Photolabelling of the prostaglandin E sub(2) receptor in cardiac sarcolemmal vesicles
A ( super(3)H)azidophenacyl ester of PGE sub(2) (( super(3)H)azido-PGE sub(2)) was synthesized and used to photoaffinity label the protein component of the high affinity PGE sub(2) binding site in cardiac sarcolemma membrane. Photolysis of the isolated cardiac sarcolemmal vesicles in the presence of...
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| Veröffentlicht in: | FEBS letters 1990-01, Vol.265 (1-2), p.117-120 |
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| creator | Michalak, M Wandler, EL Strynadka, K Lopaschuk, G L Njue, WM Liu, Hsing-Jang Olley, P M |
| description | A ( super(3)H)azidophenacyl ester of PGE sub(2) (( super(3)H)azido-PGE sub(2)) was synthesized and used to photoaffinity label the protein component of the high affinity PGE sub(2) binding site in cardiac sarcolemma membrane. Photolysis of the isolated cardiac sarcolemmal vesicles in the presence of ( super(3)H)azido-PGE sub(2) resulted in the covalent labelling of a protein component that migrated on sodium dodecyl sulfate-polyacrylamide gels with an apparent molecular weight of 100,000. Incorporation of the ( super(3)H)azido-PGE sub(2) did not occur in the absence of photolysis. The results indicate that the 100-kDa photoaffinity labelled ( super(3)H)azido-PGE sub(2) binding protein contains the binding site for PGE sub(2) in isolated cardiac sarcolemma membranes. |
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Photolysis of the isolated cardiac sarcolemmal vesicles in the presence of ( super(3)H)azido-PGE sub(2) resulted in the covalent labelling of a protein component that migrated on sodium dodecyl sulfate-polyacrylamide gels with an apparent molecular weight of 100,000. Incorporation of the ( super(3)H)azido-PGE sub(2) did not occur in the absence of photolysis. The results indicate that the 100-kDa photoaffinity labelled ( super(3)H)azido-PGE sub(2) binding protein contains the binding site for PGE sub(2) in isolated cardiac sarcolemma membranes.</description><identifier>ISSN: 0014-5793</identifier><language>eng</language><subject>membrane vesicles ; photoaffinity labelling ; sarcolemma</subject><ispartof>FEBS letters, 1990-01, Vol.265 (1-2), p.117-120</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Michalak, M</creatorcontrib><creatorcontrib>Wandler, EL</creatorcontrib><creatorcontrib>Strynadka, K</creatorcontrib><creatorcontrib>Lopaschuk, G L</creatorcontrib><creatorcontrib>Njue, WM</creatorcontrib><creatorcontrib>Liu, Hsing-Jang</creatorcontrib><creatorcontrib>Olley, P M</creatorcontrib><title>Photolabelling of the prostaglandin E sub(2) receptor in cardiac sarcolemmal vesicles</title><title>FEBS letters</title><description>A ( super(3)H)azidophenacyl ester of PGE sub(2) (( super(3)H)azido-PGE sub(2)) was synthesized and used to photoaffinity label the protein component of the high affinity PGE sub(2) binding site in cardiac sarcolemma membrane. Photolysis of the isolated cardiac sarcolemmal vesicles in the presence of ( super(3)H)azido-PGE sub(2) resulted in the covalent labelling of a protein component that migrated on sodium dodecyl sulfate-polyacrylamide gels with an apparent molecular weight of 100,000. Incorporation of the ( super(3)H)azido-PGE sub(2) did not occur in the absence of photolysis. The results indicate that the 100-kDa photoaffinity labelled ( super(3)H)azido-PGE sub(2) binding protein contains the binding site for PGE sub(2) in isolated cardiac sarcolemma membranes.</description><subject>membrane vesicles</subject><subject>photoaffinity labelling</subject><subject>sarcolemma</subject><issn>0014-5793</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><recordid>eNqNjb0OgkAQhK_QRPx5h62MFiScQJDaYCwttCbLscCZg8Pbw-eXwgewmsyXyTcLEUSRTMI0y-OVWDO_ormfZR6I572z3hqsyBg9tGAb8B3B6Cx7bA0OtR6gAJ6qw-kIjhSN3jqYoUJXa1TA6JQ11Pdo4EOslSHeimWDhmn3y43YX4vH5RbO3vdE7Mtes5ovcSA7cSnTLIplksd_D7_vqENS</recordid><startdate>19900101</startdate><enddate>19900101</enddate><creator>Michalak, M</creator><creator>Wandler, EL</creator><creator>Strynadka, K</creator><creator>Lopaschuk, G L</creator><creator>Njue, WM</creator><creator>Liu, Hsing-Jang</creator><creator>Olley, P M</creator><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope></search><sort><creationdate>19900101</creationdate><title>Photolabelling of the prostaglandin E sub(2) receptor in cardiac sarcolemmal vesicles</title><author>Michalak, M ; Wandler, EL ; Strynadka, K ; Lopaschuk, G L ; Njue, WM ; Liu, Hsing-Jang ; Olley, P M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_157031493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>membrane vesicles</topic><topic>photoaffinity labelling</topic><topic>sarcolemma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Michalak, M</creatorcontrib><creatorcontrib>Wandler, EL</creatorcontrib><creatorcontrib>Strynadka, K</creatorcontrib><creatorcontrib>Lopaschuk, G L</creatorcontrib><creatorcontrib>Njue, WM</creatorcontrib><creatorcontrib>Liu, Hsing-Jang</creatorcontrib><creatorcontrib>Olley, P M</creatorcontrib><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Michalak, M</au><au>Wandler, EL</au><au>Strynadka, K</au><au>Lopaschuk, G L</au><au>Njue, WM</au><au>Liu, Hsing-Jang</au><au>Olley, P M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photolabelling of the prostaglandin E sub(2) receptor in cardiac sarcolemmal vesicles</atitle><jtitle>FEBS letters</jtitle><date>1990-01-01</date><risdate>1990</risdate><volume>265</volume><issue>1-2</issue><spage>117</spage><epage>120</epage><pages>117-120</pages><issn>0014-5793</issn><abstract>A ( super(3)H)azidophenacyl ester of PGE sub(2) (( super(3)H)azido-PGE sub(2)) was synthesized and used to photoaffinity label the protein component of the high affinity PGE sub(2) binding site in cardiac sarcolemma membrane. Photolysis of the isolated cardiac sarcolemmal vesicles in the presence of ( super(3)H)azido-PGE sub(2) resulted in the covalent labelling of a protein component that migrated on sodium dodecyl sulfate-polyacrylamide gels with an apparent molecular weight of 100,000. Incorporation of the ( super(3)H)azido-PGE sub(2) did not occur in the absence of photolysis. The results indicate that the 100-kDa photoaffinity labelled ( super(3)H)azido-PGE sub(2) binding protein contains the binding site for PGE sub(2) in isolated cardiac sarcolemma membranes.</abstract></addata></record> |
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| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1990-01, Vol.265 (1-2), p.117-120 |
| issn | 0014-5793 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15703149 |
| source | Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | membrane vesicles photoaffinity labelling sarcolemma |
| title | Photolabelling of the prostaglandin E sub(2) receptor in cardiac sarcolemmal vesicles |
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