Structural Stability and Unfolding Properties of Cutinases from Thermobifida fusca

A comparative analysis of the structural and functional aspects along with equilibrium unfolding of two homologous cutinases, Cut1 and Cut2, from Thermobifida fusca was carried out. The CD and fluorescence profile at different pH in the range of 6 to 9 showed no structural variations for both cutina...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2014-09, Vol.174 (2), p.803-819
Hauptverfasser:	Hegde, Krishnamoorthy, Dasu, Veeranki Venkata
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinobacteria - enzymology Amino acids Biochemistry Biological and medical sciences Biotechnology Carboxylic Ester Hydrolases - chemistry Carboxylic Ester Hydrolases - metabolism Chemistry Chemistry and Materials Science Circular Dichroism Comparative analysis Fluorescence Fundamental and applied biological sciences. Psychology Guanidine - chemistry Hydrogen-Ion Concentration Models, Molecular Protein Conformation Protein Unfolding Proteins Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Temperature effects Urea Urea - chemistry
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