An enzyme-linked immunoassay for the levansucrase of Zymomonas mobilis using specific antibodies produced against the cloned enzyme
Levansucrase gene (levU) of Z. mobilis had been cloned and overexpressed in Escherichia coli. The levansucrase was purified and specific antibodies against it were raised in rabbits. Specificity of the antibodies was demonstrated by Western analysis. By using the antibodies, an efficient, enzyme-lin...
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| Veröffentlicht in: | Biotechnology techniques 1996, Vol.10 (2), p.127-132 |
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| container_end_page | 132 |
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| container_issue | 2 |
| container_start_page | 127 |
| container_title | Biotechnology techniques |
| container_volume | 10 |
| creator | Kwak, Ju-Won Seo, Young-Gyo Song, Ki-Bang Rhee, Sang-Ki |
| description | Levansucrase gene (levU) of Z. mobilis had been cloned and overexpressed in Escherichia coli. The levansucrase was purified and specific antibodies against it were raised in rabbits. Specificity of the antibodies was demonstrated by Western analysis. By using the antibodies, an efficient, enzyme-linked immunosorbent assay (ELISA) amenable to immunochemical analysis of the levansucrase has been developed. The working range of levansucrase concentration in the developed ELISA was between 75 and 300 ng/ml. The assay was very sensitive to detect the levansucrase as little as 37 ng/ml. |
| doi_str_mv | 10.1007/BF00765195 |
| format | Article |
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The levansucrase was purified and specific antibodies against it were raised in rabbits. Specificity of the antibodies was demonstrated by Western analysis. By using the antibodies, an efficient, enzyme-linked immunosorbent assay (ELISA) amenable to immunochemical analysis of the levansucrase has been developed. The working range of levansucrase concentration in the developed ELISA was between 75 and 300 ng/ml. The assay was very sensitive to detect the levansucrase as little as 37 ng/ml.</description><identifier>ISSN: 0951-208X</identifier><identifier>EISSN: 1573-6784</identifier><identifier>DOI: 10.1007/BF00765195</identifier><identifier>CODEN: BTECE6</identifier><language>eng</language><publisher>London: Chapman and Hall</publisher><subject>Biological and medical sciences ; Biotechnology ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. 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The levansucrase was purified and specific antibodies against it were raised in rabbits. Specificity of the antibodies was demonstrated by Western analysis. By using the antibodies, an efficient, enzyme-linked immunosorbent assay (ELISA) amenable to immunochemical analysis of the levansucrase has been developed. The working range of levansucrase concentration in the developed ELISA was between 75 and 300 ng/ml. The assay was very sensitive to detect the levansucrase as little as 37 ng/ml.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. 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Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Others</topic><topic>Various methods and equipments</topic><topic>Zymomonas mobilis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kwak, Ju-Won</creatorcontrib><creatorcontrib>Seo, Young-Gyo</creatorcontrib><creatorcontrib>Song, Ki-Bang</creatorcontrib><creatorcontrib>Rhee, Sang-Ki</creatorcontrib><collection>Pascal-Francis</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biotechnology techniques</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kwak, Ju-Won</au><au>Seo, Young-Gyo</au><au>Song, Ki-Bang</au><au>Rhee, Sang-Ki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An enzyme-linked immunoassay for the levansucrase of Zymomonas mobilis using specific antibodies produced against the cloned enzyme</atitle><jtitle>Biotechnology techniques</jtitle><date>1996</date><risdate>1996</risdate><volume>10</volume><issue>2</issue><spage>127</spage><epage>132</epage><pages>127-132</pages><issn>0951-208X</issn><eissn>1573-6784</eissn><coden>BTECE6</coden><abstract>Levansucrase gene (levU) of Z. mobilis had been cloned and overexpressed in Escherichia coli. The levansucrase was purified and specific antibodies against it were raised in rabbits. Specificity of the antibodies was demonstrated by Western analysis. By using the antibodies, an efficient, enzyme-linked immunosorbent assay (ELISA) amenable to immunochemical analysis of the levansucrase has been developed. The working range of levansucrase concentration in the developed ELISA was between 75 and 300 ng/ml. The assay was very sensitive to detect the levansucrase as little as 37 ng/ml.</abstract><cop>London</cop><pub>Chapman and Hall</pub><doi>10.1007/BF00765195</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0951-208X |
| ispartof | Biotechnology techniques, 1996, Vol.10 (2), p.127-132 |
| issn | 0951-208X 1573-6784 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_15670776 |
| source | Alma/SFX Local Collection |
| subjects | Biological and medical sciences Biotechnology Escherichia coli Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Others Various methods and equipments Zymomonas mobilis |
| title | An enzyme-linked immunoassay for the levansucrase of Zymomonas mobilis using specific antibodies produced against the cloned enzyme |
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