Early B‐Cell Factor (EBF) Down‐Regulates Immunoglobulin Heavy Chain Intron Enhancer Function in a Plasmacytoma Cell Line
The immunoglobulin heavy chain intron enhancer contains two potential binding sites for early B‐cell factor (EBF). To investigate the functional properties of these, EBF was expressed in the EBF non‐expressing S194 plasmacytoma cell line and found to down‐regulate the activity of a co‐transfected im...
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| Veröffentlicht in: | Scandinavian journal of immunology 1996-07, Vol.44 (1), p.89-92 |
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| container_title | Scandinavian journal of immunology |
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| creator | ÅKERBLAD, P. SIGVARDSSON, M. LEANDERSON, T. |
| description | The immunoglobulin heavy chain intron enhancer contains two potential binding sites for early B‐cell factor (EBF). To investigate the functional properties of these, EBF was expressed in the EBF non‐expressing S194 plasmacytoma cell line and found to down‐regulate the activity of a co‐transfected immunoglobulin heavy chain intron enhancer reporter construct. The expression of an unrelated reporter construct was unaltered. Dividing the immunoglobulin heavy chain intron enhancer into two subregions showed that the EBF mediated down‐regulation of expression was mediated by at least two independent sites. These data indicate a role for EBF in the regulation of immunoglobulin gene expression. |
| doi_str_mv | 10.1046/j.1365-3083.1996.d01-283.x |
| format | Article |
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To investigate the functional properties of these, EBF was expressed in the EBF non‐expressing S194 plasmacytoma cell line and found to down‐regulate the activity of a co‐transfected immunoglobulin heavy chain intron enhancer reporter construct. The expression of an unrelated reporter construct was unaltered. Dividing the immunoglobulin heavy chain intron enhancer into two subregions showed that the EBF mediated down‐regulation of expression was mediated by at least two independent sites. These data indicate a role for EBF in the regulation of immunoglobulin gene expression.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>DNA-Binding Proteins - pharmacology</subject><subject>Enhancer Elements, Genetic - immunology</subject><subject>Genes, Immunoglobulin - drug effects</subject><subject>Genes, Immunoglobulin - immunology</subject><subject>Immunoglobulin Heavy Chains - drug effects</subject><subject>Immunoglobulin Heavy Chains - genetics</subject><subject>Introns - drug effects</subject><subject>Introns - immunology</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Plasmacytoma - genetics</subject><subject>Plasmacytoma - immunology</subject><subject>Trans-Activators - pharmacology</subject><subject>Tumor Cells, Cultured</subject><issn>0300-9475</issn><issn>1365-3083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkMtO3DAUhq2qCKa0j4BksahgkcGOL4lZVILpTBk0EqiXteU4DmTk2NROgEhd9BH6jH2SepgR-65sn-8_51gfAMcYTTGi_Gw9xYSzjKCSTLEQfFojnOXp8fwGTF7RWzBBBKFM0IIdgHcxrhHCJC_IPtgvuSC5KCbg11wFO8LLv7__zIy1cKF07wM8mV8uTuFn_-QS-GruBqt6E-Gy6wbn76yvBts6eGXU4whn9yrdl64P3sG5u1dOmwAXg9N9myqJKXhrVeyUHnvfKfiyaNU68x7sNcpG82F3HoIfi_n32VW2uvmynF2sMk24EFlVVqpoOMW54rhkusxVbRpMjSYlyjVnWtO8qCmipGGCNobxStdNilSaKdqQQ_BxO_ch-J-Dib3s2qjTL5QzfogSs6IoKeMpeL4N6uBjDKaRD6HtVBglRnKjXq7lxq_c-JUb9TKpl0m9fE7NR7stQ9WZ-rV15zrxT1v-1Foz_sdk-e16iYkg_wDgp5Y8</recordid><startdate>199607</startdate><enddate>199607</enddate><creator>ÅKERBLAD, P.</creator><creator>SIGVARDSSON, M.</creator><creator>LEANDERSON, T.</creator><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7TM</scope><scope>H94</scope></search><sort><creationdate>199607</creationdate><title>Early B‐Cell Factor (EBF) Down‐Regulates Immunoglobulin Heavy Chain Intron Enhancer Function in a Plasmacytoma Cell Line</title><author>ÅKERBLAD, P. ; SIGVARDSSON, M. ; LEANDERSON, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3699-b8ba7f6412a6185c82adef14ec3802c65cc427d4043f594fe56bcdfef1bc5a4f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>DNA-Binding Proteins - pharmacology</topic><topic>Enhancer Elements, Genetic - immunology</topic><topic>Genes, Immunoglobulin - drug effects</topic><topic>Genes, Immunoglobulin - immunology</topic><topic>Immunoglobulin Heavy Chains - drug effects</topic><topic>Immunoglobulin Heavy Chains - genetics</topic><topic>Introns - drug effects</topic><topic>Introns - immunology</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Plasmacytoma - genetics</topic><topic>Plasmacytoma - immunology</topic><topic>Trans-Activators - pharmacology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ÅKERBLAD, P.</creatorcontrib><creatorcontrib>SIGVARDSSON, M.</creatorcontrib><creatorcontrib>LEANDERSON, T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Scandinavian journal of immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ÅKERBLAD, P.</au><au>SIGVARDSSON, M.</au><au>LEANDERSON, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Early B‐Cell Factor (EBF) Down‐Regulates Immunoglobulin Heavy Chain Intron Enhancer Function in a Plasmacytoma Cell Line</atitle><jtitle>Scandinavian journal of immunology</jtitle><addtitle>Scand J Immunol</addtitle><date>1996-07</date><risdate>1996</risdate><volume>44</volume><issue>1</issue><spage>89</spage><epage>92</epage><pages>89-92</pages><issn>0300-9475</issn><eissn>1365-3083</eissn><abstract>The immunoglobulin heavy chain intron enhancer contains two potential binding sites for early B‐cell factor (EBF). To investigate the functional properties of these, EBF was expressed in the EBF non‐expressing S194 plasmacytoma cell line and found to down‐regulate the activity of a co‐transfected immunoglobulin heavy chain intron enhancer reporter construct. The expression of an unrelated reporter construct was unaltered. Dividing the immunoglobulin heavy chain intron enhancer into two subregions showed that the EBF mediated down‐regulation of expression was mediated by at least two independent sites. These data indicate a role for EBF in the regulation of immunoglobulin gene expression.</abstract><cop>Oxford, U.K. and Cambridge, USA</cop><pub>Blackwell Science Ltd</pub><pmid>8693297</pmid><doi>10.1046/j.1365-3083.1996.d01-283.x</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-9475 |
| ispartof | Scandinavian journal of immunology, 1996-07, Vol.44 (1), p.89-92 |
| issn | 0300-9475 1365-3083 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15778456 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Base Sequence DNA-Binding Proteins - pharmacology Enhancer Elements, Genetic - immunology Genes, Immunoglobulin - drug effects Genes, Immunoglobulin - immunology Immunoglobulin Heavy Chains - drug effects Immunoglobulin Heavy Chains - genetics Introns - drug effects Introns - immunology Mice Molecular Sequence Data Plasmacytoma - genetics Plasmacytoma - immunology Trans-Activators - pharmacology Tumor Cells, Cultured |
| title | Early B‐Cell Factor (EBF) Down‐Regulates Immunoglobulin Heavy Chain Intron Enhancer Function in a Plasmacytoma Cell Line |
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