Development of cholinergic markers in the neuroblastoma, N1E-115
The neuroblastoma, N1E‐115, was grown for 9 days after subcultivation. The development of acetylcholinestrase (AChE), choline acetyltransferase (CAT), QNB‐binding, choline uptake, and acetylcholine release was measured on days 1, 3, 6, and 9. In paralell experiments the irreversible AChE inhibitor s...
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| Veröffentlicht in: | Journal of neuroscience research 1990-09, Vol.27 (1), p.99-105 |
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| creator | Kumar, U. Sellström, Å. |
| description | The neuroblastoma, N1E‐115, was grown for 9 days after subcultivation. The development of acetylcholinestrase (AChE), choline acetyltransferase (CAT), QNB‐binding, choline uptake, and acetylcholine release was measured on days 1, 3, 6, and 9. In paralell experiments the irreversible AChE inhibitor soman was added to neuroblastoma on day 1 and the development of the above parameters except for release, was followed.
AChE activity in the normal cells was found to develop also after confluency, whereas CAT activity and QNB‐binding followed the development of most of the cellular proteins, i.e., ceased to develop at confluency. Both choline uptake and acetylcholine release appeared independent of cellular development.
In the soman‐treated cells the development of AChE was inhibited for up to 6 days and thereafter developed with the sama rate as in the normal cells. CAT and QNB‐binding developed as in the normal cells, but at a significantly reduced level. The development of choline uptake was not significantly different in soman‐treated and normal neuroblastoma.
It is concluded that the development of the cholinergic marker QNB‐binding is intimately associated with that of the “presynaptic” marker CAT, whereas the development of AChE seems to be unrelated to these cholinergic parameters. The choline transport and the acetylcholine release seem to be equally well expressed on all the days studied. |
| doi_str_mv | 10.1002/jnr.490270115 |
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AChE activity in the normal cells was found to develop also after confluency, whereas CAT activity and QNB‐binding followed the development of most of the cellular proteins, i.e., ceased to develop at confluency. Both choline uptake and acetylcholine release appeared independent of cellular development.
In the soman‐treated cells the development of AChE was inhibited for up to 6 days and thereafter developed with the sama rate as in the normal cells. CAT and QNB‐binding developed as in the normal cells, but at a significantly reduced level. The development of choline uptake was not significantly different in soman‐treated and normal neuroblastoma.
It is concluded that the development of the cholinergic marker QNB‐binding is intimately associated with that of the “presynaptic” marker CAT, whereas the development of AChE seems to be unrelated to these cholinergic parameters. The choline transport and the acetylcholine release seem to be equally well expressed on all the days studied.</description><identifier>ISSN: 0360-4012</identifier><identifier>EISSN: 1097-4547</identifier><identifier>DOI: 10.1002/jnr.490270115</identifier><identifier>PMID: 2254960</identifier><identifier>CODEN: JNREDK</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>acetylcholine ; Acetylcholine - secretion ; Acetylcholinesterase - analysis ; AChE ; Animals ; Biological and medical sciences ; Biomarkers ; CAT ; Cell Division - drug effects ; Cell physiology ; choline ; Choline - metabolism ; Choline O-Acetyltransferase - analysis ; Down-Regulation - drug effects ; Fundamental and applied biological sciences. Psychology ; Mice ; Molecular and cellular biology ; muscarinic receptor ; muscarinic receptor; choline ; Neuroblastoma - pathology ; Neurotransmission ; Quinuclidinyl Benzilate - metabolism ; Receptors, Muscarinic - metabolism ; release of ; release of; soman ; soman ; Soman - metabolism ; Soman - pharmacology ; transport of ; transport of; acetylcholine ; Tumor Cells, Cultured - chemistry ; Tumor Cells, Cultured - drug effects</subject><ispartof>Journal of neuroscience research, 1990-09, Vol.27 (1), p.99-105</ispartof><rights>Copyright © 1990 Wiley‐Liss, Inc.</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4355-977175680603e7488aa9f40368f06f3510081cbd4a5e84dcdda5fe4de1f581b13</citedby><cites>FETCH-LOGICAL-c4355-977175680603e7488aa9f40368f06f3510081cbd4a5e84dcdda5fe4de1f581b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjnr.490270115$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjnr.490270115$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19740978$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2254960$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumar, U.</creatorcontrib><creatorcontrib>Sellström, Å.</creatorcontrib><title>Development of cholinergic markers in the neuroblastoma, N1E-115</title><title>Journal of neuroscience research</title><addtitle>J. Neurosci. Res</addtitle><description>The neuroblastoma, N1E‐115, was grown for 9 days after subcultivation. The development of acetylcholinestrase (AChE), choline acetyltransferase (CAT), QNB‐binding, choline uptake, and acetylcholine release was measured on days 1, 3, 6, and 9. In paralell experiments the irreversible AChE inhibitor soman was added to neuroblastoma on day 1 and the development of the above parameters except for release, was followed.
AChE activity in the normal cells was found to develop also after confluency, whereas CAT activity and QNB‐binding followed the development of most of the cellular proteins, i.e., ceased to develop at confluency. Both choline uptake and acetylcholine release appeared independent of cellular development.
In the soman‐treated cells the development of AChE was inhibited for up to 6 days and thereafter developed with the sama rate as in the normal cells. CAT and QNB‐binding developed as in the normal cells, but at a significantly reduced level. The development of choline uptake was not significantly different in soman‐treated and normal neuroblastoma.
It is concluded that the development of the cholinergic marker QNB‐binding is intimately associated with that of the “presynaptic” marker CAT, whereas the development of AChE seems to be unrelated to these cholinergic parameters. The choline transport and the acetylcholine release seem to be equally well expressed on all the days studied.</description><subject>acetylcholine</subject><subject>Acetylcholine - secretion</subject><subject>Acetylcholinesterase - analysis</subject><subject>AChE</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers</subject><subject>CAT</subject><subject>Cell Division - drug effects</subject><subject>Cell physiology</subject><subject>choline</subject><subject>Choline - metabolism</subject><subject>Choline O-Acetyltransferase - analysis</subject><subject>Down-Regulation - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>muscarinic receptor</subject><subject>muscarinic receptor; choline</subject><subject>Neuroblastoma - pathology</subject><subject>Neurotransmission</subject><subject>Quinuclidinyl Benzilate - metabolism</subject><subject>Receptors, Muscarinic - metabolism</subject><subject>release of</subject><subject>release of; soman</subject><subject>soman</subject><subject>Soman - metabolism</subject><subject>Soman - pharmacology</subject><subject>transport of</subject><subject>transport of; acetylcholine</subject><subject>Tumor Cells, Cultured - chemistry</subject><subject>Tumor Cells, Cultured - drug effects</subject><issn>0360-4012</issn><issn>1097-4547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtP3DAURi1URIcpS5ZI2ZQVodexHTs7Wh4DCKUVAlXqxvI41yWQx2BnePx7jCYauurKi3v83e8eQnYpHFKA7Nt95w95AZkESsUGmVAoZMoFl5_IBFgOKQeafSbbIdwDQFEItkW2skzwIocJOTrBJ2z6RYvdkPQusXd9U3fo_9Y2aY1_QB-SukuGO0w6XPp-3pgw9K05SEp6msaNX8imM03AnfGdktuz05vj8_Tq5-zi-PtVajkTIi2kpFLkCnJgKLlSxhSOx37KQe6YiKcoaucVNwIVr2xVGeGQV0idUHRO2ZTsr3IXvn9cYhh0WweLTWM67JdBUyE5k8AimK5A6_sQPDq98HU85VVT0O_GdDSm18YivzcGL-ctVmt6VBTnX8e5CdY0zpvO1uEjtJA8GleRkyvuuW7w9f9L9WV5_W-DsXEdBnxZ_4z2dS6ZFPp3OdN_Ln_8UmV5om_YG27ckQQ</recordid><startdate>199009</startdate><enddate>199009</enddate><creator>Kumar, U.</creator><creator>Sellström, Å.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>199009</creationdate><title>Development of cholinergic markers in the neuroblastoma, N1E-115</title><author>Kumar, U. ; Sellström, Å.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4355-977175680603e7488aa9f40368f06f3510081cbd4a5e84dcdda5fe4de1f581b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>acetylcholine</topic><topic>Acetylcholine - secretion</topic><topic>Acetylcholinesterase - analysis</topic><topic>AChE</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers</topic><topic>CAT</topic><topic>Cell Division - drug effects</topic><topic>Cell physiology</topic><topic>choline</topic><topic>Choline - metabolism</topic><topic>Choline O-Acetyltransferase - analysis</topic><topic>Down-Regulation - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>muscarinic receptor</topic><topic>muscarinic receptor; choline</topic><topic>Neuroblastoma - pathology</topic><topic>Neurotransmission</topic><topic>Quinuclidinyl Benzilate - metabolism</topic><topic>Receptors, Muscarinic - metabolism</topic><topic>release of</topic><topic>release of; soman</topic><topic>soman</topic><topic>Soman - metabolism</topic><topic>Soman - pharmacology</topic><topic>transport of</topic><topic>transport of; acetylcholine</topic><topic>Tumor Cells, Cultured - chemistry</topic><topic>Tumor Cells, Cultured - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, U.</creatorcontrib><creatorcontrib>Sellström, Å.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, U.</au><au>Sellström, Å.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of cholinergic markers in the neuroblastoma, N1E-115</atitle><jtitle>Journal of neuroscience research</jtitle><addtitle>J. Neurosci. Res</addtitle><date>1990-09</date><risdate>1990</risdate><volume>27</volume><issue>1</issue><spage>99</spage><epage>105</epage><pages>99-105</pages><issn>0360-4012</issn><eissn>1097-4547</eissn><coden>JNREDK</coden><abstract>The neuroblastoma, N1E‐115, was grown for 9 days after subcultivation. The development of acetylcholinestrase (AChE), choline acetyltransferase (CAT), QNB‐binding, choline uptake, and acetylcholine release was measured on days 1, 3, 6, and 9. In paralell experiments the irreversible AChE inhibitor soman was added to neuroblastoma on day 1 and the development of the above parameters except for release, was followed.
AChE activity in the normal cells was found to develop also after confluency, whereas CAT activity and QNB‐binding followed the development of most of the cellular proteins, i.e., ceased to develop at confluency. Both choline uptake and acetylcholine release appeared independent of cellular development.
In the soman‐treated cells the development of AChE was inhibited for up to 6 days and thereafter developed with the sama rate as in the normal cells. CAT and QNB‐binding developed as in the normal cells, but at a significantly reduced level. The development of choline uptake was not significantly different in soman‐treated and normal neuroblastoma.
It is concluded that the development of the cholinergic marker QNB‐binding is intimately associated with that of the “presynaptic” marker CAT, whereas the development of AChE seems to be unrelated to these cholinergic parameters. The choline transport and the acetylcholine release seem to be equally well expressed on all the days studied.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2254960</pmid><doi>10.1002/jnr.490270115</doi><tpages>7</tpages></addata></record> |
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| subjects | acetylcholine Acetylcholine - secretion Acetylcholinesterase - analysis AChE Animals Biological and medical sciences Biomarkers CAT Cell Division - drug effects Cell physiology choline Choline - metabolism Choline O-Acetyltransferase - analysis Down-Regulation - drug effects Fundamental and applied biological sciences. Psychology Mice Molecular and cellular biology muscarinic receptor muscarinic receptor choline Neuroblastoma - pathology Neurotransmission Quinuclidinyl Benzilate - metabolism Receptors, Muscarinic - metabolism release of release of soman soman Soman - metabolism Soman - pharmacology transport of transport of acetylcholine Tumor Cells, Cultured - chemistry Tumor Cells, Cultured - drug effects |
| title | Development of cholinergic markers in the neuroblastoma, N1E-115 |
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