locus of the
Bacillus subtilis (Bs) chromosome. These plasmids can be used either for construction of merodiploid strains and complementation analysis, or for construction of transcriptional fusions to the
Escherichia coli lacZ gene. The plasmids contain an antibiotic (An) marker selectable in
Bs, as well as an additional An marker outside of the region that can recombine into the chromosome. When used in conjunction with recipient strains containing a third An marker at their
thrC locus, these plasmids allow easy identification of transformants issued from a marker exchange event without additional Campbell-type integration. The existing plasmids used for ectopic integration at the
amyE locus have been modified similarly.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/S0378-1119(96)00404-0</identifier><identifier>PMID: 8973347</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>alpha-Amylases - genetics ; amyE locus ; Bacillus subtilis ; Bacillus subtilis - genetics ; Carbon-Oxygen Lyases ; Double recombination event ; Drug Resistance, Microbial - genetics ; Genetic Vectors ; lacZ fusion ; Lyases - genetics ; Merodiploid ; Molecular Sequence Data ; Plasmids ; Recombination, Genetic ; thrC locus ; Transduction, Genetic ; Transformation, Genetic</subject><ispartof>Gene, 1996-11, Vol.180 (1), p.57-61</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-cdae6382f871fbd19f230e01c0705bec7cc3a6617586f734402f150a7d7ad24f3</citedby><cites>FETCH-LOGICAL-c478t-cdae6382f871fbd19f230e01c0705bec7cc3a6617586f734402f150a7d7ad24f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-1119(96)00404-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8973347$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guérout-Fleury, Anne-Marie</creatorcontrib><creatorcontrib>Frandsen, Niels</creatorcontrib><creatorcontrib>Stragier, Patrick</creatorcontrib><title>Plasmids for ectopic integration in Bacillus subtilis</title><title>Gene</title><addtitle>Gene</addtitle><description>Plasmids have been constructed that allow integration by a double recombination event at the
thrC locus of the
Bacillus subtilis (Bs) chromosome. These plasmids can be used either for construction of merodiploid strains and complementation analysis, or for construction of transcriptional fusions to the
Escherichia coli lacZ gene. The plasmids contain an antibiotic (An) marker selectable in
Bs, as well as an additional An marker outside of the region that can recombine into the chromosome. When used in conjunction with recipient strains containing a third An marker at their
thrC locus, these plasmids allow easy identification of transformants issued from a marker exchange event without additional Campbell-type integration. The existing plasmids used for ectopic integration at the
amyE locus have been modified similarly.</description><subject>alpha-Amylases - genetics</subject><subject>amyE locus</subject><subject>Bacillus subtilis</subject><subject>Bacillus subtilis - genetics</subject><subject>Carbon-Oxygen Lyases</subject><subject>Double recombination event</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Genetic Vectors</subject><subject>lacZ fusion</subject><subject>Lyases - genetics</subject><subject>Merodiploid</subject><subject>Molecular Sequence Data</subject><subject>Plasmids</subject><subject>Recombination, Genetic</subject><subject>thrC locus</subject><subject>Transduction, Genetic</subject><subject>Transformation, Genetic</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkFtLxDAQhYMo67r6Exb6JPpQnWmaJn0SXbzBgoL6HNJcJNLLmrSC_97uhX11XmbgnJnDfITMEa4QsLh-A8pFiojlRVlcAuSQp3BApih4mQJQcUime8sxOYnxC8ZiLJuQiSg5pTmfEvZaq9h4ExPXhcTqvlt5nfi2t59B9b5rxzm5U9rX9RCTOFS9r308JUdO1dGe7fqMfDzcvy-e0uXL4_PidpnqnIs-1UbZgorMCY6uMli6jIIF1MCBVVZzrakqCuRMFI7TPIfMIQPFDVcmyx2dkfPt3VXovgcbe9n4qG1dq9Z2Q5TIeJmBwNHItkYduhiDdXIVfKPCr0SQa1xyg0uuWciykBtcEsa9-S5gqBpr9ls7PqN-s9Xt-OWPt0FG7W2rrfFhhCVN5_9J-AMnk3kh</recordid><startdate>19961121</startdate><enddate>19961121</enddate><creator>Guérout-Fleury, Anne-Marie</creator><creator>Frandsen, Niels</creator><creator>Stragier, Patrick</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope></search><sort><creationdate>19961121</creationdate><title>Plasmids for ectopic integration in Bacillus subtilis</title><author>Guérout-Fleury, Anne-Marie ; Frandsen, Niels ; Stragier, Patrick</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-cdae6382f871fbd19f230e01c0705bec7cc3a6617586f734402f150a7d7ad24f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>alpha-Amylases - genetics</topic><topic>amyE locus</topic><topic>Bacillus subtilis</topic><topic>Bacillus subtilis - genetics</topic><topic>Carbon-Oxygen Lyases</topic><topic>Double recombination event</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Genetic Vectors</topic><topic>lacZ fusion</topic><topic>Lyases - genetics</topic><topic>Merodiploid</topic><topic>Molecular Sequence Data</topic><topic>Plasmids</topic><topic>Recombination, Genetic</topic><topic>thrC locus</topic><topic>Transduction, Genetic</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guérout-Fleury, Anne-Marie</creatorcontrib><creatorcontrib>Frandsen, Niels</creatorcontrib><creatorcontrib>Stragier, Patrick</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guérout-Fleury, Anne-Marie</au><au>Frandsen, Niels</au><au>Stragier, Patrick</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasmids for ectopic integration in Bacillus subtilis</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1996-11-21</date><risdate>1996</risdate><volume>180</volume><issue>1</issue><spage>57</spage><epage>61</epage><pages>57-61</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>Plasmids have been constructed that allow integration by a double recombination event at the
thrC locus of the
Bacillus subtilis (Bs) chromosome. These plasmids can be used either for construction of merodiploid strains and complementation analysis, or for construction of transcriptional fusions to the
Escherichia coli lacZ gene. The plasmids contain an antibiotic (An) marker selectable in
Bs, as well as an additional An marker outside of the region that can recombine into the chromosome. When used in conjunction with recipient strains containing a third An marker at their
thrC locus, these plasmids allow easy identification of transformants issued from a marker exchange event without additional Campbell-type integration. The existing plasmids used for ectopic integration at the
amyE locus have been modified similarly.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8973347</pmid><doi>10.1016/S0378-1119(96)00404-0</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1996-11, Vol.180 (1), p.57-61 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15792081 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | alpha-Amylases - genetics amyE locus Bacillus subtilis Bacillus subtilis - genetics Carbon-Oxygen Lyases Double recombination event Drug Resistance, Microbial - genetics Genetic Vectors lacZ fusion Lyases - genetics Merodiploid Molecular Sequence Data Plasmids Recombination, Genetic thrC locus Transduction, Genetic Transformation, Genetic |
| title | Plasmids for ectopic integration in Bacillus subtilis |
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