Aquimixticola soesokkakensis gen. nov., sp. nov., a novel lipolytic alphaproteobacterium isolated from the junction between the ocean and a freshwater spring, and reclassification of Roseovarius marinus as Pacificibacter marinus comb. nov. and emended description of the genus Pacificibacter
A Gram-negative, aerobic, non-flagellated and rod-shaped or ovoid bacterial strain, designated DSSK2-3ᵀ, was isolated from the junction between the ocean and a freshwater spring at Jeju island, South Korea. Strain DSSK2-3ᵀ was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2....
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| Veröffentlicht in: | Antonie van Leeuwenhoek 2014-10, Vol.106 (4), p.647-655 |
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| description | A Gram-negative, aerobic, non-flagellated and rod-shaped or ovoid bacterial strain, designated DSSK2-3ᵀ, was isolated from the junction between the ocean and a freshwater spring at Jeju island, South Korea. Strain DSSK2-3ᵀ was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees based on 16S rRNA gene sequences revealed that strain DSSK2-3ᵀ joins the cluster comprising the type strains of Pacificibacter maritimus and Roseovarius marinus, with which it exhibited the highest sequence similarity values of 96.04 and 95.75 %, respectively. Sequence similarities to the type strains of other recognized species were |
| doi_str_mv | 10.1007/s10482-014-0235-z |
| format | Article |
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Strain DSSK2-3ᵀ was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees based on 16S rRNA gene sequences revealed that strain DSSK2-3ᵀ joins the cluster comprising the type strains of Pacificibacter maritimus and Roseovarius marinus, with which it exhibited the highest sequence similarity values of 96.04 and 95.75 %, respectively. Sequence similarities to the type strains of other recognized species were <95.74 %. Strain DSSK2-3ᵀ was found to contain Q-10 as the predominant ubiquinone and C₁₈:₁ ω7c as the major fatty acid. The polar lipid profile of strain DSSK2-3ᵀ was found to contain phosphatidylglycerol, an unidentified lipid and an unidentified aminolipid as major components, which distinguished the strain from those of phylogenetically related taxa. The DNA G+C content of strain DSSK2-3ᵀ was determined to be 60.8 mol%. On the basis of the phylogenetic data and the chemotaxonomic and other phenotypic properties, strain DSSK2-3ᵀ is considered to represent a new genus and species within the class Alphaproteobacteria, for which the name Aquimixticola soesokkakensis gen. nov., sp. nov. is proposed. The type strain of A. soesokkakensis is DSSK2-3ᵀ (=KCTC 42137ᵀ = CECT 8620ᵀ). In this study, it is also proposed that R. marinus should be reclassified as a member of the genus Pacificibacter and the description of the genus Pacificibacter is emended.</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1007/s10482-014-0235-z</identifier><identifier>PMID: 25052535</identifier><language>eng</language><publisher>Cham: Springer-Verlag</publisher><subject>Aerobiosis ; Bacterial Typing Techniques ; Base Composition ; Biomedical and Life Sciences ; chemotaxonomy ; Cluster Analysis ; Cytosol - chemistry ; DNA ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Fatty acids ; Fatty Acids - analysis ; Genotype & phenotype ; Gram-negative bacteria ; Hydrogen-Ion Concentration ; Life Sciences ; Lipids ; Medical Microbiology ; Microbiology ; Molecular Sequence Data ; new genus ; nucleotide sequences ; Oceans ; Original Paper ; Phospholipids - analysis ; Phylogeny ; Plant Sciences ; Quinones - analysis ; Republic of Korea ; Rhodobacteraceae - classification ; Rhodobacteraceae - genetics ; Rhodobacteraceae - isolation & purification ; Rhodobacteraceae - physiology ; ribosomal RNA ; RNA, Ribosomal, 16S - genetics ; Roseovarius ; Sequence Analysis, DNA ; sequence homology ; Sodium chloride ; Sodium Chloride - metabolism ; Soil Science & Conservation ; springs (water) ; taxonomic revisions ; Temperature ; Water Microbiology</subject><ispartof>Antonie van Leeuwenhoek, 2014-10, Vol.106 (4), p.647-655</ispartof><rights>Springer International Publishing Switzerland 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-e0439e3fbfbbf719f685eb84448d1e5624f96cc228b1191a05bcb6705c4f0ae73</citedby><cites>FETCH-LOGICAL-c462t-e0439e3fbfbbf719f685eb84448d1e5624f96cc228b1191a05bcb6705c4f0ae73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10482-014-0235-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10482-014-0235-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25052535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Sooyeon</creatorcontrib><creatorcontrib>Kang, Chul-Hyung</creatorcontrib><creatorcontrib>Park, Ji-Min</creatorcontrib><creatorcontrib>Yoon, Jung-Hoon</creatorcontrib><title>Aquimixticola soesokkakensis gen. nov., sp. nov., a novel lipolytic alphaproteobacterium isolated from the junction between the ocean and a freshwater spring, and reclassification of Roseovarius marinus as Pacificibacter marinus comb. nov. and emended description of the genus Pacificibacter</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie van Leeuwenhoek</addtitle><addtitle>Antonie Van Leeuwenhoek</addtitle><description>A Gram-negative, aerobic, non-flagellated and rod-shaped or ovoid bacterial strain, designated DSSK2-3ᵀ, was isolated from the junction between the ocean and a freshwater spring at Jeju island, South Korea. Strain DSSK2-3ᵀ was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees based on 16S rRNA gene sequences revealed that strain DSSK2-3ᵀ joins the cluster comprising the type strains of Pacificibacter maritimus and Roseovarius marinus, with which it exhibited the highest sequence similarity values of 96.04 and 95.75 %, respectively. Sequence similarities to the type strains of other recognized species were <95.74 %. Strain DSSK2-3ᵀ was found to contain Q-10 as the predominant ubiquinone and C₁₈:₁ ω7c as the major fatty acid. The polar lipid profile of strain DSSK2-3ᵀ was found to contain phosphatidylglycerol, an unidentified lipid and an unidentified aminolipid as major components, which distinguished the strain from those of phylogenetically related taxa. The DNA G+C content of strain DSSK2-3ᵀ was determined to be 60.8 mol%. On the basis of the phylogenetic data and the chemotaxonomic and other phenotypic properties, strain DSSK2-3ᵀ is considered to represent a new genus and species within the class Alphaproteobacteria, for which the name Aquimixticola soesokkakensis gen. nov., sp. nov. is proposed. The type strain of A. soesokkakensis is DSSK2-3ᵀ (=KCTC 42137ᵀ = CECT 8620ᵀ). In this study, it is also proposed that R. marinus should be reclassified as a member of the genus Pacificibacter and the description of the genus Pacificibacter is emended.</description><subject>Aerobiosis</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>Biomedical and Life Sciences</subject><subject>chemotaxonomy</subject><subject>Cluster Analysis</subject><subject>Cytosol - chemistry</subject><subject>DNA</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fatty acids</subject><subject>Fatty Acids - analysis</subject><subject>Genotype & phenotype</subject><subject>Gram-negative bacteria</subject><subject>Hydrogen-Ion Concentration</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>new genus</subject><subject>nucleotide sequences</subject><subject>Oceans</subject><subject>Original Paper</subject><subject>Phospholipids - analysis</subject><subject>Phylogeny</subject><subject>Plant Sciences</subject><subject>Quinones - analysis</subject><subject>Republic of Korea</subject><subject>Rhodobacteraceae - classification</subject><subject>Rhodobacteraceae - genetics</subject><subject>Rhodobacteraceae - isolation & purification</subject><subject>Rhodobacteraceae - physiology</subject><subject>ribosomal RNA</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Roseovarius</subject><subject>Sequence Analysis, DNA</subject><subject>sequence homology</subject><subject>Sodium chloride</subject><subject>Sodium Chloride - metabolism</subject><subject>Soil Science & Conservation</subject><subject>springs (water)</subject><subject>taxonomic revisions</subject><subject>Temperature</subject><subject>Water Microbiology</subject><issn>0003-6072</issn><issn>1572-9699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkk9v1DAQxQMC0aXwAbiApV44NIvtxE5yrCr-SZVAQM-R44x3vZvYW0_S0n56nE23Qj0gTpPYv_dmbL8kecPoklFafEBG85KnlOUp5ZlI754mCyYKnlayqp4lC0pplkpa8KPkJeIm_layLF4kR1xQwUUmFk9Ozq5G29vfg9W-UwQ9oN9u1RYcWiQrcEvi_PXylODu8KWmCh3p7M53t1FIVLdbq13wA_hG6QGCHXtiMRoO0BITfE-GNZDN6PRgvSMNDDcAbr_oNShHlGujrwmA65soCrFdsG51ut8IoDuFaI3Vaq_3hvzwCP5axU5I-lhcrArJd6UnzM5jPOxo3zfz-HtD6MG1cbIWUAe7O3hO48QTj49tXiXPjeoQXt_X4-Ty08df51_Si2-fv56fXaQ6l3xIgeZZBZlpTNOYglVGlgKaMs_zsmUgJM9NJbXmvGwYq5iiotGNLKjQuaEKiuw4eT_7xqu8GgGHureooeuUAz9izYSUZSZY9V8oY7zK5ISePEI3fgwuHmSiYjhKLvNIsZnSwSMGMHV8gHh9tzWj9RS2eg5bHcNWT2Gr76Lm7b3z2PTQPigO6YoAn4H5NSH81fofru9mkVG-Vqtgsb78ySNAKYuePM_-ALzB72E</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Park, Sooyeon</creator><creator>Kang, Chul-Hyung</creator><creator>Park, Ji-Min</creator><creator>Yoon, Jung-Hoon</creator><general>Springer-Verlag</general><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope></search><sort><creationdate>20141001</creationdate><title>Aquimixticola soesokkakensis gen. nov., sp. nov., a novel lipolytic alphaproteobacterium isolated from the junction between the ocean and a freshwater spring, and reclassification of Roseovarius marinus as Pacificibacter marinus comb. nov. and emended description of the genus Pacificibacter</title><author>Park, Sooyeon ; Kang, Chul-Hyung ; Park, Ji-Min ; Yoon, Jung-Hoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-e0439e3fbfbbf719f685eb84448d1e5624f96cc228b1191a05bcb6705c4f0ae73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Aerobiosis</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>Biomedical and Life Sciences</topic><topic>chemotaxonomy</topic><topic>Cluster Analysis</topic><topic>Cytosol - chemistry</topic><topic>DNA</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fatty acids</topic><topic>Fatty Acids - analysis</topic><topic>Genotype & phenotype</topic><topic>Gram-negative bacteria</topic><topic>Hydrogen-Ion Concentration</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>new genus</topic><topic>nucleotide sequences</topic><topic>Oceans</topic><topic>Original Paper</topic><topic>Phospholipids - analysis</topic><topic>Phylogeny</topic><topic>Plant Sciences</topic><topic>Quinones - analysis</topic><topic>Republic of Korea</topic><topic>Rhodobacteraceae - classification</topic><topic>Rhodobacteraceae - genetics</topic><topic>Rhodobacteraceae - isolation & purification</topic><topic>Rhodobacteraceae - physiology</topic><topic>ribosomal RNA</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Roseovarius</topic><topic>Sequence Analysis, DNA</topic><topic>sequence homology</topic><topic>Sodium chloride</topic><topic>Sodium Chloride - metabolism</topic><topic>Soil Science & Conservation</topic><topic>springs (water)</topic><topic>taxonomic revisions</topic><topic>Temperature</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Sooyeon</creatorcontrib><creatorcontrib>Kang, Chul-Hyung</creatorcontrib><creatorcontrib>Park, Ji-Min</creatorcontrib><creatorcontrib>Yoon, Jung-Hoon</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>Oceanic Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Antonie van Leeuwenhoek</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Sooyeon</au><au>Kang, Chul-Hyung</au><au>Park, Ji-Min</au><au>Yoon, Jung-Hoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aquimixticola soesokkakensis gen. nov., sp. nov., a novel lipolytic alphaproteobacterium isolated from the junction between the ocean and a freshwater spring, and reclassification of Roseovarius marinus as Pacificibacter marinus comb. nov. and emended description of the genus Pacificibacter</atitle><jtitle>Antonie van Leeuwenhoek</jtitle><stitle>Antonie van Leeuwenhoek</stitle><addtitle>Antonie Van Leeuwenhoek</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>106</volume><issue>4</issue><spage>647</spage><epage>655</epage><pages>647-655</pages><issn>0003-6072</issn><eissn>1572-9699</eissn><abstract>A Gram-negative, aerobic, non-flagellated and rod-shaped or ovoid bacterial strain, designated DSSK2-3ᵀ, was isolated from the junction between the ocean and a freshwater spring at Jeju island, South Korea. Strain DSSK2-3ᵀ was found to grow optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees based on 16S rRNA gene sequences revealed that strain DSSK2-3ᵀ joins the cluster comprising the type strains of Pacificibacter maritimus and Roseovarius marinus, with which it exhibited the highest sequence similarity values of 96.04 and 95.75 %, respectively. Sequence similarities to the type strains of other recognized species were <95.74 %. Strain DSSK2-3ᵀ was found to contain Q-10 as the predominant ubiquinone and C₁₈:₁ ω7c as the major fatty acid. The polar lipid profile of strain DSSK2-3ᵀ was found to contain phosphatidylglycerol, an unidentified lipid and an unidentified aminolipid as major components, which distinguished the strain from those of phylogenetically related taxa. The DNA G+C content of strain DSSK2-3ᵀ was determined to be 60.8 mol%. On the basis of the phylogenetic data and the chemotaxonomic and other phenotypic properties, strain DSSK2-3ᵀ is considered to represent a new genus and species within the class Alphaproteobacteria, for which the name Aquimixticola soesokkakensis gen. nov., sp. nov. is proposed. The type strain of A. soesokkakensis is DSSK2-3ᵀ (=KCTC 42137ᵀ = CECT 8620ᵀ). In this study, it is also proposed that R. marinus should be reclassified as a member of the genus Pacificibacter and the description of the genus Pacificibacter is emended.</abstract><cop>Cham</cop><pub>Springer-Verlag</pub><pmid>25052535</pmid><doi>10.1007/s10482-014-0235-z</doi><tpages>9</tpages></addata></record> |
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| ispartof | Antonie van Leeuwenhoek, 2014-10, Vol.106 (4), p.647-655 |
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| subjects | Aerobiosis Bacterial Typing Techniques Base Composition Biomedical and Life Sciences chemotaxonomy Cluster Analysis Cytosol - chemistry DNA DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Fatty acids Fatty Acids - analysis Genotype & phenotype Gram-negative bacteria Hydrogen-Ion Concentration Life Sciences Lipids Medical Microbiology Microbiology Molecular Sequence Data new genus nucleotide sequences Oceans Original Paper Phospholipids - analysis Phylogeny Plant Sciences Quinones - analysis Republic of Korea Rhodobacteraceae - classification Rhodobacteraceae - genetics Rhodobacteraceae - isolation & purification Rhodobacteraceae - physiology ribosomal RNA RNA, Ribosomal, 16S - genetics Roseovarius Sequence Analysis, DNA sequence homology Sodium chloride Sodium Chloride - metabolism Soil Science & Conservation springs (water) taxonomic revisions Temperature Water Microbiology |
| title | Aquimixticola soesokkakensis gen. nov., sp. nov., a novel lipolytic alphaproteobacterium isolated from the junction between the ocean and a freshwater spring, and reclassification of Roseovarius marinus as Pacificibacter marinus comb. nov. and emended description of the genus Pacificibacter |
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