XMCM7, a Novel Member of the Xenopus MCM Family, Interacts with XMCM3 and Colocalizes with it throughout Replication
A minichromosome maintenance (MCM) protein complex has been implicated in restricting DNA replication to once per cell cycle in Xenopus egg extracts, based on the behavior of a single protein, XMCM3. Using a two-hybrid screen with XMCM3, we have identified a novel member of the MCM family in Xenopus...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1996-09, Vol.93 (19), p.10189-10194 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Romanowski, Piotr Madine, Mark A. Laskey, Ronald A. |
| description | A minichromosome maintenance (MCM) protein complex has been implicated in restricting DNA replication to once per cell cycle in Xenopus egg extracts, based on the behavior of a single protein, XMCM3. Using a two-hybrid screen with XMCM3, we have identified a novel member of the MCM family in Xenopus that is essential for DNA replication. The protein shows strong homology to Saccharomyces cerevisiae MCM7 (CDC47) and has thus been named XMCM7. XMCM7 is present in a multiprotein complex with other MCM proteins. It binds to chromatin and is displaced from chromatin by the act of replication. XMCM7 does not preferentially colocalize with sites of DNA replication but colocalizes with XMCM3 throughout replication. Immunodepletion of the MCM complex from Xenopus egg extract by anti-XMCM7 antibodies inhibits DNA replication of sperm and permeable HeLa G2 nuclei but not permeable HeLa G1 nuclei. Replication capacity of the Xenopus egg extract immunodepleted of the MCM complex by anti-XMCM7 antibody can be rescued by MCM proteins eluted from anti-XMCM3 antibody. We conclude that both proteins are present in the same complex in Xenopus egg extract throughout the cell cycle, that they remain together after binding to chromatin and during DNA replication, and that they perform similar functions. |
| doi_str_mv | 10.1073/pnas.93.19.10189 |
| format | Article |
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Using a two-hybrid screen with XMCM3, we have identified a novel member of the MCM family in Xenopus that is essential for DNA replication. The protein shows strong homology to Saccharomyces cerevisiae MCM7 (CDC47) and has thus been named XMCM7. XMCM7 is present in a multiprotein complex with other MCM proteins. It binds to chromatin and is displaced from chromatin by the act of replication. XMCM7 does not preferentially colocalize with sites of DNA replication but colocalizes with XMCM3 throughout replication. Immunodepletion of the MCM complex from Xenopus egg extract by anti-XMCM7 antibodies inhibits DNA replication of sperm and permeable HeLa G2 nuclei but not permeable HeLa G1 nuclei. Replication capacity of the Xenopus egg extract immunodepleted of the MCM complex by anti-XMCM7 antibody can be rescued by MCM proteins eluted from anti-XMCM3 antibody. We conclude that both proteins are present in the same complex in Xenopus egg extract throughout the cell cycle, that they remain together after binding to chromatin and during DNA replication, and that they perform similar functions.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.93.19.10189</identifier><identifier>PMID: 8816774</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Animals ; Antibodies ; Base Sequence ; Cell Cycle ; Cell Cycle Proteins - metabolism ; Cell Nucleus - metabolism ; Cells ; Cellular biology ; Chromatin ; Chromatin - physiology ; Deoxyribonucleic acid ; DNA ; DNA Primers ; DNA Replication ; DNA-Binding Proteins - metabolism ; Female ; Freshwater ; HeLa Cells ; Humans ; Interphase ; Male ; Minichromosome Maintenance Complex Component 3 ; Minichromosome Maintenance Complex Component 7 ; Molecular Sequence Data ; Nuclear Proteins - metabolism ; Oocytes - physiology ; Ova ; Proteins ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - physiology ; Saccharomyces cerevisiae Proteins ; Sequence Homology, Amino Acid ; Spermatozoa ; Spermatozoa - physiology ; Tissue Extracts ; Vertebrates ; Xenopus ; Xenopus Proteins ; Yeasts</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1996-09, Vol.93 (19), p.10189-10194</ispartof><rights>Copyright 1996 National Academy of Sciences</rights><rights>Copyright National Academy of Sciences Sep 17, 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c523t-c26c31f10222fb3aed55fb0de4f3c08cdf710e471930a3ab9b57fc2b4f7351683</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/93/19.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/40360$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/40360$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8816774$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Romanowski, Piotr</creatorcontrib><creatorcontrib>Madine, Mark A.</creatorcontrib><creatorcontrib>Laskey, Ronald A.</creatorcontrib><title>XMCM7, a Novel Member of the Xenopus MCM Family, Interacts with XMCM3 and Colocalizes with it throughout Replication</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A minichromosome maintenance (MCM) protein complex has been implicated in restricting DNA replication to once per cell cycle in Xenopus egg extracts, based on the behavior of a single protein, XMCM3. Using a two-hybrid screen with XMCM3, we have identified a novel member of the MCM family in Xenopus that is essential for DNA replication. The protein shows strong homology to Saccharomyces cerevisiae MCM7 (CDC47) and has thus been named XMCM7. XMCM7 is present in a multiprotein complex with other MCM proteins. It binds to chromatin and is displaced from chromatin by the act of replication. XMCM7 does not preferentially colocalize with sites of DNA replication but colocalizes with XMCM3 throughout replication. Immunodepletion of the MCM complex from Xenopus egg extract by anti-XMCM7 antibodies inhibits DNA replication of sperm and permeable HeLa G2 nuclei but not permeable HeLa G1 nuclei. Replication capacity of the Xenopus egg extract immunodepleted of the MCM complex by anti-XMCM7 antibody can be rescued by MCM proteins eluted from anti-XMCM3 antibody. We conclude that both proteins are present in the same complex in Xenopus egg extract throughout the cell cycle, that they remain together after binding to chromatin and during DNA replication, and that they perform similar functions.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Base Sequence</subject><subject>Cell Cycle</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Cell Nucleus - metabolism</subject><subject>Cells</subject><subject>Cellular biology</subject><subject>Chromatin</subject><subject>Chromatin - physiology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>DNA Replication</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Female</subject><subject>Freshwater</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Interphase</subject><subject>Male</subject><subject>Minichromosome Maintenance Complex Component 3</subject><subject>Minichromosome Maintenance Complex Component 7</subject><subject>Molecular Sequence Data</subject><subject>Nuclear Proteins - metabolism</subject><subject>Oocytes - physiology</subject><subject>Ova</subject><subject>Proteins</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - physiology</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Sequence Homology, Amino Acid</subject><subject>Spermatozoa</subject><subject>Spermatozoa - physiology</subject><subject>Tissue Extracts</subject><subject>Vertebrates</subject><subject>Xenopus</subject><subject>Xenopus Proteins</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFrFDEYhoModVu9iyAGD-KhsyaTyWQCXmSxWugqiEJvIZNJulmykzHJtNZfb8Ydl-pBTyG8z_PxJS8ATzBaYsTI66GXccnJEvN8xw2_BxYYcVzUFUf3wQKhkhVNVVYPwXGMW4QQpw06AkdNg2vGqgVIl-vVmp1CCT_6a-3gWu9aHaA3MG00vNS9H8YIMwPP5M6621N43icdpEoR3ti0gZNPoOw7uPLOK-nsDz1HNuUhwY9XGz8m-FkPziqZrO8fgQdGuqgfz-cJ-Hr27svqQ3Hx6f356u1FoWhJUqHKWhFsMCrL0rRE6o5S06JOV4Yo1KjOMIx0xTAnSBLZ8pYyo8q2MoxQXDfkBLzZzx3Gdqc7pfsUpBNDsDsZboWXVvyZ9HYjrvy1IA2hPOsvZz34b6OOSexsVNo52Ws_RsEaQhiv_g9impdhZAJf_AVu_Rj6_AeiRJjUjNI6Q2gPqeBjDNocFsZITK2LqXXBicBc_Go9K8_uPvQgzDXn_NWcT-bv9M4EYUbnkv6eMvr832gmnu6JbUw-HJAKkRqRn_mQyjs</recordid><startdate>19960917</startdate><enddate>19960917</enddate><creator>Romanowski, Piotr</creator><creator>Madine, Mark A.</creator><creator>Laskey, Ronald A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960917</creationdate><title>XMCM7, a Novel Member of the Xenopus MCM Family, Interacts with XMCM3 and Colocalizes with it throughout Replication</title><author>Romanowski, Piotr ; Madine, Mark A. ; Laskey, Ronald A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c523t-c26c31f10222fb3aed55fb0de4f3c08cdf710e471930a3ab9b57fc2b4f7351683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Base Sequence</topic><topic>Cell Cycle</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Cell Nucleus - metabolism</topic><topic>Cells</topic><topic>Cellular biology</topic><topic>Chromatin</topic><topic>Chromatin - physiology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Primers</topic><topic>DNA Replication</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Female</topic><topic>Freshwater</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Interphase</topic><topic>Male</topic><topic>Minichromosome Maintenance Complex Component 3</topic><topic>Minichromosome Maintenance Complex Component 7</topic><topic>Molecular Sequence Data</topic><topic>Nuclear Proteins - metabolism</topic><topic>Oocytes - physiology</topic><topic>Ova</topic><topic>Proteins</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - physiology</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Sequence Homology, Amino Acid</topic><topic>Spermatozoa</topic><topic>Spermatozoa - physiology</topic><topic>Tissue Extracts</topic><topic>Vertebrates</topic><topic>Xenopus</topic><topic>Xenopus Proteins</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Romanowski, Piotr</creatorcontrib><creatorcontrib>Madine, Mark A.</creatorcontrib><creatorcontrib>Laskey, Ronald A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Romanowski, Piotr</au><au>Madine, Mark A.</au><au>Laskey, Ronald A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>XMCM7, a Novel Member of the Xenopus MCM Family, Interacts with XMCM3 and Colocalizes with it throughout Replication</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1996-09-17</date><risdate>1996</risdate><volume>93</volume><issue>19</issue><spage>10189</spage><epage>10194</epage><pages>10189-10194</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>A minichromosome maintenance (MCM) protein complex has been implicated in restricting DNA replication to once per cell cycle in Xenopus egg extracts, based on the behavior of a single protein, XMCM3. Using a two-hybrid screen with XMCM3, we have identified a novel member of the MCM family in Xenopus that is essential for DNA replication. The protein shows strong homology to Saccharomyces cerevisiae MCM7 (CDC47) and has thus been named XMCM7. XMCM7 is present in a multiprotein complex with other MCM proteins. It binds to chromatin and is displaced from chromatin by the act of replication. XMCM7 does not preferentially colocalize with sites of DNA replication but colocalizes with XMCM3 throughout replication. Immunodepletion of the MCM complex from Xenopus egg extract by anti-XMCM7 antibodies inhibits DNA replication of sperm and permeable HeLa G2 nuclei but not permeable HeLa G1 nuclei. Replication capacity of the Xenopus egg extract immunodepleted of the MCM complex by anti-XMCM7 antibody can be rescued by MCM proteins eluted from anti-XMCM3 antibody. We conclude that both proteins are present in the same complex in Xenopus egg extract throughout the cell cycle, that they remain together after binding to chromatin and during DNA replication, and that they perform similar functions.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8816774</pmid><doi>10.1073/pnas.93.19.10189</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1996-09, Vol.93 (19), p.10189-10194 |
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| source | Jstor Complete Legacy; Open Access: PubMed Central; MEDLINE; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibodies Base Sequence Cell Cycle Cell Cycle Proteins - metabolism Cell Nucleus - metabolism Cells Cellular biology Chromatin Chromatin - physiology Deoxyribonucleic acid DNA DNA Primers DNA Replication DNA-Binding Proteins - metabolism Female Freshwater HeLa Cells Humans Interphase Male Minichromosome Maintenance Complex Component 3 Minichromosome Maintenance Complex Component 7 Molecular Sequence Data Nuclear Proteins - metabolism Oocytes - physiology Ova Proteins Saccharomyces cerevisiae Saccharomyces cerevisiae - physiology Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Spermatozoa Spermatozoa - physiology Tissue Extracts Vertebrates Xenopus Xenopus Proteins Yeasts |
| title | XMCM7, a Novel Member of the Xenopus MCM Family, Interacts with XMCM3 and Colocalizes with it throughout Replication |
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