The synthesis of soluble polymer-ligand complexes for affinity precipitation studies
Protein A has been covalently coupled to a water soluble galactomannan bearing aldehyde groups, introduced by oxidation with D-galactose oxidase. The soluble conjugated has been used to form complexes with immunoglobulin G, precipitation being induced by the formation of polysaccharide-borate comple...
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| Veröffentlicht in: | Biotechnology techniques 1990, Vol.4 (1), p.67-71 |
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| container_end_page | 71 |
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| container_issue | 1 |
| container_start_page | 67 |
| container_title | Biotechnology techniques |
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| creator | Bradshaw, A. P. Sturgeon, R. J. |
| description | Protein A has been covalently coupled to a water soluble galactomannan bearing aldehyde groups, introduced by oxidation with D-galactose oxidase. The soluble conjugated has been used to form complexes with immunoglobulin G, precipitation being induced by the formation of polysaccharide-borate complexes. After recovery of the immunoglobulin, the polysaccharide-protein A conjugates can be re-cycled and used for further isolations. |
| doi_str_mv | 10.1007/BF00156613 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0951-208X |
| ispartof | Biotechnology techniques, 1990, Vol.4 (1), p.67-71 |
| issn | 0951-208X 1573-6784 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15724971 |
| source | Springer Nature - Complete Springer Journals |
| subjects | galactomannan immunoglobulin G ligands protein A separation techniques |
| title | The synthesis of soluble polymer-ligand complexes for affinity precipitation studies |
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