Programming biological operating systems: genome design, assembly and activation
This Perspective takes the reader through the important steps in bacterial genome assembly and activation and concludes with an outlook on how customized genomes may be achieved. The DNA technologies developed over the past 20 years for reading and writing the genetic code converged when the first s...
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| Veröffentlicht in: | Nature methods 2014-05, Vol.11 (5), p.521-526 |
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| creator | Gibson, Daniel G |
| description | This Perspective takes the reader through the important steps in bacterial genome assembly and activation and concludes with an outlook on how customized genomes may be achieved.
The DNA technologies developed over the past 20 years for reading and writing the genetic code converged when the first synthetic cell was created 4 years ago. An outcome of this work has been an extraordinary set of tools for synthesizing, assembling, engineering and transplanting whole bacterial genomes. Technical progress, options and applications for bacterial genome design, assembly and activation are discussed. |
| doi_str_mv | 10.1038/nmeth.2894 |
| format | Article |
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The DNA technologies developed over the past 20 years for reading and writing the genetic code converged when the first synthetic cell was created 4 years ago. An outcome of this work has been an extraordinary set of tools for synthesizing, assembling, engineering and transplanting whole bacterial genomes. 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The DNA technologies developed over the past 20 years for reading and writing the genetic code converged when the first synthetic cell was created 4 years ago. An outcome of this work has been an extraordinary set of tools for synthesizing, assembling, engineering and transplanting whole bacterial genomes. Technical progress, options and applications for bacterial genome design, assembly and activation are discussed.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>24781325</pmid><doi>10.1038/nmeth.2894</doi><tpages>6</tpages></addata></record> |
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| subjects | 631/1647/1511 631/553 Bioinformatics Biological Microscopy Biological systems Biological Techniques Biomedical Engineering/Biotechnology Biotechnology - methods Cell-Free System Cloning, Molecular Deoxyribonucleases - metabolism Deoxyribonucleic acid Design DNA DNA - metabolism Gene Expression Regulation Genetic aspects Genetic engineering Genetic Engineering - methods Genetic research Genetic Vectors Genome, Bacterial Genomics Life Sciences Mycoplasma - genetics perspective Proteomics Saccharomyces cerevisiae - genetics Synthetic biology Synthetic Biology - methods Systems Biology |
| title | Programming biological operating systems: genome design, assembly and activation |
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