Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts
We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by pro...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1990-01, Vol.87 (2), p.816-820 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Harkins, K.R. (University of Arizona, Tucson, AZ) Jefferson, R.A Kavanagh, T.A Bevan, M.W Galbraith, D.W |
| description | We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts. |
| doi_str_mv | 10.1073/pnas.87.2.816 |
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These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.87.2.816</identifier><identifier>PMID: 2300565</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Base Sequence ; Biological and medical sciences ; Cells ; Chimera ; Chlorophylls ; Cloning, Molecular ; DNA - genetics ; Flow Cytometry ; FOTOSINTESIS ; Fundamental and applied biological sciences. Psychology ; GENE ; Gene expression ; Gene Expression - drug effects ; Gene fusion ; GENES ; GENETIC TRANSFORMATION ; Guard cells ; HERBICIDAS ; HERBICIDE ; HERBICIDES ; Herbicides - pharmacology ; Mesophyll ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mosaic Viruses ; Nicotiana - genetics ; NICOTIANA TABACUM ; NORFLURAZON ; PHOTOSYNTHESE ; PHOTOSYNTHESIS ; Plants ; Plants - genetics ; Plants, Toxic ; PLASMIDE ; PLASMIDIOS ; PLASMIDS ; PROTOPLASTE ; PROTOPLASTO ; PROTOPLASTS ; Protoplasts - metabolism ; Pyridazines - pharmacology ; Transcription, Genetic ; Transfection ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1990-01, Vol.87 (2), p.816-820</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c633t-587e0fef9a3eb34f23eecf86bcd7ad1487274490cb580d8c8d22dcbcba097cd53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/87/2.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2353602$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2353602$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,4010,27900,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6904273$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2300565$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harkins, K.R. (University of Arizona, Tucson, AZ)</creatorcontrib><creatorcontrib>Jefferson, R.A</creatorcontrib><creatorcontrib>Kavanagh, T.A</creatorcontrib><creatorcontrib>Bevan, M.W</creatorcontrib><creatorcontrib>Galbraith, D.W</creatorcontrib><title>Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cells</subject><subject>Chimera</subject><subject>Chlorophylls</subject><subject>Cloning, Molecular</subject><subject>DNA - genetics</subject><subject>Flow Cytometry</subject><subject>FOTOSINTESIS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>Gene expression</subject><subject>Gene Expression - drug effects</subject><subject>Gene fusion</subject><subject>GENES</subject><subject>GENETIC TRANSFORMATION</subject><subject>Guard cells</subject><subject>HERBICIDAS</subject><subject>HERBICIDE</subject><subject>HERBICIDES</subject><subject>Herbicides - pharmacology</subject><subject>Mesophyll</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mosaic Viruses</subject><subject>Nicotiana - genetics</subject><subject>NICOTIANA TABACUM</subject><subject>NORFLURAZON</subject><subject>PHOTOSYNTHESE</subject><subject>PHOTOSYNTHESIS</subject><subject>Plants</subject><subject>Plants - genetics</subject><subject>Plants, Toxic</subject><subject>PLASMIDE</subject><subject>PLASMIDIOS</subject><subject>PLASMIDS</subject><subject>PROTOPLASTE</subject><subject>PROTOPLASTO</subject><subject>PROTOPLASTS</subject><subject>Protoplasts - metabolism</subject><subject>Pyridazines - pharmacology</subject><subject>Transcription, Genetic</subject><subject>Transfection</subject><subject>TRANSFORMACION GENETICA</subject><subject>TRANSFORMATION GENETIQUE</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kb1v1DAYhyMEKkdhZEEgeQC2HI4dx7bEgqryIVVigM6W49h3rnJ28OtDvY0_HYdLj7IweXie3_vht6qeN3jdYE7fTUHDWvA1WYume1CtGiybumslflitMCa8Fi1pH1dPAG4wxpIJfFadEYox69iq-nV5OyUL4GNA0aFpG3OEQ8hbCx7qZEed7YA2Nljk9rMFyAMqiZy8mVF_QMaOI8qHySIfUE46QPG9QdOoQwakw3ACzv4JTam0KRgyPK0eOT2Cfba859X1x8vvF5_rq6-fvlx8uKpNR2mumeAWO-ukpranrSPUWuNE15uB66FpBSe8LUubviw4CCMGQgbTm15jyc3A6Hn1_lh32vc7OxgbykCjmpLf6XRQUXv1Lwl-qzbxp2KUMl7ib5d4ij_2ZX218zAvroONe1AN45wKOfepj6JJESBZd2rRYDUfTM0HU4IrosrBiv_q_lwne7lQ4a8XrsHo0ZVfNB5OWidxSzgt2ptFm6vf0bsuyu3HMdvbXLyX__H-4hvIMd0bhtEOk4JfHLHTUelNKoNcf5OYtExK-hsxxM0L</recordid><startdate>199001</startdate><enddate>199001</enddate><creator>Harkins, K.R. 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(University of Arizona, Tucson, AZ) ; Jefferson, R.A ; Kavanagh, T.A ; Bevan, M.W ; Galbraith, D.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c633t-587e0fef9a3eb34f23eecf86bcd7ad1487274490cb580d8c8d22dcbcba097cd53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cells</topic><topic>Chimera</topic><topic>Chlorophylls</topic><topic>Cloning, Molecular</topic><topic>DNA - genetics</topic><topic>Flow Cytometry</topic><topic>FOTOSINTESIS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>Gene expression</topic><topic>Gene Expression - drug effects</topic><topic>Gene fusion</topic><topic>GENES</topic><topic>GENETIC TRANSFORMATION</topic><topic>Guard cells</topic><topic>HERBICIDAS</topic><topic>HERBICIDE</topic><topic>HERBICIDES</topic><topic>Herbicides - pharmacology</topic><topic>Mesophyll</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mosaic Viruses</topic><topic>Nicotiana - genetics</topic><topic>NICOTIANA TABACUM</topic><topic>NORFLURAZON</topic><topic>PHOTOSYNTHESE</topic><topic>PHOTOSYNTHESIS</topic><topic>Plants</topic><topic>Plants - genetics</topic><topic>Plants, Toxic</topic><topic>PLASMIDE</topic><topic>PLASMIDIOS</topic><topic>PLASMIDS</topic><topic>PROTOPLASTE</topic><topic>PROTOPLASTO</topic><topic>PROTOPLASTS</topic><topic>Protoplasts - metabolism</topic><topic>Pyridazines - pharmacology</topic><topic>Transcription, Genetic</topic><topic>Transfection</topic><topic>TRANSFORMACION GENETICA</topic><topic>TRANSFORMATION GENETIQUE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harkins, K.R. 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(University of Arizona, Tucson, AZ)</au><au>Jefferson, R.A</au><au>Kavanagh, T.A</au><au>Bevan, M.W</au><au>Galbraith, D.W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1990-01</date><risdate>1990</risdate><volume>87</volume><issue>2</issue><spage>816</spage><epage>820</epage><pages>816-820</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2300565</pmid><doi>10.1073/pnas.87.2.816</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1990-01, Vol.87 (2), p.816-820 |
| issn | 0027-8424 1091-6490 |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Base Sequence Biological and medical sciences Cells Chimera Chlorophylls Cloning, Molecular DNA - genetics Flow Cytometry FOTOSINTESIS Fundamental and applied biological sciences. Psychology GENE Gene expression Gene Expression - drug effects Gene fusion GENES GENETIC TRANSFORMATION Guard cells HERBICIDAS HERBICIDE HERBICIDES Herbicides - pharmacology Mesophyll Molecular and cellular biology Molecular genetics Molecular Sequence Data Mosaic Viruses Nicotiana - genetics NICOTIANA TABACUM NORFLURAZON PHOTOSYNTHESE PHOTOSYNTHESIS Plants Plants - genetics Plants, Toxic PLASMIDE PLASMIDIOS PLASMIDS PROTOPLASTE PROTOPLASTO PROTOPLASTS Protoplasts - metabolism Pyridazines - pharmacology Transcription, Genetic Transfection TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE |
| title | Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts |
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