Improving osteoblast functions and bone formation upon BMP-2 immobilization on titanium modified with heparin
•A Ti substrate can be successfully modified using heparin and BMP-2.•BMP-2/Hep-Ti induced osteoblast functions compared with Ti.•BMP-2/Hep-Ti improved new bone formation than Ti. The aim of this study was to develop bone morphogenetic protein-2 (BMP-2) immobilization on titanium (Ti) modified by he...
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| Veröffentlicht in: | Carbohydrate polymers 2014-12, Vol.114, p.123-132 |
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| creator | Kim, Sung Eun Kim, Chang-Seop Yun, Young-Pil Yang, Dae Hyeok Park, Kyeongsoon Kim, Se Eun Jeong, Chang-Mo Huh, Jung-Bo |
| description | •A Ti substrate can be successfully modified using heparin and BMP-2.•BMP-2/Hep-Ti induced osteoblast functions compared with Ti.•BMP-2/Hep-Ti improved new bone formation than Ti.
The aim of this study was to develop bone morphogenetic protein-2 (BMP-2) immobilization on titanium (Ti) modified by heparin for improving osteoblast function in vitro and bone formation in vivo. The Ti surface was first modified with heparin and then immobilized with BMP-2 (BMP-2/Hep-Ti). The Ti and modified Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle. In vitro studies demonstrated that osteoblasts cultured on BMP-2/Hep-Ti substrate increased ALP activity, calcium deposition, osteocalcin (OCN) and osteopontin (OPN) levels as compared to those cultured on Ti or BMP-2/Ti. In addition, intra-thread bone density and bone to implant contact ratio of BMP-2/Hep-Ti were significantly greater than those of Ti in the in vivo study. In conclusion, BMP-2 immobilized Ti modified heparin implants seemed to be a suitable delivery system for BMP-2 to improve osteoblast functions and new bone formation at the defect area around an implant. |
| doi_str_mv | 10.1016/j.carbpol.2014.08.005 |
| format | Article |
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The aim of this study was to develop bone morphogenetic protein-2 (BMP-2) immobilization on titanium (Ti) modified by heparin for improving osteoblast function in vitro and bone formation in vivo. The Ti surface was first modified with heparin and then immobilized with BMP-2 (BMP-2/Hep-Ti). The Ti and modified Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle. In vitro studies demonstrated that osteoblasts cultured on BMP-2/Hep-Ti substrate increased ALP activity, calcium deposition, osteocalcin (OCN) and osteopontin (OPN) levels as compared to those cultured on Ti or BMP-2/Ti. In addition, intra-thread bone density and bone to implant contact ratio of BMP-2/Hep-Ti were significantly greater than those of Ti in the in vivo study. In conclusion, BMP-2 immobilized Ti modified heparin implants seemed to be a suitable delivery system for BMP-2 to improve osteoblast functions and new bone formation at the defect area around an implant.</description><identifier>ISSN: 0144-8617</identifier><identifier>EISSN: 1879-1344</identifier><identifier>DOI: 10.1016/j.carbpol.2014.08.005</identifier><identifier>PMID: 25263872</identifier><identifier>CODEN: CAPOD8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Animals ; Applied sciences ; Biological and medical sciences ; Bone formation ; Bone Morphogenetic Protein 2 - metabolism ; Bone morphogenetic protein-2 (BMP-2) ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cell Line, Tumor ; Dogs ; Exact sciences and technology ; Heparin ; Heparin - metabolism ; Heparin - pharmacology ; Humans ; Immobilized Proteins - metabolism ; Immobilized Proteins - pharmacology ; Medical sciences ; Natural polymers ; Osteoblast function ; Osteoblasts - drug effects ; Osteoblasts - physiology ; Osteogenesis - drug effects ; Osteogenesis - physiology ; Photoelectron Spectroscopy - methods ; Physicochemistry of polymers ; Starch and polysaccharides ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Technology. Biomaterials. Equipments ; Titanium (Ti) ; Titanium - metabolism ; Titanium - pharmacology</subject><ispartof>Carbohydrate polymers, 2014-12, Vol.114, p.123-132</ispartof><rights>2014 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-1878b5373daa33da155f5c6c2dde399bea31f1f496f5ddefc505ddab8adaaf03</citedby><cites>FETCH-LOGICAL-c395t-1878b5373daa33da155f5c6c2dde399bea31f1f496f5ddefc505ddab8adaaf03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carbpol.2014.08.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=28855202$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25263872$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Sung Eun</creatorcontrib><creatorcontrib>Kim, Chang-Seop</creatorcontrib><creatorcontrib>Yun, Young-Pil</creatorcontrib><creatorcontrib>Yang, Dae Hyeok</creatorcontrib><creatorcontrib>Park, Kyeongsoon</creatorcontrib><creatorcontrib>Kim, Se Eun</creatorcontrib><creatorcontrib>Jeong, Chang-Mo</creatorcontrib><creatorcontrib>Huh, Jung-Bo</creatorcontrib><title>Improving osteoblast functions and bone formation upon BMP-2 immobilization on titanium modified with heparin</title><title>Carbohydrate polymers</title><addtitle>Carbohydr Polym</addtitle><description>•A Ti substrate can be successfully modified using heparin and BMP-2.•BMP-2/Hep-Ti induced osteoblast functions compared with Ti.•BMP-2/Hep-Ti improved new bone formation than Ti.
The aim of this study was to develop bone morphogenetic protein-2 (BMP-2) immobilization on titanium (Ti) modified by heparin for improving osteoblast function in vitro and bone formation in vivo. The Ti surface was first modified with heparin and then immobilized with BMP-2 (BMP-2/Hep-Ti). The Ti and modified Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle. In vitro studies demonstrated that osteoblasts cultured on BMP-2/Hep-Ti substrate increased ALP activity, calcium deposition, osteocalcin (OCN) and osteopontin (OPN) levels as compared to those cultured on Ti or BMP-2/Ti. In addition, intra-thread bone density and bone to implant contact ratio of BMP-2/Hep-Ti were significantly greater than those of Ti in the in vivo study. In conclusion, BMP-2 immobilized Ti modified heparin implants seemed to be a suitable delivery system for BMP-2 to improve osteoblast functions and new bone formation at the defect area around an implant.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Bone formation</subject><subject>Bone Morphogenetic Protein 2 - metabolism</subject><subject>Bone morphogenetic protein-2 (BMP-2)</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Line, Tumor</subject><subject>Dogs</subject><subject>Exact sciences and technology</subject><subject>Heparin</subject><subject>Heparin - metabolism</subject><subject>Heparin - pharmacology</subject><subject>Humans</subject><subject>Immobilized Proteins - metabolism</subject><subject>Immobilized Proteins - pharmacology</subject><subject>Medical sciences</subject><subject>Natural polymers</subject><subject>Osteoblast function</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - physiology</subject><subject>Osteogenesis - drug effects</subject><subject>Osteogenesis - physiology</subject><subject>Photoelectron Spectroscopy - methods</subject><subject>Physicochemistry of polymers</subject><subject>Starch and polysaccharides</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Technology. Biomaterials. Equipments</subject><subject>Titanium (Ti)</subject><subject>Titanium - metabolism</subject><subject>Titanium - pharmacology</subject><issn>0144-8617</issn><issn>1879-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi1ERZfCTwD5gtRLgh3HiXNCUBWoVASH3i3HH3RWsR1spwh-fb3aBY5Y1ow087xjz4vQK0paSujwdt9qleY1Lm1HaN8S0RLCn6AdFePUUNb3T9GuNvpGDHQ8R89z3pN6BkqeofOOdwMTY7dD_savKT5A-I5jLjbOi8oFuy3oAjFkrILBcwwWu5i8OtTwttbw4cu3psPgfZxhgd_HTr0FigqweeyjAQfW4J9Q7vG9XVWC8AKdObVk-_KUL9Ddx-u7q8_N7ddPN1fvbxvNJl6auoOYORuZUYrVQDl3XA-6M8ayaZqtYtRR10-D47XkNCc1q1moKnCEXaDL49i62o_N5iI9ZG2XRQUbtywpHwbRMTLSivIjqlPMOVkn1wRepV-SEnkwWu7lyWh5MFoSIavRVff69MQ2e2v-qv44W4E3J0BlrRaXVNCQ_3FCcN6RA_fuyNnqxwPYJLMGG7Q1kKwu0kT4z1ceAUw1oao</recordid><startdate>20141219</startdate><enddate>20141219</enddate><creator>Kim, Sung Eun</creator><creator>Kim, Chang-Seop</creator><creator>Yun, Young-Pil</creator><creator>Yang, Dae Hyeok</creator><creator>Park, Kyeongsoon</creator><creator>Kim, Se Eun</creator><creator>Jeong, Chang-Mo</creator><creator>Huh, Jung-Bo</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20141219</creationdate><title>Improving osteoblast functions and bone formation upon BMP-2 immobilization on titanium modified with heparin</title><author>Kim, Sung Eun ; Kim, Chang-Seop ; Yun, Young-Pil ; Yang, Dae Hyeok ; Park, Kyeongsoon ; Kim, Se Eun ; Jeong, Chang-Mo ; Huh, Jung-Bo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-1878b5373daa33da155f5c6c2dde399bea31f1f496f5ddefc505ddab8adaaf03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Bone formation</topic><topic>Bone Morphogenetic Protein 2 - metabolism</topic><topic>Bone morphogenetic protein-2 (BMP-2)</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Line, Tumor</topic><topic>Dogs</topic><topic>Exact sciences and technology</topic><topic>Heparin</topic><topic>Heparin - metabolism</topic><topic>Heparin - pharmacology</topic><topic>Humans</topic><topic>Immobilized Proteins - metabolism</topic><topic>Immobilized Proteins - pharmacology</topic><topic>Medical sciences</topic><topic>Natural polymers</topic><topic>Osteoblast function</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - physiology</topic><topic>Osteogenesis - drug effects</topic><topic>Osteogenesis - physiology</topic><topic>Photoelectron Spectroscopy - methods</topic><topic>Physicochemistry of polymers</topic><topic>Starch and polysaccharides</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Technology. Biomaterials. Equipments</topic><topic>Titanium (Ti)</topic><topic>Titanium - metabolism</topic><topic>Titanium - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Sung Eun</creatorcontrib><creatorcontrib>Kim, Chang-Seop</creatorcontrib><creatorcontrib>Yun, Young-Pil</creatorcontrib><creatorcontrib>Yang, Dae Hyeok</creatorcontrib><creatorcontrib>Park, Kyeongsoon</creatorcontrib><creatorcontrib>Kim, Se Eun</creatorcontrib><creatorcontrib>Jeong, Chang-Mo</creatorcontrib><creatorcontrib>Huh, Jung-Bo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Carbohydrate polymers</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Sung Eun</au><au>Kim, Chang-Seop</au><au>Yun, Young-Pil</au><au>Yang, Dae Hyeok</au><au>Park, Kyeongsoon</au><au>Kim, Se Eun</au><au>Jeong, Chang-Mo</au><au>Huh, Jung-Bo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improving osteoblast functions and bone formation upon BMP-2 immobilization on titanium modified with heparin</atitle><jtitle>Carbohydrate polymers</jtitle><addtitle>Carbohydr Polym</addtitle><date>2014-12-19</date><risdate>2014</risdate><volume>114</volume><spage>123</spage><epage>132</epage><pages>123-132</pages><issn>0144-8617</issn><eissn>1879-1344</eissn><coden>CAPOD8</coden><abstract>•A Ti substrate can be successfully modified using heparin and BMP-2.•BMP-2/Hep-Ti induced osteoblast functions compared with Ti.•BMP-2/Hep-Ti improved new bone formation than Ti.
The aim of this study was to develop bone morphogenetic protein-2 (BMP-2) immobilization on titanium (Ti) modified by heparin for improving osteoblast function in vitro and bone formation in vivo. The Ti surface was first modified with heparin and then immobilized with BMP-2 (BMP-2/Hep-Ti). The Ti and modified Ti were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle. In vitro studies demonstrated that osteoblasts cultured on BMP-2/Hep-Ti substrate increased ALP activity, calcium deposition, osteocalcin (OCN) and osteopontin (OPN) levels as compared to those cultured on Ti or BMP-2/Ti. In addition, intra-thread bone density and bone to implant contact ratio of BMP-2/Hep-Ti were significantly greater than those of Ti in the in vivo study. In conclusion, BMP-2 immobilized Ti modified heparin implants seemed to be a suitable delivery system for BMP-2 to improve osteoblast functions and new bone formation at the defect area around an implant.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>25263872</pmid><doi>10.1016/j.carbpol.2014.08.005</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Applied sciences Biological and medical sciences Bone formation Bone Morphogenetic Protein 2 - metabolism Bone morphogenetic protein-2 (BMP-2) Cell Differentiation - drug effects Cell Differentiation - physiology Cell Line, Tumor Dogs Exact sciences and technology Heparin Heparin - metabolism Heparin - pharmacology Humans Immobilized Proteins - metabolism Immobilized Proteins - pharmacology Medical sciences Natural polymers Osteoblast function Osteoblasts - drug effects Osteoblasts - physiology Osteogenesis - drug effects Osteogenesis - physiology Photoelectron Spectroscopy - methods Physicochemistry of polymers Starch and polysaccharides Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Technology. Biomaterials. Equipments Titanium (Ti) Titanium - metabolism Titanium - pharmacology |
| title | Improving osteoblast functions and bone formation upon BMP-2 immobilization on titanium modified with heparin |
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