Continuous stress promotes expression of VGF in melanotroph via suppression of dopamine
•VGF mRNA is induced in the intermediate lobe of pituitary under continuous stress (CS).•C-terminal peptide of VGF is induced in melanotroph under CS.•The CS-induced VGF expression is negatively regulated by dopamine. Prolonged exposure to stress elicits profound effects on homeostasis that may lead...
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| Veröffentlicht in: | Molecular and cellular endocrinology 2013-06, Vol.372 (1-2), p.49-56 |
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| creator | Tokizane, Kyohei Konishi, Hiroyuki Yasui, Masaya Ogawa, Tokiko Sasaki, Kazuki Minamino, Naoto Kiyama, Hiroshi |
| description | •VGF mRNA is induced in the intermediate lobe of pituitary under continuous stress (CS).•C-terminal peptide of VGF is induced in melanotroph under CS.•The CS-induced VGF expression is negatively regulated by dopamine.
Prolonged exposure to stress elicits profound effects on homeostasis that may lead to cryptogenic disorders such as chronic fatigue syndrome. To investigate the pathophysiology associated with the syndrome, we used a rat continuous stress (CS) model where the pituitary represents one of the most affected organs. Here we found that mRNA for VGF (non-acronymic), a member of the granin family, was induced specifically in the intermediate lobe (IL). This was matched by a concomitant increase at the peptide/protein level assessed by C-terminal antibody. Furthermore, the up-regulation of VGF was confirmed by immunohistochemistry in a subset of melanotrophs. VGF expression was altered in the IL of rats receivingthe dopamine D2 receptor agonist bromocriptine or the antagonist sulpiride. In vitro, dopamine dose-dependently decreased the mRNA levels in cultured melanotrophs. These findings suggest that VGF expression under CS is negatively regulated by dopaminergic neurons projecting from the hypothalamus. |
| doi_str_mv | 10.1016/j.mce.2013.03.012 |
| format | Article |
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Prolonged exposure to stress elicits profound effects on homeostasis that may lead to cryptogenic disorders such as chronic fatigue syndrome. To investigate the pathophysiology associated with the syndrome, we used a rat continuous stress (CS) model where the pituitary represents one of the most affected organs. Here we found that mRNA for VGF (non-acronymic), a member of the granin family, was induced specifically in the intermediate lobe (IL). This was matched by a concomitant increase at the peptide/protein level assessed by C-terminal antibody. Furthermore, the up-regulation of VGF was confirmed by immunohistochemistry in a subset of melanotrophs. VGF expression was altered in the IL of rats receivingthe dopamine D2 receptor agonist bromocriptine or the antagonist sulpiride. In vitro, dopamine dose-dependently decreased the mRNA levels in cultured melanotrophs. These findings suggest that VGF expression under CS is negatively regulated by dopaminergic neurons projecting from the hypothalamus.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/j.mce.2013.03.012</identifier><identifier>PMID: 23541636</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>agonists ; Animals ; antagonists ; antibodies ; bromocriptine ; Bromocriptine - pharmacology ; Cells, Cultured ; Cellular ; Chronic fatigue syndrome ; Chronic stress ; Disorders ; Dopamine ; Dopamine - physiology ; Dopamine Agonists - pharmacology ; Dopamine Antagonists - pharmacology ; Dopaminergic Neurons - metabolism ; Fatigue (materials) ; Gene Expression ; homeostasis ; Hypothalamus ; Hypothalamus - cytology ; Hypothalamus - metabolism ; immunohistochemistry ; Male ; Melanotrophs - metabolism ; messenger RNA ; neurons ; Neuropeptides - genetics ; Neuropeptides - metabolism ; pathophysiology ; Peptides ; Pituitary ; Pituitary Gland, Intermediate - cytology ; Pituitary Gland, Intermediate - drug effects ; Pituitary Gland, Intermediate - metabolism ; Primary Cell Culture ; Rats ; Rats, Sprague-Dawley ; Receiving ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Signal Transduction ; Stress, Physiological ; Stresses ; Sulpiride - pharmacology ; Transcriptional Activation</subject><ispartof>Molecular and cellular endocrinology, 2013-06, Vol.372 (1-2), p.49-56</ispartof><rights>2013 Elsevier Ireland Ltd</rights><rights>Copyright © 2013 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-bac3ddf941fec3e3ac429c728b43cc53ef24c37cd815f6f8540dee1a53f950b13</citedby><cites>FETCH-LOGICAL-c410t-bac3ddf941fec3e3ac429c728b43cc53ef24c37cd815f6f8540dee1a53f950b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mce.2013.03.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23541636$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tokizane, Kyohei</creatorcontrib><creatorcontrib>Konishi, Hiroyuki</creatorcontrib><creatorcontrib>Yasui, Masaya</creatorcontrib><creatorcontrib>Ogawa, Tokiko</creatorcontrib><creatorcontrib>Sasaki, Kazuki</creatorcontrib><creatorcontrib>Minamino, Naoto</creatorcontrib><creatorcontrib>Kiyama, Hiroshi</creatorcontrib><title>Continuous stress promotes expression of VGF in melanotroph via suppression of dopamine</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>•VGF mRNA is induced in the intermediate lobe of pituitary under continuous stress (CS).•C-terminal peptide of VGF is induced in melanotroph under CS.•The CS-induced VGF expression is negatively regulated by dopamine.
Prolonged exposure to stress elicits profound effects on homeostasis that may lead to cryptogenic disorders such as chronic fatigue syndrome. To investigate the pathophysiology associated with the syndrome, we used a rat continuous stress (CS) model where the pituitary represents one of the most affected organs. Here we found that mRNA for VGF (non-acronymic), a member of the granin family, was induced specifically in the intermediate lobe (IL). This was matched by a concomitant increase at the peptide/protein level assessed by C-terminal antibody. Furthermore, the up-regulation of VGF was confirmed by immunohistochemistry in a subset of melanotrophs. VGF expression was altered in the IL of rats receivingthe dopamine D2 receptor agonist bromocriptine or the antagonist sulpiride. In vitro, dopamine dose-dependently decreased the mRNA levels in cultured melanotrophs. These findings suggest that VGF expression under CS is negatively regulated by dopaminergic neurons projecting from the hypothalamus.</description><subject>agonists</subject><subject>Animals</subject><subject>antagonists</subject><subject>antibodies</subject><subject>bromocriptine</subject><subject>Bromocriptine - pharmacology</subject><subject>Cells, Cultured</subject><subject>Cellular</subject><subject>Chronic fatigue syndrome</subject><subject>Chronic stress</subject><subject>Disorders</subject><subject>Dopamine</subject><subject>Dopamine - physiology</subject><subject>Dopamine Agonists - pharmacology</subject><subject>Dopamine Antagonists - pharmacology</subject><subject>Dopaminergic Neurons - metabolism</subject><subject>Fatigue (materials)</subject><subject>Gene Expression</subject><subject>homeostasis</subject><subject>Hypothalamus</subject><subject>Hypothalamus - cytology</subject><subject>Hypothalamus - metabolism</subject><subject>immunohistochemistry</subject><subject>Male</subject><subject>Melanotrophs - metabolism</subject><subject>messenger RNA</subject><subject>neurons</subject><subject>Neuropeptides - genetics</subject><subject>Neuropeptides - metabolism</subject><subject>pathophysiology</subject><subject>Peptides</subject><subject>Pituitary</subject><subject>Pituitary Gland, Intermediate - cytology</subject><subject>Pituitary Gland, Intermediate - drug effects</subject><subject>Pituitary Gland, Intermediate - metabolism</subject><subject>Primary Cell Culture</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receiving</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Signal Transduction</subject><subject>Stress, Physiological</subject><subject>Stresses</subject><subject>Sulpiride - pharmacology</subject><subject>Transcriptional Activation</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMGKFDEQhoMo7rj6AF40Ry89ViWdTjeeZNidFRY86OoxZNKVNcN0p026F317M8zu4kmEgkD46uevj7HXCGsEbN7v14OjtQCUayiD4glbYatF1YLST9kKJMhKC9Bn7EXOewDQSrTP2ZmQqsZGNiv2fRPHOYxLXDLPc6Kc-ZTiEGfKnH5Nx48QRx49_7a95GHkAx3sGOcUpx_8Lliel-lvqo-THcJIL9kzbw-ZXt2_5-zm8uLr5qq6_rz9tPl4XbkaYa521sm-912NnpwkaV0tOqdFu6ulc0qSF7WT2vUtKt_4VtXQE6FV0ncKdijP2btTbmn9c6E8myFkR4dSkspNBlWjQYum_Q9U1h10SnRNQfGEuhRzTuTNlMJg02-DYI7qzd4U9eao3kAZFGXnzX38shuof9x4cF2AtyfA22jsbQrZ3HwpCQoASz1oC_HhRFAxdhcomewCjY76kMjNpo_hHwX-AAUSnk4</recordid><startdate>20130615</startdate><enddate>20130615</enddate><creator>Tokizane, Kyohei</creator><creator>Konishi, Hiroyuki</creator><creator>Yasui, Masaya</creator><creator>Ogawa, Tokiko</creator><creator>Sasaki, Kazuki</creator><creator>Minamino, Naoto</creator><creator>Kiyama, Hiroshi</creator><general>Elsevier Ireland Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>20130615</creationdate><title>Continuous stress promotes expression of VGF in melanotroph via suppression of dopamine</title><author>Tokizane, Kyohei ; Konishi, Hiroyuki ; Yasui, Masaya ; Ogawa, Tokiko ; Sasaki, Kazuki ; Minamino, Naoto ; Kiyama, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-bac3ddf941fec3e3ac429c728b43cc53ef24c37cd815f6f8540dee1a53f950b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>agonists</topic><topic>Animals</topic><topic>antagonists</topic><topic>antibodies</topic><topic>bromocriptine</topic><topic>Bromocriptine - pharmacology</topic><topic>Cells, Cultured</topic><topic>Cellular</topic><topic>Chronic fatigue syndrome</topic><topic>Chronic stress</topic><topic>Disorders</topic><topic>Dopamine</topic><topic>Dopamine - physiology</topic><topic>Dopamine Agonists - pharmacology</topic><topic>Dopamine Antagonists - pharmacology</topic><topic>Dopaminergic Neurons - metabolism</topic><topic>Fatigue (materials)</topic><topic>Gene Expression</topic><topic>homeostasis</topic><topic>Hypothalamus</topic><topic>Hypothalamus - cytology</topic><topic>Hypothalamus - metabolism</topic><topic>immunohistochemistry</topic><topic>Male</topic><topic>Melanotrophs - metabolism</topic><topic>messenger RNA</topic><topic>neurons</topic><topic>Neuropeptides - genetics</topic><topic>Neuropeptides - metabolism</topic><topic>pathophysiology</topic><topic>Peptides</topic><topic>Pituitary</topic><topic>Pituitary Gland, Intermediate - cytology</topic><topic>Pituitary Gland, Intermediate - drug effects</topic><topic>Pituitary Gland, Intermediate - metabolism</topic><topic>Primary Cell Culture</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receiving</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Signal Transduction</topic><topic>Stress, Physiological</topic><topic>Stresses</topic><topic>Sulpiride - pharmacology</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tokizane, Kyohei</creatorcontrib><creatorcontrib>Konishi, Hiroyuki</creatorcontrib><creatorcontrib>Yasui, Masaya</creatorcontrib><creatorcontrib>Ogawa, Tokiko</creatorcontrib><creatorcontrib>Sasaki, Kazuki</creatorcontrib><creatorcontrib>Minamino, Naoto</creatorcontrib><creatorcontrib>Kiyama, Hiroshi</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tokizane, Kyohei</au><au>Konishi, Hiroyuki</au><au>Yasui, Masaya</au><au>Ogawa, Tokiko</au><au>Sasaki, Kazuki</au><au>Minamino, Naoto</au><au>Kiyama, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Continuous stress promotes expression of VGF in melanotroph via suppression of dopamine</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>2013-06-15</date><risdate>2013</risdate><volume>372</volume><issue>1-2</issue><spage>49</spage><epage>56</epage><pages>49-56</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><abstract>•VGF mRNA is induced in the intermediate lobe of pituitary under continuous stress (CS).•C-terminal peptide of VGF is induced in melanotroph under CS.•The CS-induced VGF expression is negatively regulated by dopamine.
Prolonged exposure to stress elicits profound effects on homeostasis that may lead to cryptogenic disorders such as chronic fatigue syndrome. To investigate the pathophysiology associated with the syndrome, we used a rat continuous stress (CS) model where the pituitary represents one of the most affected organs. Here we found that mRNA for VGF (non-acronymic), a member of the granin family, was induced specifically in the intermediate lobe (IL). This was matched by a concomitant increase at the peptide/protein level assessed by C-terminal antibody. Furthermore, the up-regulation of VGF was confirmed by immunohistochemistry in a subset of melanotrophs. VGF expression was altered in the IL of rats receivingthe dopamine D2 receptor agonist bromocriptine or the antagonist sulpiride. In vitro, dopamine dose-dependently decreased the mRNA levels in cultured melanotrophs. These findings suggest that VGF expression under CS is negatively regulated by dopaminergic neurons projecting from the hypothalamus.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>23541636</pmid><doi>10.1016/j.mce.2013.03.012</doi><tpages>8</tpages></addata></record> |
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| subjects | agonists Animals antagonists antibodies bromocriptine Bromocriptine - pharmacology Cells, Cultured Cellular Chronic fatigue syndrome Chronic stress Disorders Dopamine Dopamine - physiology Dopamine Agonists - pharmacology Dopamine Antagonists - pharmacology Dopaminergic Neurons - metabolism Fatigue (materials) Gene Expression homeostasis Hypothalamus Hypothalamus - cytology Hypothalamus - metabolism immunohistochemistry Male Melanotrophs - metabolism messenger RNA neurons Neuropeptides - genetics Neuropeptides - metabolism pathophysiology Peptides Pituitary Pituitary Gland, Intermediate - cytology Pituitary Gland, Intermediate - drug effects Pituitary Gland, Intermediate - metabolism Primary Cell Culture Rats Rats, Sprague-Dawley Receiving RNA, Messenger - genetics RNA, Messenger - metabolism Signal Transduction Stress, Physiological Stresses Sulpiride - pharmacology Transcriptional Activation |
| title | Continuous stress promotes expression of VGF in melanotroph via suppression of dopamine |
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