Inclusion of an upstream transcriptional terminator in phage display vectors abolishes background expression of toxic fusions with coat protein g3p
Expression of toxic gene products affects bacterial cell growth and phage display, causing a strong selection against plasmid maintenance and integrity. During phage propagation steps, in particular, phagemid instability can dramatically affect diversity of antibody libraries or even lead to the del...
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| Veröffentlicht in: | Gene 1996-10, Vol.178 (1), p.71-74 |
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| container_end_page | 74 |
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| container_issue | 1 |
| container_start_page | 71 |
| container_title | Gene |
| container_volume | 178 |
| creator | Krebber, Anke Burmester, Jörg Plückthun, Andreas |
| description | Expression of toxic gene products affects bacterial cell growth and phage display, causing a strong selection against plasmid maintenance and integrity. During phage propagation steps, in particular, phagemid instability can dramatically affect diversity of antibody libraries or even lead to the deletion of antibody genes. We constructed a modified phage display vector by introducing a strong transcriptional terminator upstream of the
lac promoter, which, together with glucose suppression of its CAP-dependent activation, very efficiently represses product formation before induction. |
| doi_str_mv | 10.1016/0378-1119(96)00337-X |
| format | Article |
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lac promoter, which, together with glucose suppression of its CAP-dependent activation, very efficiently represses product formation before induction.</description><subject>Antibody libraries</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacteriophage M13 - genetics</subject><subject>Base Sequence</subject><subject>Capsid Proteins</subject><subject>Cloning, Molecular - methods</subject><subject>DNA, Recombinant</subject><subject>DNA-Binding Proteins - genetics</subject><subject>E. coli</subject><subject>Escherichia coli Proteins</subject><subject>Filamentous phage</subject><subject>Gene Expression Regulation</subject><subject>Genetic Vectors</subject><subject>Immunoglobulin Fragments - genetics</subject><subject>Lac Repressors</subject><subject>Molecular Sequence Data</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant antibodies</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - pharmacology</subject><subject>Repressor Proteins - genetics</subject><subject>Terminator Regions, Genetic</subject><subject>Viral Fusion Proteins - genetics</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1v1DAQtRCoLIV_AJJPCA4BO14n9gUJVXxUqsQFpN4sxx7vGpI4eJzS_g7-MF526RFfrJn35j3NG0Kec_aGM969ZaJXDedcv9Lda8aE6JvrB2TDVa-bWqqHZHNPeUyeIH5n9UnZnpEzpVuu9HZDfl_OblwxppmmQO1M1wVLBjvRku2MLselVNCOtECe4mxLyjTOdNnbHVAfcRntHb0BV_tI7ZDGiHtAOlj3Y5fTOnsKt0sG_GdR0m10NPy1RPorlj11yRa65FSgCu_E8pQ8CnZEeHb6z8m3jx--Xnxurr58urx4f9U4oVRpOunawUvpwzBozlQrvQ1s4LXTMZBcSSd6rj1A2CohdAfScSnCYIPXVvXinLw86lbvnytgMVNEB-NoZ0grGi77ru2ZrsTtkehyQswQzJLjZPOd4cwcbmEOQZtD0EbX4nALc13HXpz012ECfz90Cr_i74441CVvImSDLsLswMdcAzU-xf8b_AETPJ1_</recordid><startdate>19961031</startdate><enddate>19961031</enddate><creator>Krebber, Anke</creator><creator>Burmester, Jörg</creator><creator>Plückthun, Andreas</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>19961031</creationdate><title>Inclusion of an upstream transcriptional terminator in phage display vectors abolishes background expression of toxic fusions with coat protein g3p</title><author>Krebber, Anke ; Burmester, Jörg ; Plückthun, Andreas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-65c2bd55dfbb910825daf0b155d60e5185c3719deef483396e5c153fbafd9a873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Antibody libraries</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacteriophage M13 - genetics</topic><topic>Base Sequence</topic><topic>Capsid Proteins</topic><topic>Cloning, Molecular - methods</topic><topic>DNA, Recombinant</topic><topic>DNA-Binding Proteins - genetics</topic><topic>E. coli</topic><topic>Escherichia coli Proteins</topic><topic>Filamentous phage</topic><topic>Gene Expression Regulation</topic><topic>Genetic Vectors</topic><topic>Immunoglobulin Fragments - genetics</topic><topic>Lac Repressors</topic><topic>Molecular Sequence Data</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant antibodies</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - pharmacology</topic><topic>Repressor Proteins - genetics</topic><topic>Terminator Regions, Genetic</topic><topic>Viral Fusion Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krebber, Anke</creatorcontrib><creatorcontrib>Burmester, Jörg</creatorcontrib><creatorcontrib>Plückthun, Andreas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krebber, Anke</au><au>Burmester, Jörg</au><au>Plückthun, Andreas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inclusion of an upstream transcriptional terminator in phage display vectors abolishes background expression of toxic fusions with coat protein g3p</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1996-10-31</date><risdate>1996</risdate><volume>178</volume><issue>1</issue><spage>71</spage><epage>74</epage><pages>71-74</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>Expression of toxic gene products affects bacterial cell growth and phage display, causing a strong selection against plasmid maintenance and integrity. During phage propagation steps, in particular, phagemid instability can dramatically affect diversity of antibody libraries or even lead to the deletion of antibody genes. We constructed a modified phage display vector by introducing a strong transcriptional terminator upstream of the
lac promoter, which, together with glucose suppression of its CAP-dependent activation, very efficiently represses product formation before induction.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8921894</pmid><doi>10.1016/0378-1119(96)00337-X</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1996-10, Vol.178 (1), p.71-74 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15762709 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Antibody libraries Bacterial Proteins - genetics Bacteriophage M13 - genetics Base Sequence Capsid Proteins Cloning, Molecular - methods DNA, Recombinant DNA-Binding Proteins - genetics E. coli Escherichia coli Proteins Filamentous phage Gene Expression Regulation Genetic Vectors Immunoglobulin Fragments - genetics Lac Repressors Molecular Sequence Data Promoter Regions, Genetic Recombinant antibodies Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - pharmacology Repressor Proteins - genetics Terminator Regions, Genetic Viral Fusion Proteins - genetics |
| title | Inclusion of an upstream transcriptional terminator in phage display vectors abolishes background expression of toxic fusions with coat protein g3p |
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