Enzyme activity recovery from secondary fiber treated with cellulase and xylanase
One of the major problems with implementing biotechnical processes in the recycled paper industry, such as enzyme-enhanced deinking and enzymatic enhancement of pulp drainage properties, is the cost of commercial enzyme preparations. Thus, several factorial studies were performed to determine if enz...
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| Veröffentlicht in: | Journal of biotechnology 1996-01, Vol.45 (1), p.33-44 |
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| container_end_page | 44 |
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| container_issue | 1 |
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| container_title | Journal of biotechnology |
| container_volume | 45 |
| creator | Jackson, L.S. Joyce, T.W. Heitmann, J.A. Giesbrecht, F.G. |
| description | One of the major problems with implementing biotechnical processes in the recycled paper industry, such as enzyme-enhanced deinking and enzymatic enhancement of pulp drainage properties, is the cost of commercial enzyme preparations. Thus, several factorial studies were performed to determine if enzyme activity can be successfully removed from simulated recycled fiber (once-dried, bleached hardwood and softwood kraft fiber) treated with low concentrations (0.2% or 2.0% on oven-dry fiber) of cellulase or xylanase. Enzyme activity recovery was accomplished by washing treated fiber with dilute NaOH in combination with a low concentration of the nonionic surfactant Tween 80 under a variety of mild conditions. Various cellulase activities i.e., endoglucanase, exoglucanase, and filter paper, can be effectively recovered depending on the washing conditions, the cellulase charge, and the fiber type. Xylanase activity was effectively recovered from softwood, but not hardwood. The results suggest that enzyme activity recovery may be a possible means of decreasing the operating costs for biotechnical processes in the paper industry. |
| doi_str_mv | 10.1016/0168-1656(95)00137-9 |
| format | Article |
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Thus, several factorial studies were performed to determine if enzyme activity can be successfully removed from simulated recycled fiber (once-dried, bleached hardwood and softwood kraft fiber) treated with low concentrations (0.2% or 2.0% on oven-dry fiber) of cellulase or xylanase. Enzyme activity recovery was accomplished by washing treated fiber with dilute NaOH in combination with a low concentration of the nonionic surfactant Tween 80 under a variety of mild conditions. Various cellulase activities i.e., endoglucanase, exoglucanase, and filter paper, can be effectively recovered depending on the washing conditions, the cellulase charge, and the fiber type. Xylanase activity was effectively recovered from softwood, but not hardwood. The results suggest that enzyme activity recovery may be a possible means of decreasing the operating costs for biotechnical processes in the paper industry.</description><identifier>ISSN: 0168-1656</identifier><identifier>EISSN: 1873-4863</identifier><identifier>DOI: 10.1016/0168-1656(95)00137-9</identifier><identifier>CODEN: JBITD4</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biotechnology ; bleaching ; Cellulase ; cellulases ; enzyme activity ; Enzyme engineering ; Enzyme recovery ; Fundamental and applied biological sciences. Psychology ; hardwood ; kraft pulping ; Methods. Procedures. Technologies ; Miscellaneous ; nonionic surfactants ; O-glycoside hydrolases ; recovery ; Secondary fiber ; softwood ; tween 80 ; wood pulp ; Xylanase</subject><ispartof>Journal of biotechnology, 1996-01, Vol.45 (1), p.33-44</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-ee6cef0d5c9e26dbc786d4fc29ed90bc8f76af265736d77c6a8b681c2286fdf43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-1656(95)00137-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3007080$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Jackson, L.S.</creatorcontrib><creatorcontrib>Joyce, T.W.</creatorcontrib><creatorcontrib>Heitmann, J.A.</creatorcontrib><creatorcontrib>Giesbrecht, F.G.</creatorcontrib><title>Enzyme activity recovery from secondary fiber treated with cellulase and xylanase</title><title>Journal of biotechnology</title><description>One of the major problems with implementing biotechnical processes in the recycled paper industry, such as enzyme-enhanced deinking and enzymatic enhancement of pulp drainage properties, is the cost of commercial enzyme preparations. Thus, several factorial studies were performed to determine if enzyme activity can be successfully removed from simulated recycled fiber (once-dried, bleached hardwood and softwood kraft fiber) treated with low concentrations (0.2% or 2.0% on oven-dry fiber) of cellulase or xylanase. Enzyme activity recovery was accomplished by washing treated fiber with dilute NaOH in combination with a low concentration of the nonionic surfactant Tween 80 under a variety of mild conditions. Various cellulase activities i.e., endoglucanase, exoglucanase, and filter paper, can be effectively recovered depending on the washing conditions, the cellulase charge, and the fiber type. Xylanase activity was effectively recovered from softwood, but not hardwood. The results suggest that enzyme activity recovery may be a possible means of decreasing the operating costs for biotechnical processes in the paper industry.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>bleaching</subject><subject>Cellulase</subject><subject>cellulases</subject><subject>enzyme activity</subject><subject>Enzyme engineering</subject><subject>Enzyme recovery</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hardwood</subject><subject>kraft pulping</subject><subject>Methods. Procedures. Technologies</subject><subject>Miscellaneous</subject><subject>nonionic surfactants</subject><subject>O-glycoside hydrolases</subject><subject>recovery</subject><subject>Secondary fiber</subject><subject>softwood</subject><subject>tween 80</subject><subject>wood pulp</subject><subject>Xylanase</subject><issn>0168-1656</issn><issn>1873-4863</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqNkUtLAzEUhYMoWKv_QHAWIroYTWYmr40g4gsKItp1SJMbjUxnajKt1l9vxpYuxcXlcuA7h5sThA4JPieYsIs0IieMslNJzzAmJc_lFhoQwcu8EqzcRoMNsov2YnzHGFeSkgF6umm-l1PItOn8wnfLLIBpFxCWmQvtNItJNVb30k8gZF0A3YHNPn33lhmo63mtY3I3Nvta1rpJYh_tOF1HOFjvIRrf3rxc3-ejx7uH66tRbipKuxyAGXDYUiOhYHZiuGC2cqaQYCWeGOE4065glJfMcm6YFhMmiCkKwZx1VTlEJ6vcWWg_5hA7NfWxP0k30M6jIpQTTir6D5BJKQqcwGoFmtDGGMCpWfDT9HhFsOqLVn2Lqm9RSap-i1Yy2Y7X-ToaXbugG-PjxltizLHo049WmNOt0q8hIePnImVgQklVsDIRlysCUm0LD0FF46ExYH36lU7Z1v99yQ_nApwD</recordid><startdate>19960101</startdate><enddate>19960101</enddate><creator>Jackson, L.S.</creator><creator>Joyce, T.W.</creator><creator>Heitmann, J.A.</creator><creator>Giesbrecht, F.G.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>19960101</creationdate><title>Enzyme activity recovery from secondary fiber treated with cellulase and xylanase</title><author>Jackson, L.S. ; Joyce, T.W. ; Heitmann, J.A. ; Giesbrecht, F.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-ee6cef0d5c9e26dbc786d4fc29ed90bc8f76af265736d77c6a8b681c2286fdf43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>bleaching</topic><topic>Cellulase</topic><topic>cellulases</topic><topic>enzyme activity</topic><topic>Enzyme engineering</topic><topic>Enzyme recovery</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hardwood</topic><topic>kraft pulping</topic><topic>Methods. Procedures. Technologies</topic><topic>Miscellaneous</topic><topic>nonionic surfactants</topic><topic>O-glycoside hydrolases</topic><topic>recovery</topic><topic>Secondary fiber</topic><topic>softwood</topic><topic>tween 80</topic><topic>wood pulp</topic><topic>Xylanase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jackson, L.S.</creatorcontrib><creatorcontrib>Joyce, T.W.</creatorcontrib><creatorcontrib>Heitmann, J.A.</creatorcontrib><creatorcontrib>Giesbrecht, F.G.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jackson, L.S.</au><au>Joyce, T.W.</au><au>Heitmann, J.A.</au><au>Giesbrecht, F.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme activity recovery from secondary fiber treated with cellulase and xylanase</atitle><jtitle>Journal of biotechnology</jtitle><date>1996-01-01</date><risdate>1996</risdate><volume>45</volume><issue>1</issue><spage>33</spage><epage>44</epage><pages>33-44</pages><issn>0168-1656</issn><eissn>1873-4863</eissn><coden>JBITD4</coden><abstract>One of the major problems with implementing biotechnical processes in the recycled paper industry, such as enzyme-enhanced deinking and enzymatic enhancement of pulp drainage properties, is the cost of commercial enzyme preparations. Thus, several factorial studies were performed to determine if enzyme activity can be successfully removed from simulated recycled fiber (once-dried, bleached hardwood and softwood kraft fiber) treated with low concentrations (0.2% or 2.0% on oven-dry fiber) of cellulase or xylanase. Enzyme activity recovery was accomplished by washing treated fiber with dilute NaOH in combination with a low concentration of the nonionic surfactant Tween 80 under a variety of mild conditions. Various cellulase activities i.e., endoglucanase, exoglucanase, and filter paper, can be effectively recovered depending on the washing conditions, the cellulase charge, and the fiber type. Xylanase activity was effectively recovered from softwood, but not hardwood. The results suggest that enzyme activity recovery may be a possible means of decreasing the operating costs for biotechnical processes in the paper industry.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><doi>10.1016/0168-1656(95)00137-9</doi><tpages>12</tpages></addata></record> |
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| ispartof | Journal of biotechnology, 1996-01, Vol.45 (1), p.33-44 |
| issn | 0168-1656 1873-4863 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Biological and medical sciences Biotechnology bleaching Cellulase cellulases enzyme activity Enzyme engineering Enzyme recovery Fundamental and applied biological sciences. Psychology hardwood kraft pulping Methods. Procedures. Technologies Miscellaneous nonionic surfactants O-glycoside hydrolases recovery Secondary fiber softwood tween 80 wood pulp Xylanase |
| title | Enzyme activity recovery from secondary fiber treated with cellulase and xylanase |
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