Immuno-detection of proteins by cytochrome P450 antibodies in five species of sea anemones
The presence and nature of immunoreactive proteins to several cytochrome P450 (CYP) antibodies in the columnar or whole body microsomal fraction of five sea anemone species ( Anthopleura elegantissima, Aiptasia pallida, Anthopleura xanthogrammica, Bunodosoma cavernata and Condylactis gigantea) were...
Gespeichert in:
| Veröffentlicht in: | Marine environmental research 1996, Vol.42 (1), p.353-357 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 357 |
|---|---|
| container_issue | 1 |
| container_start_page | 353 |
| container_title | Marine environmental research |
| container_volume | 42 |
| creator | Heffernan, Linda M. Ertl, Robin P. Stegeman, John J. Buhler, Donald R. Winston, Gary W. |
| description | The presence and nature of immunoreactive proteins to several cytochrome P450 (CYP) antibodies in the columnar or whole body microsomal fraction of five sea anemone species (
Anthopleura elegantissima, Aiptasia pallida, Anthopleura xanthogrammica, Bunodosoma cavernata and
Condylactis gigantea) were probed by Western blot analysis.
Anthopleura elegantissima, A. xanthogrammica, B. cavernata and
C. gigantea columnar microsomes contained proteins that crossreacted with monoclonal anti-scup CYP 1A1 (~ 70 kDa), polyclonal anti-trout CYP 2K1 (~ 40 kDa) and polyclonal anti-trout CYP 3A1 (~ 30 kDa).
Aiptasia pallida whole body microsomes contained various proteins that cross-reacted with scup CYP 1A1, trout CYP 2K1 and trout CYP 3A1 antibodies. 3-Methylcholantrene (3MC, 20 mg/kg) did not induce proteins recognized by anti-CYP 1A1 in
A. elegantissima. Our results indicate the presence in anemone microsomes of various proteins with epitope regions recognized by several CYP antibodies; the most strongly recognized proteins differ from the 50–60 kDa molecular weights which are typically ascribed to P450s of vertebrate microsomes. In all anemones, proteins in the 50–60 kDa range are very weakly recognized even at relatively high protein concentrations. |
| doi_str_mv | 10.1016/0141-1136(95)00038-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_15785505</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0141113695000380</els_id><sourcerecordid>15741821</sourcerecordid><originalsourceid>FETCH-LOGICAL-c397t-7a16b849a6e6a0130d2def816558af7b8af0f8370db755ad3ea87fb2414908253</originalsourceid><addsrcrecordid>eNqNkU9LxDAQxYMouK5-Aw85iR6qM03TpBdBFv8sLOhBL15C2k4xsm3Wpruw397UFY_qZYaB33sM7zF2inCJgPkVYIYJosjPC3kBAEInsMcmqFWRQFrgPpv8IIfsKIT3CEmFcsJe52277nxS00DV4HzHfcNXvR_IdYGXW15tB1-99b4l_pRJ4LYbXOlrR4G7jjduQzysqBrvqAxkI0Gt7ygcs4PGLgOdfO8pe7m7fZ49JIvH-_nsZpFUolBDoizmpc4Km1NuAQXUaU2NxlxKbRtVxgGNFgrqUklpa0FWq6ZMM8wK0KkUU3a2841vf6wpDKZ1oaLlMj7i18GgVFpK-BeYoU7xb1DkQiIWEcx2YNX7EHpqzKp3re23BsGM1ZgxdzPmbgppvqoxEGXXOxnFWDaOehNigF1FtetjC6b27neDTxktlEo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>13635119</pqid></control><display><type>article</type><title>Immuno-detection of proteins by cytochrome P450 antibodies in five species of sea anemones</title><source>ScienceDirect Journals (5 years ago - present)</source><creator>Heffernan, Linda M. ; Ertl, Robin P. ; Stegeman, John J. ; Buhler, Donald R. ; Winston, Gary W.</creator><contributor>Hinton, DE (eds) ; Hinton, DE</contributor><creatorcontrib>Heffernan, Linda M. ; Ertl, Robin P. ; Stegeman, John J. ; Buhler, Donald R. ; Winston, Gary W. ; Hinton, DE (eds) ; Hinton, DE</creatorcontrib><description>The presence and nature of immunoreactive proteins to several cytochrome P450 (CYP) antibodies in the columnar or whole body microsomal fraction of five sea anemone species (
Anthopleura elegantissima, Aiptasia pallida, Anthopleura xanthogrammica, Bunodosoma cavernata and
Condylactis gigantea) were probed by Western blot analysis.
Anthopleura elegantissima, A. xanthogrammica, B. cavernata and
C. gigantea columnar microsomes contained proteins that crossreacted with monoclonal anti-scup CYP 1A1 (~ 70 kDa), polyclonal anti-trout CYP 2K1 (~ 40 kDa) and polyclonal anti-trout CYP 3A1 (~ 30 kDa).
Aiptasia pallida whole body microsomes contained various proteins that cross-reacted with scup CYP 1A1, trout CYP 2K1 and trout CYP 3A1 antibodies. 3-Methylcholantrene (3MC, 20 mg/kg) did not induce proteins recognized by anti-CYP 1A1 in
A. elegantissima. Our results indicate the presence in anemone microsomes of various proteins with epitope regions recognized by several CYP antibodies; the most strongly recognized proteins differ from the 50–60 kDa molecular weights which are typically ascribed to P450s of vertebrate microsomes. In all anemones, proteins in the 50–60 kDa range are very weakly recognized even at relatively high protein concentrations.</description><identifier>ISSN: 0141-1136</identifier><identifier>EISSN: 1879-0291</identifier><identifier>DOI: 10.1016/0141-1136(95)00038-0</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Actiniaria ; Aiptasia pallida ; Anthopleura ; Anthopleura elegantissima ; Anthopleura xanthogrammica ; Bunodosoma cavernata ; Condylactis gigantea ; Marine</subject><ispartof>Marine environmental research, 1996, Vol.42 (1), p.353-357</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-7a16b849a6e6a0130d2def816558af7b8af0f8370db755ad3ea87fb2414908253</citedby><cites>FETCH-LOGICAL-c397t-7a16b849a6e6a0130d2def816558af7b8af0f8370db755ad3ea87fb2414908253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0141-1136(95)00038-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,4024,4050,4051,23930,23931,25140,27923,27924,27925,45995</link.rule.ids></links><search><contributor>Hinton, DE (eds)</contributor><contributor>Hinton, DE</contributor><creatorcontrib>Heffernan, Linda M.</creatorcontrib><creatorcontrib>Ertl, Robin P.</creatorcontrib><creatorcontrib>Stegeman, John J.</creatorcontrib><creatorcontrib>Buhler, Donald R.</creatorcontrib><creatorcontrib>Winston, Gary W.</creatorcontrib><title>Immuno-detection of proteins by cytochrome P450 antibodies in five species of sea anemones</title><title>Marine environmental research</title><description>The presence and nature of immunoreactive proteins to several cytochrome P450 (CYP) antibodies in the columnar or whole body microsomal fraction of five sea anemone species (
Anthopleura elegantissima, Aiptasia pallida, Anthopleura xanthogrammica, Bunodosoma cavernata and
Condylactis gigantea) were probed by Western blot analysis.
Anthopleura elegantissima, A. xanthogrammica, B. cavernata and
C. gigantea columnar microsomes contained proteins that crossreacted with monoclonal anti-scup CYP 1A1 (~ 70 kDa), polyclonal anti-trout CYP 2K1 (~ 40 kDa) and polyclonal anti-trout CYP 3A1 (~ 30 kDa).
Aiptasia pallida whole body microsomes contained various proteins that cross-reacted with scup CYP 1A1, trout CYP 2K1 and trout CYP 3A1 antibodies. 3-Methylcholantrene (3MC, 20 mg/kg) did not induce proteins recognized by anti-CYP 1A1 in
A. elegantissima. Our results indicate the presence in anemone microsomes of various proteins with epitope regions recognized by several CYP antibodies; the most strongly recognized proteins differ from the 50–60 kDa molecular weights which are typically ascribed to P450s of vertebrate microsomes. In all anemones, proteins in the 50–60 kDa range are very weakly recognized even at relatively high protein concentrations.</description><subject>Actiniaria</subject><subject>Aiptasia pallida</subject><subject>Anthopleura</subject><subject>Anthopleura elegantissima</subject><subject>Anthopleura xanthogrammica</subject><subject>Bunodosoma cavernata</subject><subject>Condylactis gigantea</subject><subject>Marine</subject><issn>0141-1136</issn><issn>1879-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqNkU9LxDAQxYMouK5-Aw85iR6qM03TpBdBFv8sLOhBL15C2k4xsm3Wpruw397UFY_qZYaB33sM7zF2inCJgPkVYIYJosjPC3kBAEInsMcmqFWRQFrgPpv8IIfsKIT3CEmFcsJe52277nxS00DV4HzHfcNXvR_IdYGXW15tB1-99b4l_pRJ4LYbXOlrR4G7jjduQzysqBrvqAxkI0Gt7ygcs4PGLgOdfO8pe7m7fZ49JIvH-_nsZpFUolBDoizmpc4Km1NuAQXUaU2NxlxKbRtVxgGNFgrqUklpa0FWq6ZMM8wK0KkUU3a2841vf6wpDKZ1oaLlMj7i18GgVFpK-BeYoU7xb1DkQiIWEcx2YNX7EHpqzKp3re23BsGM1ZgxdzPmbgppvqoxEGXXOxnFWDaOehNigF1FtetjC6b27neDTxktlEo</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Heffernan, Linda M.</creator><creator>Ertl, Robin P.</creator><creator>Stegeman, John J.</creator><creator>Buhler, Donald R.</creator><creator>Winston, Gary W.</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7TN</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>7U7</scope><scope>7UA</scope><scope>8FD</scope><scope>FR3</scope><scope>H99</scope><scope>L.F</scope><scope>P64</scope></search><sort><creationdate>1996</creationdate><title>Immuno-detection of proteins by cytochrome P450 antibodies in five species of sea anemones</title><author>Heffernan, Linda M. ; Ertl, Robin P. ; Stegeman, John J. ; Buhler, Donald R. ; Winston, Gary W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-7a16b849a6e6a0130d2def816558af7b8af0f8370db755ad3ea87fb2414908253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Actiniaria</topic><topic>Aiptasia pallida</topic><topic>Anthopleura</topic><topic>Anthopleura elegantissima</topic><topic>Anthopleura xanthogrammica</topic><topic>Bunodosoma cavernata</topic><topic>Condylactis gigantea</topic><topic>Marine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heffernan, Linda M.</creatorcontrib><creatorcontrib>Ertl, Robin P.</creatorcontrib><creatorcontrib>Stegeman, John J.</creatorcontrib><creatorcontrib>Buhler, Donald R.</creatorcontrib><creatorcontrib>Winston, Gary W.</creatorcontrib><collection>CrossRef</collection><collection>Aqualine</collection><collection>Oceanic Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Marine environmental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heffernan, Linda M.</au><au>Ertl, Robin P.</au><au>Stegeman, John J.</au><au>Buhler, Donald R.</au><au>Winston, Gary W.</au><au>Hinton, DE (eds)</au><au>Hinton, DE</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immuno-detection of proteins by cytochrome P450 antibodies in five species of sea anemones</atitle><jtitle>Marine environmental research</jtitle><date>1996</date><risdate>1996</risdate><volume>42</volume><issue>1</issue><spage>353</spage><epage>357</epage><pages>353-357</pages><issn>0141-1136</issn><eissn>1879-0291</eissn><abstract>The presence and nature of immunoreactive proteins to several cytochrome P450 (CYP) antibodies in the columnar or whole body microsomal fraction of five sea anemone species (
Anthopleura elegantissima, Aiptasia pallida, Anthopleura xanthogrammica, Bunodosoma cavernata and
Condylactis gigantea) were probed by Western blot analysis.
Anthopleura elegantissima, A. xanthogrammica, B. cavernata and
C. gigantea columnar microsomes contained proteins that crossreacted with monoclonal anti-scup CYP 1A1 (~ 70 kDa), polyclonal anti-trout CYP 2K1 (~ 40 kDa) and polyclonal anti-trout CYP 3A1 (~ 30 kDa).
Aiptasia pallida whole body microsomes contained various proteins that cross-reacted with scup CYP 1A1, trout CYP 2K1 and trout CYP 3A1 antibodies. 3-Methylcholantrene (3MC, 20 mg/kg) did not induce proteins recognized by anti-CYP 1A1 in
A. elegantissima. Our results indicate the presence in anemone microsomes of various proteins with epitope regions recognized by several CYP antibodies; the most strongly recognized proteins differ from the 50–60 kDa molecular weights which are typically ascribed to P450s of vertebrate microsomes. In all anemones, proteins in the 50–60 kDa range are very weakly recognized even at relatively high protein concentrations.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/0141-1136(95)00038-0</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0141-1136 |
| ispartof | Marine environmental research, 1996, Vol.42 (1), p.353-357 |
| issn | 0141-1136 1879-0291 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15785505 |
| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Actiniaria Aiptasia pallida Anthopleura Anthopleura elegantissima Anthopleura xanthogrammica Bunodosoma cavernata Condylactis gigantea Marine |
| title | Immuno-detection of proteins by cytochrome P450 antibodies in five species of sea anemones |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T20%3A24%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immuno-detection%20of%20proteins%20by%20cytochrome%20P450%20antibodies%20in%20five%20species%20of%20sea%20anemones&rft.jtitle=Marine%20environmental%20research&rft.au=Heffernan,%20Linda%20M.&rft.date=1996&rft.volume=42&rft.issue=1&rft.spage=353&rft.epage=357&rft.pages=353-357&rft.issn=0141-1136&rft.eissn=1879-0291&rft_id=info:doi/10.1016/0141-1136(95)00038-0&rft_dat=%3Cproquest_cross%3E15741821%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=13635119&rft_id=info:pmid/&rft_els_id=0141113695000380&rfr_iscdi=true |