Evidence That Calcineurin Accommodates an Active Site Binuclear Metal Center

Evidence That Calcineurin Accommodates an Active Site Binuclear Metal Center

In this report we provide data indicating that the iron and zinc ions of native bovine brain calcineurin are present as a binuclear metal cluster, albeit in a ligand environment different from that in PAPase. Furthermore, we show that dithionite reduction of the bound ferric ion leads to loss of act...

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Veröffentlicht in:Journal of the American Chemical Society 1995-10, Vol.117 (40), p.10147-10148
Hauptverfasser: Yu, Lian, Haddy, Alice, Rusnak, Frank
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creator Yu, Lian
Haddy, Alice
Rusnak, Frank
description In this report we provide data indicating that the iron and zinc ions of native bovine brain calcineurin are present as a binuclear metal cluster, albeit in a ligand environment different from that in PAPase. Furthermore, we show that dithionite reduction of the bound ferric ion leads to loss of activity which can be quantitatively recovered upon reoxidation, indicating that the oxidation state of bound iron is important for enzymatic function. (DBO)
doi_str_mv 10.1021/ja00145a040
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title Evidence That Calcineurin Accommodates an Active Site Binuclear Metal Center
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