Application of polyaniline-Langmuir-Blodgett films as a glucose biosensor
Langmuir-Blodgett films of the polyemeraldine base form of polyaniline (PANI) have been successfully deposited on an indium tin oxide (ITO) coated glass surface. The enzyme glucose oxidase (GOX) was entrapped between the layers of the Langmuir-Blodgett (LB) film. The immobilized GOX was tested for r...
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| Veröffentlicht in: | Materials Science & Engineering C 1995, Vol.3 (3), p.159-163 |
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| container_title | Materials Science & Engineering C |
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| creator | Ramanathan, Kumaran Ram, M.K. Malhotra, B.D. Murthy, A.Surya N. |
| description | Langmuir-Blodgett films of the polyemeraldine base form of polyaniline (PANI) have been successfully deposited on an indium tin oxide (ITO) coated glass surface. The enzyme glucose oxidase (GOX) was entrapped between the layers of the Langmuir-Blodgett (LB) film. The immobilized GOX was tested for retention of its catalytic activity and its ability to perform like a reagentless glucose biosensor. The electroactivity of such PANI-LB-GOX electrodes was tested using cyclic voltammetry (CV). On the basis of the anodic peak current at 0.7 V with respect to Ag/AgCl, a calibration curve for a PANI-LB-GOX based glucose biosensor was obtained. |
| doi_str_mv | 10.1016/0928-4931(95)00113-1 |
| format | Article |
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The enzyme glucose oxidase (GOX) was entrapped between the layers of the Langmuir-Blodgett (LB) film. The immobilized GOX was tested for retention of its catalytic activity and its ability to perform like a reagentless glucose biosensor. The electroactivity of such PANI-LB-GOX electrodes was tested using cyclic voltammetry (CV). On the basis of the anodic peak current at 0.7 V with respect to Ag/AgCl, a calibration curve for a PANI-LB-GOX based glucose biosensor was obtained.</description><identifier>ISSN: 0928-4931</identifier><identifier>EISSN: 1873-0191</identifier><identifier>DOI: 10.1016/0928-4931(95)00113-1</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biosensors ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Glucose oxidase ; Immobilization ; Methods. Procedures. 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| identifier | ISSN: 0928-4931 |
| ispartof | Materials Science & Engineering C, 1995, Vol.3 (3), p.159-163 |
| issn | 0928-4931 1873-0191 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Biosensors Biotechnology Fundamental and applied biological sciences. Psychology Glucose oxidase Immobilization Methods. Procedures. Technologies Monolayer Multilayer Polyemeraldine base Pressure-area isotherm Various methods and equipments |
| title | Application of polyaniline-Langmuir-Blodgett films as a glucose biosensor |
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