The Establishment of Tetraploid Human Embryonic Cells Using Demecolcine

Diploid human embryonic cells were exposed to demecolcine for 3 d and then released from this exposure. At that time, the cell population was a mixture of diploid and tetraploid cells. Tetraploid cells were obtained through the cloning of this mixture of cells. The doubling time of the tetraploid ce...

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Veröffentlicht in:CYTOLOGIA 2013/12/25, Vol.78(4), pp.417-423
Hauptverfasser: Miyagoshi, Minoru, Fujikawa-Yamamoto, Kohzaburo, Yamagishi, Hiroko
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Sprache:eng
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