Pathways of signal transduction in teleost nonspecific cytotoxic cells
In the present study evidence is presented that both a putative “receptor” binding monoclonal antibody (mAb) and the calcium ionophore A23187, either singly or together, increase receptor expression and lysis of IM-9 target cells by catfish NCC. NCC activity against IM-9 target cells was increased 5...
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| Veröffentlicht in: | Developmental and comparative immunology 1990, Vol.14 (3), p.295-304 |
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| creator | Evans, Donald L. Harris, David T. Staton, Donna L. Jaso-Friedmann, Liliana |
| description | In the present study evidence is presented that both a putative “receptor” binding monoclonal antibody (mAb) and the calcium ionophore A23187, either singly or together, increase receptor expression and lysis of IM-9 target cells by catfish NCC. NCC activity against IM-9 target cells was increased 55% after 1 h mAb treatment. Receptor expression determined by flow cytometry increased 95% following 18-h treatment. A23187 treatment of NCC produced greater than 200% increases in receptor expression. Combined treatments of NCC with 10
-4 M A23187 and 10
-7 M PMA however augmented receptor expression only 22% above that produced by A23187 alone. MAb and A23187 comodulated cytotoxicity by a 65% increase over ionophore treatment alone. MAb and 10
-10 M PMA comodulation produced only 10.6% increases in cytotoxicity compared to mAb. These data demonstrate that the moiety on NCC recognized by 5C6 may provide an activation signal for increased cytotoxicity and receptor expression. Calcium ionophore, either singly or together with mAb, provided an even stronger activation signal for increased lysis and receptor expression. |
| doi_str_mv | 10.1016/0145-305X(90)90020-F |
| format | Article |
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-4 M A23187 and 10
-7 M PMA however augmented receptor expression only 22% above that produced by A23187 alone. MAb and A23187 comodulated cytotoxicity by a 65% increase over ionophore treatment alone. MAb and 10
-10 M PMA comodulation produced only 10.6% increases in cytotoxicity compared to mAb. These data demonstrate that the moiety on NCC recognized by 5C6 may provide an activation signal for increased cytotoxicity and receptor expression. Calcium ionophore, either singly or together with mAb, provided an even stronger activation signal for increased lysis and receptor expression.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/0145-305X(90)90020-F</identifier><identifier>PMID: 2210008</identifier><identifier>CODEN: DCIMDQ</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>A23187 modulation ; Analysis of the immune response. Humoral and cellular immunity ; Animals ; Antibodies, Monoclonal ; Biological and medical sciences ; Calcimycin - pharmacology ; Catfishes - immunology ; Cell Cycle ; Cytotoxicity, Immunologic - drug effects ; Cytotoxicity, Immunologic - physiology ; Female ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Gene Expression Regulation ; Humans ; Ictalurus punctatus ; Immunobiology ; Killer Cells, Natural - immunology ; Male ; monoclonal antibody ; NCC ; Organs and cells involved in the immune response ; Rats ; receptor modulation ; Receptors, Immunologic - biosynthesis ; Signal transduction ; Signal Transduction - immunology ; Tetradecanoylphorbol Acetate - pharmacology</subject><ispartof>Developmental and comparative immunology, 1990, Vol.14 (3), p.295-304</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-ea997595aed495c5eb19600204d5c8fe66928591a7098065b488c29dcd531ac53</citedby><cites>FETCH-LOGICAL-c418t-ea997595aed495c5eb19600204d5c8fe66928591a7098065b488c29dcd531ac53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0145305X9090020F$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19653177$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2210008$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Evans, Donald L.</creatorcontrib><creatorcontrib>Harris, David T.</creatorcontrib><creatorcontrib>Staton, Donna L.</creatorcontrib><creatorcontrib>Jaso-Friedmann, Liliana</creatorcontrib><title>Pathways of signal transduction in teleost nonspecific cytotoxic cells</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>In the present study evidence is presented that both a putative “receptor” binding monoclonal antibody (mAb) and the calcium ionophore A23187, either singly or together, increase receptor expression and lysis of IM-9 target cells by catfish NCC. NCC activity against IM-9 target cells was increased 55% after 1 h mAb treatment. Receptor expression determined by flow cytometry increased 95% following 18-h treatment. A23187 treatment of NCC produced greater than 200% increases in receptor expression. Combined treatments of NCC with 10
-4 M A23187 and 10
-7 M PMA however augmented receptor expression only 22% above that produced by A23187 alone. MAb and A23187 comodulated cytotoxicity by a 65% increase over ionophore treatment alone. MAb and 10
-10 M PMA comodulation produced only 10.6% increases in cytotoxicity compared to mAb. These data demonstrate that the moiety on NCC recognized by 5C6 may provide an activation signal for increased cytotoxicity and receptor expression. Calcium ionophore, either singly or together with mAb, provided an even stronger activation signal for increased lysis and receptor expression.</description><subject>A23187 modulation</subject><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Calcimycin - pharmacology</subject><subject>Catfishes - immunology</subject><subject>Cell Cycle</subject><subject>Cytotoxicity, Immunologic - drug effects</subject><subject>Cytotoxicity, Immunologic - physiology</subject><subject>Female</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Gene Expression Regulation</subject><subject>Humans</subject><subject>Ictalurus punctatus</subject><subject>Immunobiology</subject><subject>Killer Cells, Natural - immunology</subject><subject>Male</subject><subject>monoclonal antibody</subject><subject>NCC</subject><subject>Organs and cells involved in the immune response</subject><subject>Rats</subject><subject>receptor modulation</subject><subject>Receptors, Immunologic - biosynthesis</subject><subject>Signal transduction</subject><subject>Signal Transduction - immunology</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFKxDAQhoMo67r6Bgq9KHqoJt0mTS6CLFaFBT0oeAvZdKqRbrN2UnXf3tYtejOXDMw3Pz8fIYeMnjPKxAVlKY-nlD-fKnqmKE1onG-RMZOZiimVapuMf5Fdsof4RrsnGR2RUZKwfh6T_MGE10-zxsiXEbqX2lRRaEyNRWuD83Xk6ihABR5DVPsaV2Bd6Wxk18EH_9VPUFW4T3ZKUyEcDP-EPOXXj7PbeH5_cze7msc2ZTLEYJTKuOIGilRxy2HBlOibpwW3sgQhVCK5YiajSlLBF6mUNlGFLfiUGcunE3KyyV01_r0FDHrpsG9gavAtasYzwRIlOjDdgLbxiA2UetW4pWnWmlHd69O9G9270YrqH306786Ohvx2sYTi92jw1e2Ph71Ba6qyM2Ud_mUr0RXNso673HDQyfhw0Gi0DmoLhWvABl1493-Rbwjki7E</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Evans, Donald L.</creator><creator>Harris, David T.</creator><creator>Staton, Donna L.</creator><creator>Jaso-Friedmann, Liliana</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>1990</creationdate><title>Pathways of signal transduction in teleost nonspecific cytotoxic cells</title><author>Evans, Donald L. ; Harris, David T. ; Staton, Donna L. ; Jaso-Friedmann, Liliana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-ea997595aed495c5eb19600204d5c8fe66928591a7098065b488c29dcd531ac53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>A23187 modulation</topic><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Calcimycin - pharmacology</topic><topic>Catfishes - immunology</topic><topic>Cell Cycle</topic><topic>Cytotoxicity, Immunologic - drug effects</topic><topic>Cytotoxicity, Immunologic - physiology</topic><topic>Female</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Gene Expression Regulation</topic><topic>Humans</topic><topic>Ictalurus punctatus</topic><topic>Immunobiology</topic><topic>Killer Cells, Natural - immunology</topic><topic>Male</topic><topic>monoclonal antibody</topic><topic>NCC</topic><topic>Organs and cells involved in the immune response</topic><topic>Rats</topic><topic>receptor modulation</topic><topic>Receptors, Immunologic - biosynthesis</topic><topic>Signal transduction</topic><topic>Signal Transduction - immunology</topic><topic>Tetradecanoylphorbol Acetate - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Evans, Donald L.</creatorcontrib><creatorcontrib>Harris, David T.</creatorcontrib><creatorcontrib>Staton, Donna L.</creatorcontrib><creatorcontrib>Jaso-Friedmann, Liliana</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Evans, Donald L.</au><au>Harris, David T.</au><au>Staton, Donna L.</au><au>Jaso-Friedmann, Liliana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pathways of signal transduction in teleost nonspecific cytotoxic cells</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>1990</date><risdate>1990</risdate><volume>14</volume><issue>3</issue><spage>295</spage><epage>304</epage><pages>295-304</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><coden>DCIMDQ</coden><abstract>In the present study evidence is presented that both a putative “receptor” binding monoclonal antibody (mAb) and the calcium ionophore A23187, either singly or together, increase receptor expression and lysis of IM-9 target cells by catfish NCC. NCC activity against IM-9 target cells was increased 55% after 1 h mAb treatment. Receptor expression determined by flow cytometry increased 95% following 18-h treatment. A23187 treatment of NCC produced greater than 200% increases in receptor expression. Combined treatments of NCC with 10
-4 M A23187 and 10
-7 M PMA however augmented receptor expression only 22% above that produced by A23187 alone. MAb and A23187 comodulated cytotoxicity by a 65% increase over ionophore treatment alone. MAb and 10
-10 M PMA comodulation produced only 10.6% increases in cytotoxicity compared to mAb. These data demonstrate that the moiety on NCC recognized by 5C6 may provide an activation signal for increased cytotoxicity and receptor expression. Calcium ionophore, either singly or together with mAb, provided an even stronger activation signal for increased lysis and receptor expression.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>2210008</pmid><doi>10.1016/0145-305X(90)90020-F</doi><tpages>10</tpages></addata></record> |
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| ispartof | Developmental and comparative immunology, 1990, Vol.14 (3), p.295-304 |
| issn | 0145-305X 1879-0089 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | A23187 modulation Analysis of the immune response. Humoral and cellular immunity Animals Antibodies, Monoclonal Biological and medical sciences Calcimycin - pharmacology Catfishes - immunology Cell Cycle Cytotoxicity, Immunologic - drug effects Cytotoxicity, Immunologic - physiology Female Freshwater Fundamental and applied biological sciences. Psychology Fundamental immunology Gene Expression Regulation Humans Ictalurus punctatus Immunobiology Killer Cells, Natural - immunology Male monoclonal antibody NCC Organs and cells involved in the immune response Rats receptor modulation Receptors, Immunologic - biosynthesis Signal transduction Signal Transduction - immunology Tetradecanoylphorbol Acetate - pharmacology |
| title | Pathways of signal transduction in teleost nonspecific cytotoxic cells |
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