Activation of autophagic pathways is related to growth inhibition and senescence in cutaneous squamous cell carcinoma
Cutaneous squamous cell carcinoma (SCC) is a very common resectable cancer; however, cutaneous SCC is highly resistant to chemotherapy if metastasis develops. Activating transcription factor 3 (ATF3) has been suggested as a marker of advanced or metastatic cutaneous SCC. Autophagy is one of the most...
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| Veröffentlicht in: | Experimental dermatology 2014-10, Vol.23 (10), p.718-724 |
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| creator | Choi, So Ra Chung, Bo Young Kim, Seong Who Kim, Chang Deok Yun, Woo Jin Lee, Mi Woo Choi, Jee Ho Chang, Sung Eun |
| description | Cutaneous squamous cell carcinoma (SCC) is a very common resectable cancer; however, cutaneous SCC is highly resistant to chemotherapy if metastasis develops. Activating transcription factor 3 (ATF3) has been suggested as a marker of advanced or metastatic cutaneous SCC. Autophagy is one of the most important mechanisms in cancer biology and commonly induced by in vitro serum starvation. To investigate the role of autophagy activation in cutaneous SCC, we activated autophagic pathways by serum starvation in SCC13 and ATF3‐overexpressing SCC13 (ATF3‐SCC13) cell lines. ATF3‐SCC13 cells demonstrated high proliferative capacity and low p53 and autophagy levels in comparison with control SCC13 cells under basal conditions. Intriguingly, autophagic stimulation via serum starvation resulted in growth inhibition and senescence in both cells, while ATF3‐SCC13 cells further demonstrated growth inhibition and senescence. Apoptosis was not significantly induced by autophagy activation. Taken together, autophagy activation may be a promising antitumor approach for advanced cutaneous SCC. |
| doi_str_mv | 10.1111/exd.12515 |
| format | Article |
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Activating transcription factor 3 (ATF3) has been suggested as a marker of advanced or metastatic cutaneous SCC. Autophagy is one of the most important mechanisms in cancer biology and commonly induced by in vitro serum starvation. To investigate the role of autophagy activation in cutaneous SCC, we activated autophagic pathways by serum starvation in SCC13 and ATF3‐overexpressing SCC13 (ATF3‐SCC13) cell lines. ATF3‐SCC13 cells demonstrated high proliferative capacity and low p53 and autophagy levels in comparison with control SCC13 cells under basal conditions. Intriguingly, autophagic stimulation via serum starvation resulted in growth inhibition and senescence in both cells, while ATF3‐SCC13 cells further demonstrated growth inhibition and senescence. Apoptosis was not significantly induced by autophagy activation. Taken together, autophagy activation may be a promising antitumor approach for advanced cutaneous SCC.</description><identifier>ISSN: 0906-6705</identifier><identifier>EISSN: 1600-0625</identifier><identifier>DOI: 10.1111/exd.12515</identifier><identifier>PMID: 25046976</identifier><language>eng</language><publisher>Denmark: Blackwell Publishing Ltd</publisher><subject>activating transcription factor 3 ; Activating Transcription Factor 3 - metabolism ; Aged ; Aged, 80 and over ; Apoptosis ; autophagy ; Autophagy - physiology ; Biomarkers, Tumor - metabolism ; Carcinoma, Squamous Cell - metabolism ; Carcinoma, Squamous Cell - pathology ; Carcinoma, Squamous Cell - secondary ; Cell Line, Tumor ; Cell Proliferation ; Cellular Senescence ; Culture Media, Serum-Free ; cutaneous squamous cell carcinoma ; Female ; growth inhibition ; Humans ; Male ; Middle Aged ; senescence ; Signal Transduction ; Skin Neoplasms - metabolism ; Skin Neoplasms - pathology ; Tumor Suppressor Protein p53 - metabolism</subject><ispartof>Experimental dermatology, 2014-10, Vol.23 (10), p.718-724</ispartof><rights>2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4295-5765223e926d4c43342e184c7f5cdc5572d1e22acb7480841161c323ae6a07513</citedby><cites>FETCH-LOGICAL-c4295-5765223e926d4c43342e184c7f5cdc5572d1e22acb7480841161c323ae6a07513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fexd.12515$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fexd.12515$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25046976$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choi, So Ra</creatorcontrib><creatorcontrib>Chung, Bo Young</creatorcontrib><creatorcontrib>Kim, Seong Who</creatorcontrib><creatorcontrib>Kim, Chang Deok</creatorcontrib><creatorcontrib>Yun, Woo Jin</creatorcontrib><creatorcontrib>Lee, Mi Woo</creatorcontrib><creatorcontrib>Choi, Jee Ho</creatorcontrib><creatorcontrib>Chang, Sung Eun</creatorcontrib><title>Activation of autophagic pathways is related to growth inhibition and senescence in cutaneous squamous cell carcinoma</title><title>Experimental dermatology</title><addtitle>Exp Dermatol</addtitle><description>Cutaneous squamous cell carcinoma (SCC) is a very common resectable cancer; however, cutaneous SCC is highly resistant to chemotherapy if metastasis develops. Activating transcription factor 3 (ATF3) has been suggested as a marker of advanced or metastatic cutaneous SCC. Autophagy is one of the most important mechanisms in cancer biology and commonly induced by in vitro serum starvation. To investigate the role of autophagy activation in cutaneous SCC, we activated autophagic pathways by serum starvation in SCC13 and ATF3‐overexpressing SCC13 (ATF3‐SCC13) cell lines. ATF3‐SCC13 cells demonstrated high proliferative capacity and low p53 and autophagy levels in comparison with control SCC13 cells under basal conditions. Intriguingly, autophagic stimulation via serum starvation resulted in growth inhibition and senescence in both cells, while ATF3‐SCC13 cells further demonstrated growth inhibition and senescence. Apoptosis was not significantly induced by autophagy activation. Taken together, autophagy activation may be a promising antitumor approach for advanced cutaneous SCC.</description><subject>activating transcription factor 3</subject><subject>Activating Transcription Factor 3 - metabolism</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Apoptosis</subject><subject>autophagy</subject><subject>Autophagy - physiology</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Carcinoma, Squamous Cell - metabolism</subject><subject>Carcinoma, Squamous Cell - pathology</subject><subject>Carcinoma, Squamous Cell - secondary</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Cellular Senescence</subject><subject>Culture Media, Serum-Free</subject><subject>cutaneous squamous cell carcinoma</subject><subject>Female</subject><subject>growth inhibition</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>senescence</subject><subject>Signal Transduction</subject><subject>Skin Neoplasms - metabolism</subject><subject>Skin Neoplasms - pathology</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><issn>0906-6705</issn><issn>1600-0625</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU9P3DAQxS1UBFvgwBeofGwPAdvxn-yRAgUk2nIAwc2adWZZt0m82E6X_fZkCXDrXGak-b2n0RtCDjk74kMd43N9xIXiaotMuGasYFqoT2TCpkwX2jC1Sz6n9IcxbkqjdsiuUEzqqdET0p-47P9B9qGjYU6hz2G5gEfv6BLyYgXrRH2iERvIWNMc6GMMq7ygvlv4mX-VQVfThB0mh53DYUNdn6HD0CeannpoN4PDpqEOovNdaGGfbM-hSXjw1vfI3Y_z29PL4vr3xdXpyXXhpJiqQhmthChxKnQtnSxLKZBX0pm5crVTyoiaoxDgZkZWrJKca-5KUQJqYEbxco98HX2XMTz1mLJtfdqcMp5nudJacqYqMaDfRtTFkFLEuV1G30JcW87sJmU7pGxfUx7YL2-2_azF-oN8j3UAjkdg5Rtc_9_Jnj-cvVsWo8KnjM8fCoh_rd48zd7_urD31cONlj_P7PfyBVmGlnQ</recordid><startdate>201410</startdate><enddate>201410</enddate><creator>Choi, So Ra</creator><creator>Chung, Bo Young</creator><creator>Kim, Seong Who</creator><creator>Kim, Chang Deok</creator><creator>Yun, Woo Jin</creator><creator>Lee, Mi Woo</creator><creator>Choi, Jee Ho</creator><creator>Chang, Sung Eun</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201410</creationdate><title>Activation of autophagic pathways is related to growth inhibition and senescence in cutaneous squamous cell carcinoma</title><author>Choi, So Ra ; Chung, Bo Young ; Kim, Seong Who ; Kim, Chang Deok ; Yun, Woo Jin ; Lee, Mi Woo ; Choi, Jee Ho ; Chang, Sung Eun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4295-5765223e926d4c43342e184c7f5cdc5572d1e22acb7480841161c323ae6a07513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>activating transcription factor 3</topic><topic>Activating Transcription Factor 3 - metabolism</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Apoptosis</topic><topic>autophagy</topic><topic>Autophagy - physiology</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Carcinoma, Squamous Cell - metabolism</topic><topic>Carcinoma, Squamous Cell - pathology</topic><topic>Carcinoma, Squamous Cell - secondary</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Cellular Senescence</topic><topic>Culture Media, Serum-Free</topic><topic>cutaneous squamous cell carcinoma</topic><topic>Female</topic><topic>growth inhibition</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>senescence</topic><topic>Signal Transduction</topic><topic>Skin Neoplasms - metabolism</topic><topic>Skin Neoplasms - pathology</topic><topic>Tumor Suppressor Protein p53 - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, So Ra</creatorcontrib><creatorcontrib>Chung, Bo Young</creatorcontrib><creatorcontrib>Kim, Seong Who</creatorcontrib><creatorcontrib>Kim, Chang Deok</creatorcontrib><creatorcontrib>Yun, Woo Jin</creatorcontrib><creatorcontrib>Lee, Mi Woo</creatorcontrib><creatorcontrib>Choi, Jee Ho</creatorcontrib><creatorcontrib>Chang, Sung Eun</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, So Ra</au><au>Chung, Bo Young</au><au>Kim, Seong Who</au><au>Kim, Chang Deok</au><au>Yun, Woo Jin</au><au>Lee, Mi Woo</au><au>Choi, Jee Ho</au><au>Chang, Sung Eun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation of autophagic pathways is related to growth inhibition and senescence in cutaneous squamous cell carcinoma</atitle><jtitle>Experimental dermatology</jtitle><addtitle>Exp Dermatol</addtitle><date>2014-10</date><risdate>2014</risdate><volume>23</volume><issue>10</issue><spage>718</spage><epage>724</epage><pages>718-724</pages><issn>0906-6705</issn><eissn>1600-0625</eissn><abstract>Cutaneous squamous cell carcinoma (SCC) is a very common resectable cancer; however, cutaneous SCC is highly resistant to chemotherapy if metastasis develops. Activating transcription factor 3 (ATF3) has been suggested as a marker of advanced or metastatic cutaneous SCC. Autophagy is one of the most important mechanisms in cancer biology and commonly induced by in vitro serum starvation. To investigate the role of autophagy activation in cutaneous SCC, we activated autophagic pathways by serum starvation in SCC13 and ATF3‐overexpressing SCC13 (ATF3‐SCC13) cell lines. ATF3‐SCC13 cells demonstrated high proliferative capacity and low p53 and autophagy levels in comparison with control SCC13 cells under basal conditions. Intriguingly, autophagic stimulation via serum starvation resulted in growth inhibition and senescence in both cells, while ATF3‐SCC13 cells further demonstrated growth inhibition and senescence. Apoptosis was not significantly induced by autophagy activation. Taken together, autophagy activation may be a promising antitumor approach for advanced cutaneous SCC.</abstract><cop>Denmark</cop><pub>Blackwell Publishing Ltd</pub><pmid>25046976</pmid><doi>10.1111/exd.12515</doi><tpages>7</tpages></addata></record> |
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| subjects | activating transcription factor 3 Activating Transcription Factor 3 - metabolism Aged Aged, 80 and over Apoptosis autophagy Autophagy - physiology Biomarkers, Tumor - metabolism Carcinoma, Squamous Cell - metabolism Carcinoma, Squamous Cell - pathology Carcinoma, Squamous Cell - secondary Cell Line, Tumor Cell Proliferation Cellular Senescence Culture Media, Serum-Free cutaneous squamous cell carcinoma Female growth inhibition Humans Male Middle Aged senescence Signal Transduction Skin Neoplasms - metabolism Skin Neoplasms - pathology Tumor Suppressor Protein p53 - metabolism |
| title | Activation of autophagic pathways is related to growth inhibition and senescence in cutaneous squamous cell carcinoma |
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