An enzyme-linked immunoassay for the levansucrase of Zymomonas mobilis using specific antibodies produced against the cloned enzyme

Levansucrase gene (levU) of Z. mobilis had been cloned and overexpressed in Escherichia coli. The levansucrase was purified and specific antibodies against it were raised in rabbits. Specificity of the antibodies was demonstrated by Western analysis. By using the antibodies, an efficient, enzyme-lin...

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Veröffentlicht in:	Biotechnology techniques 1996, Vol.10 (2), p.127-132
Hauptverfasser:	Kwak, Ju-Won, Seo, Young-Gyo, Song, Ki-Bang, Rhee, Sang-Ki
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Escherichia coli Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Others Various methods and equipments Zymomonas mobilis
Online-Zugang:	Volltext
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