SLDP: a Novel Protein Related to Caleosin Is Associated with the Endosymbiotic Symbiodinium Lipid Droplets from Euphyllia glabrescens
Intracellular lipid droplets (LDs) have been proposed to play a key role in the mutualistic endosymbiosis between reef-building corals and the dinoflagellate endosymbiont Symbiodinium spp. This study investigates and identifies LD proteins in Symbiodinium from Euphyllia glabrescens. Discontinuous Pe...
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| Veröffentlicht in: | Marine biotechnology (New York, N.Y.) N.Y.), 2014-10, Vol.16 (5), p.560-571 |
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| creator | Pasaribu, Buntora Lin, I-Ping Tzen, Jason T. C. Jauh, Guang-Yuh Fan, Tung-Yung Ju, Yu-Min Cheng, Jing-O Chen, Chii-Shiarng Jiang, Pei-Luen |
| description | Intracellular lipid droplets (LDs) have been proposed to play a key role in the mutualistic endosymbiosis between reef-building corals and the dinoflagellate endosymbiont
Symbiodinium
spp. This study investigates and identifies LD proteins in
Symbiodinium
from
Euphyllia glabrescens.
Discontinuous Percoll gradient centrifugation was used to separate
Symbiodinium
cells from
E. glabrescens
tentacles. Furthermore, staining with a fluorescent probe, Nile red, indicated that lipids accumulated in that freshly isolated
Symbiodinium
cells and lipid analyses further showed polyunsaturated fatty acids (PUFA) was abundant. The stable LDs were purified from endosymbiotic
Symbiodinium
cells. The structural integrity of the
Symbiodinium
LDs was maintained via electronegative repulsion and steric hindrance possibly provided by their surface proteins. Protein extracts from the purified LDs revealed a major protein band with a molecular weight of 20 kDa, which was termed
Symbiodinium
lipid droplet protein (SLDP). Interestingly, immunological cross-recognition analysis revealed that SLDP was detected strongly by the anti-sesame and anti-cycad caleosin antibodies. It was suggested that the stable
Symbiodinium
LDs were sheltered by this unique structural protein and was suggested that SLDP might be homologous to caleosin to a certain extent. |
| doi_str_mv | 10.1007/s10126-014-9574-z |
| format | Article |
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Symbiodinium
spp. This study investigates and identifies LD proteins in
Symbiodinium
from
Euphyllia glabrescens.
Discontinuous Percoll gradient centrifugation was used to separate
Symbiodinium
cells from
E. glabrescens
tentacles. Furthermore, staining with a fluorescent probe, Nile red, indicated that lipids accumulated in that freshly isolated
Symbiodinium
cells and lipid analyses further showed polyunsaturated fatty acids (PUFA) was abundant. The stable LDs were purified from endosymbiotic
Symbiodinium
cells. The structural integrity of the
Symbiodinium
LDs was maintained via electronegative repulsion and steric hindrance possibly provided by their surface proteins. Protein extracts from the purified LDs revealed a major protein band with a molecular weight of 20 kDa, which was termed
Symbiodinium
lipid droplet protein (SLDP). Interestingly, immunological cross-recognition analysis revealed that SLDP was detected strongly by the anti-sesame and anti-cycad caleosin antibodies. It was suggested that the stable
Symbiodinium
LDs were sheltered by this unique structural protein and was suggested that SLDP might be homologous to caleosin to a certain extent.</description><identifier>ISSN: 1436-2228</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s10126-014-9574-z</identifier><identifier>PMID: 24820937</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Anthozoa - microbiology ; Biomedical and Life Sciences ; Biotechnology ; Blotting, Western ; Calcium-Binding Proteins - genetics ; Calcium-Binding Proteins - metabolism ; Centrifugation ; Coral reefs ; Dinoflagellida - genetics ; Dinoflagellida - metabolism ; Engineering ; Euphyllia ; Fatty acids ; Freshwater & Marine Ecology ; Gas Chromatography-Mass Spectrometry ; Image Processing, Computer-Assisted ; Life Sciences ; Lipid Droplets - metabolism ; Lipids ; Marine ; Marine biology ; Marine ecology ; Metabolism ; Microbiology ; Microorganisms ; Microscopy, Electron, Transmission ; Microscopy, Fluorescence ; Molecular weight ; Museums ; Original Article ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Polyunsaturated fatty acids ; Proteins ; Seeds ; Signal transduction ; Sterols ; Studies ; Symbiodinium ; Symbiosis ; Taiwan ; Zoology</subject><ispartof>Marine biotechnology (New York, N.Y.), 2014-10, Vol.16 (5), p.560-571</ispartof><rights>Springer Science+Business Media New York 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-cbcfdf59535e328195fb207b270ab7640b971c038d9e39e3686fa2bb2d6e55363</citedby><cites>FETCH-LOGICAL-c475t-cbcfdf59535e328195fb207b270ab7640b971c038d9e39e3686fa2bb2d6e55363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10126-014-9574-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10126-014-9574-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24820937$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pasaribu, Buntora</creatorcontrib><creatorcontrib>Lin, I-Ping</creatorcontrib><creatorcontrib>Tzen, Jason T. C.</creatorcontrib><creatorcontrib>Jauh, Guang-Yuh</creatorcontrib><creatorcontrib>Fan, Tung-Yung</creatorcontrib><creatorcontrib>Ju, Yu-Min</creatorcontrib><creatorcontrib>Cheng, Jing-O</creatorcontrib><creatorcontrib>Chen, Chii-Shiarng</creatorcontrib><creatorcontrib>Jiang, Pei-Luen</creatorcontrib><title>SLDP: a Novel Protein Related to Caleosin Is Associated with the Endosymbiotic Symbiodinium Lipid Droplets from Euphyllia glabrescens</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol</addtitle><addtitle>Mar Biotechnol (NY)</addtitle><description>Intracellular lipid droplets (LDs) have been proposed to play a key role in the mutualistic endosymbiosis between reef-building corals and the dinoflagellate endosymbiont
Symbiodinium
spp. This study investigates and identifies LD proteins in
Symbiodinium
from
Euphyllia glabrescens.
Discontinuous Percoll gradient centrifugation was used to separate
Symbiodinium
cells from
E. glabrescens
tentacles. Furthermore, staining with a fluorescent probe, Nile red, indicated that lipids accumulated in that freshly isolated
Symbiodinium
cells and lipid analyses further showed polyunsaturated fatty acids (PUFA) was abundant. The stable LDs were purified from endosymbiotic
Symbiodinium
cells. The structural integrity of the
Symbiodinium
LDs was maintained via electronegative repulsion and steric hindrance possibly provided by their surface proteins. Protein extracts from the purified LDs revealed a major protein band with a molecular weight of 20 kDa, which was termed
Symbiodinium
lipid droplet protein (SLDP). Interestingly, immunological cross-recognition analysis revealed that SLDP was detected strongly by the anti-sesame and anti-cycad caleosin antibodies. It was suggested that the stable
Symbiodinium
LDs were sheltered by this unique structural protein and was suggested that SLDP might be homologous to caleosin to a certain extent.</description><subject>Animals</subject><subject>Anthozoa - microbiology</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Blotting, Western</subject><subject>Calcium-Binding Proteins - genetics</subject><subject>Calcium-Binding Proteins - metabolism</subject><subject>Centrifugation</subject><subject>Coral reefs</subject><subject>Dinoflagellida - genetics</subject><subject>Dinoflagellida - metabolism</subject><subject>Engineering</subject><subject>Euphyllia</subject><subject>Fatty acids</subject><subject>Freshwater & Marine Ecology</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Image Processing, Computer-Assisted</subject><subject>Life Sciences</subject><subject>Lipid Droplets - metabolism</subject><subject>Lipids</subject><subject>Marine</subject><subject>Marine biology</subject><subject>Marine ecology</subject><subject>Metabolism</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Microscopy, Electron, Transmission</subject><subject>Microscopy, Fluorescence</subject><subject>Molecular weight</subject><subject>Museums</subject><subject>Original Article</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Polyunsaturated fatty acids</subject><subject>Proteins</subject><subject>Seeds</subject><subject>Signal transduction</subject><subject>Sterols</subject><subject>Studies</subject><subject>Symbiodinium</subject><subject>Symbiosis</subject><subject>Taiwan</subject><subject>Zoology</subject><issn>1436-2228</issn><issn>1436-2236</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkV1rFDEUhoMotlZ_gDcS8Mab0XxMkhnvynbVwqLF6nVIZs50UzKTMcko23v_t9luLSIIQkgOOc_75uNF6Dklrykh6k2ihDJZEVpXrVB1dfMAHdOay4oxLh_e16w5Qk9SuiZFozh5jI5Y3TDScnWMfl5uzi7eYoM_hu_g8UUMGdyEP4M3GXqcA14ZDyGVvfOET1MKnbvt_HB5i_MW8HrqQ9qN1oXsOnx5W_VucsuIN252PT6LYfaQEx5iGPF6mbc7753BV97YCKmDKT1FjwbjEzy7W0_Q13frL6sP1ebT-_PV6abqaiVy1dlu6AfRCi6As4a2YrCMKMsUMVbJmthW0Y7wpm-BlyEbORhmLeslCMElP0GvDr5zDN8WSFmPrlzAezNBWJKmQsqGtW3D_gMVrSyfWO9dX_6FXoclTuUhe6qRqi5zoeiB6mJIKcKg5-hGE3eaEr2PUx_i1CVOvY9T3xTNizvnxY7Q3yt-51cAdgBSaU1XEP84-p-uvwABGasr</recordid><startdate>20141001</startdate><enddate>20141001</enddate><creator>Pasaribu, Buntora</creator><creator>Lin, I-Ping</creator><creator>Tzen, Jason T. C.</creator><creator>Jauh, Guang-Yuh</creator><creator>Fan, Tung-Yung</creator><creator>Ju, Yu-Min</creator><creator>Cheng, Jing-O</creator><creator>Chen, Chii-Shiarng</creator><creator>Jiang, Pei-Luen</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7TN</scope><scope>7U9</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>L.F</scope><scope>L.G</scope><scope>L6V</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20141001</creationdate><title>SLDP: a Novel Protein Related to Caleosin Is Associated with the Endosymbiotic Symbiodinium Lipid Droplets from Euphyllia glabrescens</title><author>Pasaribu, Buntora ; Lin, I-Ping ; Tzen, Jason T. C. ; Jauh, Guang-Yuh ; Fan, Tung-Yung ; Ju, Yu-Min ; Cheng, Jing-O ; Chen, Chii-Shiarng ; Jiang, Pei-Luen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-cbcfdf59535e328195fb207b270ab7640b971c038d9e39e3686fa2bb2d6e55363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Anthozoa - microbiology</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Blotting, Western</topic><topic>Calcium-Binding Proteins - genetics</topic><topic>Calcium-Binding Proteins - metabolism</topic><topic>Centrifugation</topic><topic>Coral reefs</topic><topic>Dinoflagellida - genetics</topic><topic>Dinoflagellida - metabolism</topic><topic>Engineering</topic><topic>Euphyllia</topic><topic>Fatty acids</topic><topic>Freshwater & Marine Ecology</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Image Processing, Computer-Assisted</topic><topic>Life Sciences</topic><topic>Lipid Droplets - metabolism</topic><topic>Lipids</topic><topic>Marine</topic><topic>Marine biology</topic><topic>Marine ecology</topic><topic>Metabolism</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Microscopy, Electron, Transmission</topic><topic>Microscopy, Fluorescence</topic><topic>Molecular weight</topic><topic>Museums</topic><topic>Original Article</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Polyunsaturated fatty acids</topic><topic>Proteins</topic><topic>Seeds</topic><topic>Signal transduction</topic><topic>Sterols</topic><topic>Studies</topic><topic>Symbiodinium</topic><topic>Symbiosis</topic><topic>Taiwan</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pasaribu, Buntora</creatorcontrib><creatorcontrib>Lin, I-Ping</creatorcontrib><creatorcontrib>Tzen, Jason T. 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Academic</collection><jtitle>Marine biotechnology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pasaribu, Buntora</au><au>Lin, I-Ping</au><au>Tzen, Jason T. C.</au><au>Jauh, Guang-Yuh</au><au>Fan, Tung-Yung</au><au>Ju, Yu-Min</au><au>Cheng, Jing-O</au><au>Chen, Chii-Shiarng</au><au>Jiang, Pei-Luen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SLDP: a Novel Protein Related to Caleosin Is Associated with the Endosymbiotic Symbiodinium Lipid Droplets from Euphyllia glabrescens</atitle><jtitle>Marine biotechnology (New York, N.Y.)</jtitle><stitle>Mar Biotechnol</stitle><addtitle>Mar Biotechnol (NY)</addtitle><date>2014-10-01</date><risdate>2014</risdate><volume>16</volume><issue>5</issue><spage>560</spage><epage>571</epage><pages>560-571</pages><issn>1436-2228</issn><eissn>1436-2236</eissn><abstract>Intracellular lipid droplets (LDs) have been proposed to play a key role in the mutualistic endosymbiosis between reef-building corals and the dinoflagellate endosymbiont
Symbiodinium
spp. This study investigates and identifies LD proteins in
Symbiodinium
from
Euphyllia glabrescens.
Discontinuous Percoll gradient centrifugation was used to separate
Symbiodinium
cells from
E. glabrescens
tentacles. Furthermore, staining with a fluorescent probe, Nile red, indicated that lipids accumulated in that freshly isolated
Symbiodinium
cells and lipid analyses further showed polyunsaturated fatty acids (PUFA) was abundant. The stable LDs were purified from endosymbiotic
Symbiodinium
cells. The structural integrity of the
Symbiodinium
LDs was maintained via electronegative repulsion and steric hindrance possibly provided by their surface proteins. Protein extracts from the purified LDs revealed a major protein band with a molecular weight of 20 kDa, which was termed
Symbiodinium
lipid droplet protein (SLDP). Interestingly, immunological cross-recognition analysis revealed that SLDP was detected strongly by the anti-sesame and anti-cycad caleosin antibodies. It was suggested that the stable
Symbiodinium
LDs were sheltered by this unique structural protein and was suggested that SLDP might be homologous to caleosin to a certain extent.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>24820937</pmid><doi>10.1007/s10126-014-9574-z</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 1436-2228 |
| ispartof | Marine biotechnology (New York, N.Y.), 2014-10, Vol.16 (5), p.560-571 |
| issn | 1436-2228 1436-2236 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Anthozoa - microbiology Biomedical and Life Sciences Biotechnology Blotting, Western Calcium-Binding Proteins - genetics Calcium-Binding Proteins - metabolism Centrifugation Coral reefs Dinoflagellida - genetics Dinoflagellida - metabolism Engineering Euphyllia Fatty acids Freshwater & Marine Ecology Gas Chromatography-Mass Spectrometry Image Processing, Computer-Assisted Life Sciences Lipid Droplets - metabolism Lipids Marine Marine biology Marine ecology Metabolism Microbiology Microorganisms Microscopy, Electron, Transmission Microscopy, Fluorescence Molecular weight Museums Original Article Plant Proteins - genetics Plant Proteins - metabolism Polyunsaturated fatty acids Proteins Seeds Signal transduction Sterols Studies Symbiodinium Symbiosis Taiwan Zoology |
| title | SLDP: a Novel Protein Related to Caleosin Is Associated with the Endosymbiotic Symbiodinium Lipid Droplets from Euphyllia glabrescens |
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