Purification and serology of virions of impatiens necrotic spot tospovirus
Impatiens necrotic spot tospovirus (INSV) virions were purified using a procedure devised for tomato spotted wilt tospovirus (TSWV) from systemically infected Nicotiana benthamiana plants grown at 33 degree C day/26 degree C night and a photoperiod of 14 hours. With plants grown at 24/18 degree C pu...
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| Veröffentlicht in: | European journal of plant pathology 1996-07, Vol.102 (6), p.563-568 |
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| container_title | European journal of plant pathology |
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| creator | Roggero, P. (Istituto di Fitovirologia Applicata CNR, Torino (Italy)) Adam, G Milne, R.G Lisa, V |
| description | Impatiens necrotic spot tospovirus (INSV) virions were purified using a procedure devised for tomato spotted wilt tospovirus (TSWV) from systemically infected Nicotiana benthamiana plants grown at 33 degree C day/26 degree C night and a photoperiod of 14 hours. With plants grown at 24/18 degree C purification was unsuccessful. In SDS-PAGE the protein pattern of INSV was similar to that reported for TSWV, except the appearance of a single G2 protein band. A polyclonal antiserum, prepared against virions, reacted in Western blots with INSV nucleoprotein and glycoproteins but only with TSWV glycoproteins. In DAS ELISA the antiserum reacted with both INSV and TSWV infected plant sap and, after absorption with TSWV, only with INSV. In TAS ELISA the antiserum trapped both INSV and TSWV nucleoproteins and glycoproteins as detected by specific monoclonal antibodies, and, after absorption with TSWV, only the homologous proteins. This appears to be the first report of the purification of INSV virions and the production of an antiserum reacting with both nucleoprotein and glycoprotein antigens. |
| doi_str_mv | 10.1007/BF01877023 |
| format | Article |
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In TAS ELISA the antiserum trapped both INSV and TSWV nucleoproteins and glycoproteins as detected by specific monoclonal antibodies, and, after absorption with TSWV, only the homologous proteins. This appears to be the first report of the purification of INSV virions and the production of an antiserum reacting with both nucleoprotein and glycoprotein antigens.</description><identifier>ISSN: 0929-1873</identifier><identifier>EISSN: 1573-8469</identifier><identifier>DOI: 10.1007/BF01877023</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Biological and medical sciences ; BUNYAVIRIDAE ; FOTOPERIODISMO ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control ; IMMUNOLOGIE ; IMMUNOLOGY ; impatiens necrotic spot virus ; INMUNOLOGIA ; Microbiology ; NICOTIANA ; Nicotiana benthamiana ; PHOTOPERIODICITE ; PHOTOPERIODICITY ; Phytopathology. Animal pests. 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In DAS ELISA the antiserum reacted with both INSV and TSWV infected plant sap and, after absorption with TSWV, only with INSV. In TAS ELISA the antiserum trapped both INSV and TSWV nucleoproteins and glycoproteins as detected by specific monoclonal antibodies, and, after absorption with TSWV, only the homologous proteins. This appears to be the first report of the purification of INSV virions and the production of an antiserum reacting with both nucleoprotein and glycoprotein antigens.</description><subject>Biological and medical sciences</subject><subject>BUNYAVIRIDAE</subject><subject>FOTOPERIODISMO</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Techniques. Transmission, epidemiology, ecology. 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| subjects | Biological and medical sciences BUNYAVIRIDAE FOTOPERIODISMO Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control IMMUNOLOGIE IMMUNOLOGY impatiens necrotic spot virus INMUNOLOGIA Microbiology NICOTIANA Nicotiana benthamiana PHOTOPERIODICITE PHOTOPERIODICITY Phytopathology. Animal pests. Plant and forest protection Plant viruses and viroids PURIFICACION PURIFICATION Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Techniques used in virology TEMPERATURA TEMPERATURE tospovirus Virology |
| title | Purification and serology of virions of impatiens necrotic spot tospovirus |
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