In vitro responsiveness of Babesia bovis to imidocarb dipropionate and the selection of a drug-adapted line
A South African stock of Babesia bovis was successfully resuscitated from liquid nitrogen, and cultured in microaerophilous stationary phase. The in vitro susceptibility of the B. bovis stock to titrated concentrations of imidocarb dipropionate was observed and the 50% inhibitory concentration (IC)...
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| Veröffentlicht in: | Veterinary parasitology 1996-03, Vol.62 (1), p.35-41 |
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| creator | Rodriguez, R.I. Trees, A.J. |
| description | A South African stock of
Babesia bovis was successfully resuscitated from liquid nitrogen, and cultured in microaerophilous stationary phase. The in vitro susceptibility of the
B. bovis stock to titrated concentrations of imidocarb dipropionate was observed and the 50% inhibitory concentration (IC) was determinated (8.7 × 10
−7 g ml
−1). A drug-adapted line was developed by culture in the presence of sub-inhibitory concentrations of imidocarb dipropionate and it had an IC
50 eight times higher than that of its original stock (6.6 × 10
−6 g ml
−1). The drug-adapted line was cryopreserved and resuscitated from liquid nitrogen. Continuous culture of the non-drug adapted line through 15 subcultures did not change the IC
50 (8.3 × 10
−7 g ml
−1). |
| doi_str_mv | 10.1016/0304-4017(95)00850-0 |
| format | Article |
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Babesia bovis was successfully resuscitated from liquid nitrogen, and cultured in microaerophilous stationary phase. The in vitro susceptibility of the
B. bovis stock to titrated concentrations of imidocarb dipropionate was observed and the 50% inhibitory concentration (IC) was determinated (8.7 × 10
−7 g ml
−1). A drug-adapted line was developed by culture in the presence of sub-inhibitory concentrations of imidocarb dipropionate and it had an IC
50 eight times higher than that of its original stock (6.6 × 10
−6 g ml
−1). The drug-adapted line was cryopreserved and resuscitated from liquid nitrogen. Continuous culture of the non-drug adapted line through 15 subcultures did not change the IC
50 (8.3 × 10
−7 g ml
−1).</description><identifier>ISSN: 0304-4017</identifier><identifier>EISSN: 1873-2550</identifier><identifier>DOI: 10.1016/0304-4017(95)00850-0</identifier><identifier>PMID: 8638391</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antiprotozoal Agents - toxicity ; Babesia bovis ; Babesia bovis - drug effects ; Babesia bovis - growth & development ; Babesia bovis - isolation & purification ; Cattle ; Control methods-Protozoa ; Cryopreservation ; Drug Evaluation, Preclinical - methods ; Erythrocytes ; Female ; Imidocarb - analogs & derivatives ; Imidocarb - toxicity ; Imidocarb dipropionate ; In vitro culture ; Microbial Sensitivity Tests ; South Africa</subject><ispartof>Veterinary parasitology, 1996-03, Vol.62 (1), p.35-41</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-4a6e76963147d2eda750af8bb397df7e34ccc755e3a75fc088c2bea5b45adf2c3</citedby><cites>FETCH-LOGICAL-c357t-4a6e76963147d2eda750af8bb397df7e34ccc755e3a75fc088c2bea5b45adf2c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0304-4017(95)00850-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8638391$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rodriguez, R.I.</creatorcontrib><creatorcontrib>Trees, A.J.</creatorcontrib><title>In vitro responsiveness of Babesia bovis to imidocarb dipropionate and the selection of a drug-adapted line</title><title>Veterinary parasitology</title><addtitle>Vet Parasitol</addtitle><description>A South African stock of
Babesia bovis was successfully resuscitated from liquid nitrogen, and cultured in microaerophilous stationary phase. The in vitro susceptibility of the
B. bovis stock to titrated concentrations of imidocarb dipropionate was observed and the 50% inhibitory concentration (IC) was determinated (8.7 × 10
−7 g ml
−1). A drug-adapted line was developed by culture in the presence of sub-inhibitory concentrations of imidocarb dipropionate and it had an IC
50 eight times higher than that of its original stock (6.6 × 10
−6 g ml
−1). The drug-adapted line was cryopreserved and resuscitated from liquid nitrogen. Continuous culture of the non-drug adapted line through 15 subcultures did not change the IC
50 (8.3 × 10
−7 g ml
−1).</description><subject>Animals</subject><subject>Antiprotozoal Agents - toxicity</subject><subject>Babesia bovis</subject><subject>Babesia bovis - drug effects</subject><subject>Babesia bovis - growth & development</subject><subject>Babesia bovis - isolation & purification</subject><subject>Cattle</subject><subject>Control methods-Protozoa</subject><subject>Cryopreservation</subject><subject>Drug Evaluation, Preclinical - methods</subject><subject>Erythrocytes</subject><subject>Female</subject><subject>Imidocarb - analogs & derivatives</subject><subject>Imidocarb - toxicity</subject><subject>Imidocarb dipropionate</subject><subject>In vitro culture</subject><subject>Microbial Sensitivity Tests</subject><subject>South Africa</subject><issn>0304-4017</issn><issn>1873-2550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAUhYMo4zj6DxSyEl1Ub5qmSTeCDr5AcKPrkCa3Gu00NekM-O_tOINLVxfOOff1EXLM4IIBKy-BQ5EVwORZJc4BlIAMdsiUKcmzXAjYJdO_yD45SOkDAAoo5YRMVMkVr9iUfD52dOWHGGjE1Icu-RV2mBINDb0xNSZvaB1WPtEhUL_wLlgTa-p8H0PvQ2cGpKZzdHhHmrBFO4ziutlQF5dvmXGmH9DR1nd4SPYa0yY82tYZeb27fZk_ZE_P94_z66fMciGHrDAlyrIqOSuky9EZKcA0qq55JV0jkRfWWikE8tFpLChl8xqNqAthXJNbPiOnm7njjV9LTINe-GSxbU2HYZk0E6XKq1yNwWITtDGkFLHRffQLE781A71mrNcA9RqgroT-ZTxKM3Kynb-sF-j-mrZQR_9q4-P45Mpj1Ml67Cw6H0dA2gX__4Ifj3uM0g</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>Rodriguez, R.I.</creator><creator>Trees, A.J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>19960301</creationdate><title>In vitro responsiveness of Babesia bovis to imidocarb dipropionate and the selection of a drug-adapted line</title><author>Rodriguez, R.I. ; Trees, A.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-4a6e76963147d2eda750af8bb397df7e34ccc755e3a75fc088c2bea5b45adf2c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Antiprotozoal Agents - toxicity</topic><topic>Babesia bovis</topic><topic>Babesia bovis - drug effects</topic><topic>Babesia bovis - growth & development</topic><topic>Babesia bovis - isolation & purification</topic><topic>Cattle</topic><topic>Control methods-Protozoa</topic><topic>Cryopreservation</topic><topic>Drug Evaluation, Preclinical - methods</topic><topic>Erythrocytes</topic><topic>Female</topic><topic>Imidocarb - analogs & derivatives</topic><topic>Imidocarb - toxicity</topic><topic>Imidocarb dipropionate</topic><topic>In vitro culture</topic><topic>Microbial Sensitivity Tests</topic><topic>South Africa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodriguez, R.I.</creatorcontrib><creatorcontrib>Trees, A.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Veterinary parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodriguez, R.I.</au><au>Trees, A.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro responsiveness of Babesia bovis to imidocarb dipropionate and the selection of a drug-adapted line</atitle><jtitle>Veterinary parasitology</jtitle><addtitle>Vet Parasitol</addtitle><date>1996-03-01</date><risdate>1996</risdate><volume>62</volume><issue>1</issue><spage>35</spage><epage>41</epage><pages>35-41</pages><issn>0304-4017</issn><eissn>1873-2550</eissn><abstract>A South African stock of
Babesia bovis was successfully resuscitated from liquid nitrogen, and cultured in microaerophilous stationary phase. The in vitro susceptibility of the
B. bovis stock to titrated concentrations of imidocarb dipropionate was observed and the 50% inhibitory concentration (IC) was determinated (8.7 × 10
−7 g ml
−1). A drug-adapted line was developed by culture in the presence of sub-inhibitory concentrations of imidocarb dipropionate and it had an IC
50 eight times higher than that of its original stock (6.6 × 10
−6 g ml
−1). The drug-adapted line was cryopreserved and resuscitated from liquid nitrogen. Continuous culture of the non-drug adapted line through 15 subcultures did not change the IC
50 (8.3 × 10
−7 g ml
−1).</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8638391</pmid><doi>10.1016/0304-4017(95)00850-0</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-4017 |
| ispartof | Veterinary parasitology, 1996-03, Vol.62 (1), p.35-41 |
| issn | 0304-4017 1873-2550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15682928 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Antiprotozoal Agents - toxicity Babesia bovis Babesia bovis - drug effects Babesia bovis - growth & development Babesia bovis - isolation & purification Cattle Control methods-Protozoa Cryopreservation Drug Evaluation, Preclinical - methods Erythrocytes Female Imidocarb - analogs & derivatives Imidocarb - toxicity Imidocarb dipropionate In vitro culture Microbial Sensitivity Tests South Africa |
| title | In vitro responsiveness of Babesia bovis to imidocarb dipropionate and the selection of a drug-adapted line |
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