Quantifying Mitotic Chromosome Dynamics and Positioning

The proper organization and segregation of chromosomes during cell division is essential to the preservation of genomic integrity. To understand the mechanisms that spatially control the arrangement and dynamics of mitotic chromosomes requires imaging assays to quantitatively resolve their positions...

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Veröffentlicht in:Journal of cellular physiology 2014-10, Vol.229 (10), p.1301-1305
Hauptverfasser: Bissonette, Samantha, Stumpff, Jason
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Stumpff, Jason
description The proper organization and segregation of chromosomes during cell division is essential to the preservation of genomic integrity. To understand the mechanisms that spatially control the arrangement and dynamics of mitotic chromosomes requires imaging assays to quantitatively resolve their positions and movements. Here, we will discuss analytical approaches to investigate the position‐dependent control of mitotic chromosomes in cultured cells. These methods can be used to dissect the specific contributions of mitotic proteins to the molecular control of chromosome dynamics. J. Cell. Physiol. 229: 1301–1305, 2014. © 2014 Wiley Periodicals, Inc.
doi_str_mv 10.1002/jcp.24634
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subjects Animals
Cell Cycle Proteins - metabolism
Cells, Cultured
Chromosome Segregation
Chromosomes
Chromosomes - metabolism
Chromosomes - ultrastructure
Humans
Kinetics
Kinetochores - metabolism
Mitosis
Molecular Imaging - methods
title Quantifying Mitotic Chromosome Dynamics and Positioning
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