Molecular cloning and differential expression analysis of a squalene synthase gene from Dioscorea zingiberensis, an important pharmaceutical plant

Diosgenin is a steroid derived from cholesterol in plants and used as a typical initial intermediate for synthesis of numerous steroidal drugs in the world. Commercially, this compound is extracted mainly from the rhizomes or tubers of some Dioscorea species. Squalene synthase (SQS: EC 2.5.1.21) cat...

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Veröffentlicht in:	Molecular biology reports 2014-09, Vol.41 (9), p.6097-6104
Hauptverfasser:	Ye, Yun, Wang, Runfa, Jin, Liang, Shen, Junhao, Li, Xiaotong, Yang, Ting, Zhou, Mengzhuo, Yang, Zhifan, Chen, Yongqin
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence amino acid sequences amino acids biosynthesis cholesterol Cloning, Molecular complementary DNA condensation Dioscorea Dioscorea - enzymology Dioscorea - genetics Dioscorea zingiberensis diosgenin drugs Escherichia coli Farnesyl-Diphosphate Farnesyltransferase - chemistry Farnesyl-Diphosphate Farnesyltransferase - genetics Farnesyl-Diphosphate Farnesyltransferase - metabolism gene expression regulation Gene Expression Regulation, Enzymologic genes isoelectric point leaves molecular cloning Molecular Sequence Data molecular weight NADP (coenzyme) open reading frames Organ Specificity phytosterols Plant Leaves - genetics Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Plant Stems - genetics Plants, Medicinal - enzymology Plants, Medicinal - genetics Polyisoprenyl Phosphates - metabolism polypeptides quantitative polymerase chain reaction Rhizome - genetics rhizomes sequence analysis Sesquiterpenes - metabolism squalene tubers
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