Biomonitoring of diesel exhaust-exposed workers. DNA and hemoglobin adducts and urinary 1-hydroxypyrene as markers of exposure
Diesel exhaust-exposed workers have been shown to have an increased risk of lung cancer. A battery of biomarkers were evaluated for their ability to assess differences in exposure to genotoxic compounds in bus garage workers and mechanics and controls. Lymphocyte DNA adducts were analyzed using the...
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| Veröffentlicht in: | Toxicology letters 1996-07, Vol.86 (1), p.27-37 |
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| creator | Nielsen, Per Sabro Andreassen, Åshild Farmer, Peter B. Ørebø, Steinar Autrup, Herman |
| description | Diesel exhaust-exposed workers have been shown to have an increased risk of lung cancer. A battery of biomarkers were evaluated for their ability to assess differences in exposure to genotoxic compounds in bus garage workers and mechanics and controls. Lymphocyte DNA adducts were analyzed using the
32P-postlabelling method with butanol and P1 enrichment procedures. Hydroxyethylvaline (HOEtVal) adducts in hemoglobin were measured by gas chromatography-mass spectrometry (GC-MS) and 1-hydroxypyrene (HPU) in urine determined using HPLC analysis. The exposed workers had significantly higher levels of all three biomarkers compared to the controls. Total DNA adduct levels were 0.84 fmol/μg DNA vs. 0.26 in controls (butanol) and 0.65 fmol/μg DNA vs. 0.08 (P1 nuclease). Median HOEtVal adduct level in exposed workers was 33.3 pmol/g hemoglobin vs. 22.1 in controls. HOEtVal adducts correlated with HPU but not with DNA adducts. The levels of HPU in urine were 0.11 μmol/mol creatinine compared to 0.05 in controls. All three assays applied were sensitive enough to evaluate a low level of exposure to environmental pollutants, with posllabelling and GC-MS as the most sensitive assays. The study indicated that skin absorption of polycyclic aromatic hydrocarbons (PAH) might be an important factor to consider when studying PAH exposure from air pollution sources. |
| doi_str_mv | 10.1016/0378-4274(96)83963-4 |
| format | Article |
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32P-postlabelling method with butanol and P1 enrichment procedures. Hydroxyethylvaline (HOEtVal) adducts in hemoglobin were measured by gas chromatography-mass spectrometry (GC-MS) and 1-hydroxypyrene (HPU) in urine determined using HPLC analysis. The exposed workers had significantly higher levels of all three biomarkers compared to the controls. Total DNA adduct levels were 0.84 fmol/μg DNA vs. 0.26 in controls (butanol) and 0.65 fmol/μg DNA vs. 0.08 (P1 nuclease). Median HOEtVal adduct level in exposed workers was 33.3 pmol/g hemoglobin vs. 22.1 in controls. HOEtVal adducts correlated with HPU but not with DNA adducts. The levels of HPU in urine were 0.11 μmol/mol creatinine compared to 0.05 in controls. All three assays applied were sensitive enough to evaluate a low level of exposure to environmental pollutants, with posllabelling and GC-MS as the most sensitive assays. The study indicated that skin absorption of polycyclic aromatic hydrocarbons (PAH) might be an important factor to consider when studying PAH exposure from air pollution sources.</description><subject>1-Hydroxypyrene</subject><subject>32P-Postlabelling</subject><subject>Adult</subject><subject>Air</subject><subject>Air Pollutants, Occupational - adverse effects</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>Biomarkers - urine</subject><subject>Biomonitoring</subject><subject>Cross-Sectional Studies</subject><subject>DNA adducts</subject><subject>DNA Adducts - blood</subject><subject>Environmental Monitoring - methods</subject><subject>Environmental pollutants toxicology</subject><subject>Hemoglobins - chemistry</subject><subject>HOEtVal hemoglobin adducts</subject><subject>Humans</subject><subject>Lymphocytes - chemistry</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Mutagens - analysis</subject><subject>Occupational Exposure - adverse effects</subject><subject>PAH</subject><subject>Phosphorus Radioisotopes</subject><subject>Pyrenes - analysis</subject><subject>Toxicology</subject><subject>Vehicle Emissions - adverse effects</subject><issn>0378-4274</issn><issn>1879-3169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMuO0zAUQC0EGsrAH4DkBUKwyOBbp35skIbhKY1gA2vLsa-nhiQudgLNhm8naasuWVmyzz2-OoQ8BXYFDMRrxqWq6rWsX2rxSnEteFXfIytQUlcchL5PVmfkIXlUyg_GmKjF5oJcKKE2GuSK_H0bU5f6OKQc-zuaAvURC7YU91s7lqHC_S4V9PRPyj8xlyv67ss1tb2nW-zSXZua2FPr_eiGcrgeZ4_NE4VqO_mc9tNuytgjtYV29qBYPjlYx4yPyYNg24JPTucl-f7h_bebT9Xt14-fb65vK1eDHKq10BKaRinwqEITrIYQvFRaOq4aKTRHxx0CyEbqTQ1MOCFscJrJoBQKfkleHL27nH6NWAbTxeKwbW2PaSwGNlIJxuoZrI-gy6mUjMHscpwXnwwws2Q3S1OzNDVamEN2s4w9O_nHpkN_Hjp1nt-fn95tcbYN2fYuljPGYb2evTP25ojh3OJ3xGyKi9g79DGjG4xP8f97_ANeuaCr</recordid><startdate>19960701</startdate><enddate>19960701</enddate><creator>Nielsen, Per Sabro</creator><creator>Andreassen, Åshild</creator><creator>Farmer, Peter B.</creator><creator>Ørebø, Steinar</creator><creator>Autrup, Herman</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T2</scope><scope>7U2</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19960701</creationdate><title>Biomonitoring of diesel exhaust-exposed workers. DNA and hemoglobin adducts and urinary 1-hydroxypyrene as markers of exposure</title><author>Nielsen, Per Sabro ; Andreassen, Åshild ; Farmer, Peter B. ; Ørebø, Steinar ; Autrup, Herman</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-26971bb881de8fbfa91ffd7897c38b7693ec3ce117b7954106c66afc907f88e63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>1-Hydroxypyrene</topic><topic>32P-Postlabelling</topic><topic>Adult</topic><topic>Air</topic><topic>Air Pollutants, Occupational - adverse effects</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>Biomarkers - urine</topic><topic>Biomonitoring</topic><topic>Cross-Sectional Studies</topic><topic>DNA adducts</topic><topic>DNA Adducts - blood</topic><topic>Environmental Monitoring - methods</topic><topic>Environmental pollutants toxicology</topic><topic>Hemoglobins - chemistry</topic><topic>HOEtVal hemoglobin adducts</topic><topic>Humans</topic><topic>Lymphocytes - chemistry</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Mutagens - analysis</topic><topic>Occupational Exposure - adverse effects</topic><topic>PAH</topic><topic>Phosphorus Radioisotopes</topic><topic>Pyrenes - analysis</topic><topic>Toxicology</topic><topic>Vehicle Emissions - adverse effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nielsen, Per Sabro</creatorcontrib><creatorcontrib>Andreassen, Åshild</creatorcontrib><creatorcontrib>Farmer, Peter B.</creatorcontrib><creatorcontrib>Ørebø, Steinar</creatorcontrib><creatorcontrib>Autrup, Herman</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Toxicology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nielsen, Per Sabro</au><au>Andreassen, Åshild</au><au>Farmer, Peter B.</au><au>Ørebø, Steinar</au><au>Autrup, Herman</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biomonitoring of diesel exhaust-exposed workers. DNA and hemoglobin adducts and urinary 1-hydroxypyrene as markers of exposure</atitle><jtitle>Toxicology letters</jtitle><addtitle>Toxicol Lett</addtitle><date>1996-07-01</date><risdate>1996</risdate><volume>86</volume><issue>1</issue><spage>27</spage><epage>37</epage><pages>27-37</pages><issn>0378-4274</issn><eissn>1879-3169</eissn><coden>TOLED5</coden><abstract>Diesel exhaust-exposed workers have been shown to have an increased risk of lung cancer. A battery of biomarkers were evaluated for their ability to assess differences in exposure to genotoxic compounds in bus garage workers and mechanics and controls. Lymphocyte DNA adducts were analyzed using the
32P-postlabelling method with butanol and P1 enrichment procedures. Hydroxyethylvaline (HOEtVal) adducts in hemoglobin were measured by gas chromatography-mass spectrometry (GC-MS) and 1-hydroxypyrene (HPU) in urine determined using HPLC analysis. The exposed workers had significantly higher levels of all three biomarkers compared to the controls. Total DNA adduct levels were 0.84 fmol/μg DNA vs. 0.26 in controls (butanol) and 0.65 fmol/μg DNA vs. 0.08 (P1 nuclease). Median HOEtVal adduct level in exposed workers was 33.3 pmol/g hemoglobin vs. 22.1 in controls. HOEtVal adducts correlated with HPU but not with DNA adducts. The levels of HPU in urine were 0.11 μmol/mol creatinine compared to 0.05 in controls. All three assays applied were sensitive enough to evaluate a low level of exposure to environmental pollutants, with posllabelling and GC-MS as the most sensitive assays. The study indicated that skin absorption of polycyclic aromatic hydrocarbons (PAH) might be an important factor to consider when studying PAH exposure from air pollution sources.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>8685917</pmid><doi>10.1016/0378-4274(96)83963-4</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 0378-4274 |
| ispartof | Toxicology letters, 1996-07, Vol.86 (1), p.27-37 |
| issn | 0378-4274 1879-3169 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 1-Hydroxypyrene 32P-Postlabelling Adult Air Air Pollutants, Occupational - adverse effects Autoradiography Biological and medical sciences Biological Assay Biomarkers - urine Biomonitoring Cross-Sectional Studies DNA adducts DNA Adducts - blood Environmental Monitoring - methods Environmental pollutants toxicology Hemoglobins - chemistry HOEtVal hemoglobin adducts Humans Lymphocytes - chemistry Male Medical sciences Middle Aged Mutagens - analysis Occupational Exposure - adverse effects PAH Phosphorus Radioisotopes Pyrenes - analysis Toxicology Vehicle Emissions - adverse effects |
| title | Biomonitoring of diesel exhaust-exposed workers. DNA and hemoglobin adducts and urinary 1-hydroxypyrene as markers of exposure |
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