Effect of AST on age-associated changes of vocal folds in a rat model
Objectives/Hypothesis Reactive oxygen species (ROS) are associated with aging. Astaxanthin (AST) is a strong antioxidant and has been reported to prevent various ROS‐induced diseases. In the current study, we investigated the effect of AST on age‐associated histological and mRNA changes of vocal fol...
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| Veröffentlicht in: | The Laryngoscope 2014-10, Vol.124 (10), p.E411-E417 |
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| container_title | The Laryngoscope |
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| creator | Mizuta, Masanobu Hirano, Shigeru Hiwatashi, Nao Kobayashi, Toshiki Tateya, Ichiro Kanemaru, Shin-ichi Nakamura, Tatsuo Ito, Juichi |
| description | Objectives/Hypothesis
Reactive oxygen species (ROS) are associated with aging. Astaxanthin (AST) is a strong antioxidant and has been reported to prevent various ROS‐induced diseases. In the current study, we investigated the effect of AST on age‐associated histological and mRNA changes of vocal folds.
Study Design
Prospective animal experiment with control.
Methods
Six‐month‐old Sprague‐Dawley rats were fed on a normal powder diet with 0.01% (w/w) AST (aged AST‐treated group) or without AST (aged sham‐treated group). After 12 months of feeding, the larynges were harvested for histology, immunohistochemical detection of 4‐hydroxy‐2‐nonenal (4‐HNE), and quantitative real‐time polymerase chain reaction for basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF). Thirteen‐week‐old rats were used as a young control group (young group).
Results
The expression of 4‐HNE, an oxidative stress marker, significantly increased in the two aged groups compared with the young group. Histological examination showed that the deposition of hyaluronic acid in the lamina propria (LP) was significantly reduced in the aged sham‐treated group compared with the young group, but no significant difference was observed between the aged AST‐treated group and the young group. There were no significant differences in the mRNA expression of bFGF and HGF between the aged AST‐treated group and the young group, although the expression of these genes was significantly reduced in the aged sham‐treated group as compared with the young group.
Conclusions
These results suggest that AST has the potential to attenuate age‐associated changes of vocal folds.
Level of Evidence
N/A. Laryngoscope 124:E411–E417, 2014 |
| doi_str_mv | 10.1002/lary.24733 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1566407250</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3446029671</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5413-984493a86949cb71808fd4420be282f996b51997bd5e9c72d27cb6f12f2a07ad3</originalsourceid><addsrcrecordid>eNp90E9LwzAYBvAgipvTix9AAl5E6MzfpjkOmU4YCjpRTyFNk1ntmtl06r692aYePHgKL_m9Dy8PAIcY9TFC5KzSzbJPmKB0C3QxpzhhUvJt0I2fNMk4eeyAvRBeEMKCcrQLOhGnLA5dMBw6Z00LvYODuwn0NdRTm-gQvCl1awtonnU9tWEF3r3RFXS-KgIsI4SNbuHMF7baBztOV8EefL89cH8xnJyPkvHN5dX5YJwYzjBNZMaYpDpLJZMmFzhDmSsYIyi3JCNOyjTnWEqRF9xKI0hBhMlTh4kjGgld0B442eTOG_-2sKFVszIYW1W6tn4RFOZpypAgHEV6_Ie--EVTx-vWKtbEOYnqdKNM40NorFPzppzFPhVGalWuWpWr1uVGfPQduchntvilP21GgDfgo6zs8p8oNR7cPv2EJpudMrT283dHN68qFVRw9XB9qfCDQCxFI_VIvwBE5pAN</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1566153552</pqid></control><display><type>article</type><title>Effect of AST on age-associated changes of vocal folds in a rat model</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Mizuta, Masanobu ; Hirano, Shigeru ; Hiwatashi, Nao ; Kobayashi, Toshiki ; Tateya, Ichiro ; Kanemaru, Shin-ichi ; Nakamura, Tatsuo ; Ito, Juichi</creator><creatorcontrib>Mizuta, Masanobu ; Hirano, Shigeru ; Hiwatashi, Nao ; Kobayashi, Toshiki ; Tateya, Ichiro ; Kanemaru, Shin-ichi ; Nakamura, Tatsuo ; Ito, Juichi</creatorcontrib><description>Objectives/Hypothesis
Reactive oxygen species (ROS) are associated with aging. Astaxanthin (AST) is a strong antioxidant and has been reported to prevent various ROS‐induced diseases. In the current study, we investigated the effect of AST on age‐associated histological and mRNA changes of vocal folds.
Study Design
Prospective animal experiment with control.
Methods
Six‐month‐old Sprague‐Dawley rats were fed on a normal powder diet with 0.01% (w/w) AST (aged AST‐treated group) or without AST (aged sham‐treated group). After 12 months of feeding, the larynges were harvested for histology, immunohistochemical detection of 4‐hydroxy‐2‐nonenal (4‐HNE), and quantitative real‐time polymerase chain reaction for basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF). Thirteen‐week‐old rats were used as a young control group (young group).
Results
The expression of 4‐HNE, an oxidative stress marker, significantly increased in the two aged groups compared with the young group. Histological examination showed that the deposition of hyaluronic acid in the lamina propria (LP) was significantly reduced in the aged sham‐treated group compared with the young group, but no significant difference was observed between the aged AST‐treated group and the young group. There were no significant differences in the mRNA expression of bFGF and HGF between the aged AST‐treated group and the young group, although the expression of these genes was significantly reduced in the aged sham‐treated group as compared with the young group.
Conclusions
These results suggest that AST has the potential to attenuate age‐associated changes of vocal folds.
Level of Evidence
N/A. Laryngoscope 124:E411–E417, 2014</description><identifier>ISSN: 0023-852X</identifier><identifier>EISSN: 1531-4995</identifier><identifier>DOI: 10.1002/lary.24733</identifier><identifier>PMID: 24764173</identifier><language>eng</language><publisher>United States: Blackwell Publishing Ltd</publisher><subject>age-associated changes ; Aging - drug effects ; Aging - metabolism ; Aldehydes - metabolism ; Animals ; Antioxidants ; Antioxidants - pharmacology ; AST ; Chromatography, High Pressure Liquid ; Disease Models, Animal ; Fibrinolytic Agents ; Fibroblast Growth Factor 2 - biosynthesis ; Fibroblast Growth Factor 2 - genetics ; Follow-Up Studies ; Gene Expression Regulation ; Hepatocyte Growth Factor - biosynthesis ; Hepatocyte Growth Factor - genetics ; Immunohistochemistry ; Male ; Oxidative Stress ; Prospective Studies ; Rats ; Rats, Sprague-Dawley ; reactive oxygen species ; Reactive Oxygen Species - metabolism ; Real-Time Polymerase Chain Reaction ; RNA, Messenger - genetics ; Rodents ; Vocal Cord Dysfunction - genetics ; Vocal Cord Dysfunction - metabolism ; Vocal Cord Dysfunction - prevention & control ; Vocal Cords - drug effects ; Vocal Cords - metabolism ; Vocal Cords - pathology ; vocal folds ; Xanthophylls - pharmacology</subject><ispartof>The Laryngoscope, 2014-10, Vol.124 (10), p.E411-E417</ispartof><rights>2014 The American Laryngological, Rhinological and Otological Society, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5413-984493a86949cb71808fd4420be282f996b51997bd5e9c72d27cb6f12f2a07ad3</citedby><cites>FETCH-LOGICAL-c5413-984493a86949cb71808fd4420be282f996b51997bd5e9c72d27cb6f12f2a07ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Flary.24733$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Flary.24733$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24764173$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mizuta, Masanobu</creatorcontrib><creatorcontrib>Hirano, Shigeru</creatorcontrib><creatorcontrib>Hiwatashi, Nao</creatorcontrib><creatorcontrib>Kobayashi, Toshiki</creatorcontrib><creatorcontrib>Tateya, Ichiro</creatorcontrib><creatorcontrib>Kanemaru, Shin-ichi</creatorcontrib><creatorcontrib>Nakamura, Tatsuo</creatorcontrib><creatorcontrib>Ito, Juichi</creatorcontrib><title>Effect of AST on age-associated changes of vocal folds in a rat model</title><title>The Laryngoscope</title><addtitle>The Laryngoscope</addtitle><description>Objectives/Hypothesis
Reactive oxygen species (ROS) are associated with aging. Astaxanthin (AST) is a strong antioxidant and has been reported to prevent various ROS‐induced diseases. In the current study, we investigated the effect of AST on age‐associated histological and mRNA changes of vocal folds.
Study Design
Prospective animal experiment with control.
Methods
Six‐month‐old Sprague‐Dawley rats were fed on a normal powder diet with 0.01% (w/w) AST (aged AST‐treated group) or without AST (aged sham‐treated group). After 12 months of feeding, the larynges were harvested for histology, immunohistochemical detection of 4‐hydroxy‐2‐nonenal (4‐HNE), and quantitative real‐time polymerase chain reaction for basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF). Thirteen‐week‐old rats were used as a young control group (young group).
Results
The expression of 4‐HNE, an oxidative stress marker, significantly increased in the two aged groups compared with the young group. Histological examination showed that the deposition of hyaluronic acid in the lamina propria (LP) was significantly reduced in the aged sham‐treated group compared with the young group, but no significant difference was observed between the aged AST‐treated group and the young group. There were no significant differences in the mRNA expression of bFGF and HGF between the aged AST‐treated group and the young group, although the expression of these genes was significantly reduced in the aged sham‐treated group as compared with the young group.
Conclusions
These results suggest that AST has the potential to attenuate age‐associated changes of vocal folds.
Level of Evidence
N/A. Laryngoscope 124:E411–E417, 2014</description><subject>age-associated changes</subject><subject>Aging - drug effects</subject><subject>Aging - metabolism</subject><subject>Aldehydes - metabolism</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>AST</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Disease Models, Animal</subject><subject>Fibrinolytic Agents</subject><subject>Fibroblast Growth Factor 2 - biosynthesis</subject><subject>Fibroblast Growth Factor 2 - genetics</subject><subject>Follow-Up Studies</subject><subject>Gene Expression Regulation</subject><subject>Hepatocyte Growth Factor - biosynthesis</subject><subject>Hepatocyte Growth Factor - genetics</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Oxidative Stress</subject><subject>Prospective Studies</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Rodents</subject><subject>Vocal Cord Dysfunction - genetics</subject><subject>Vocal Cord Dysfunction - metabolism</subject><subject>Vocal Cord Dysfunction - prevention & control</subject><subject>Vocal Cords - drug effects</subject><subject>Vocal Cords - metabolism</subject><subject>Vocal Cords - pathology</subject><subject>vocal folds</subject><subject>Xanthophylls - pharmacology</subject><issn>0023-852X</issn><issn>1531-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90E9LwzAYBvAgipvTix9AAl5E6MzfpjkOmU4YCjpRTyFNk1ntmtl06r692aYePHgKL_m9Dy8PAIcY9TFC5KzSzbJPmKB0C3QxpzhhUvJt0I2fNMk4eeyAvRBeEMKCcrQLOhGnLA5dMBw6Z00LvYODuwn0NdRTm-gQvCl1awtonnU9tWEF3r3RFXS-KgIsI4SNbuHMF7baBztOV8EefL89cH8xnJyPkvHN5dX5YJwYzjBNZMaYpDpLJZMmFzhDmSsYIyi3JCNOyjTnWEqRF9xKI0hBhMlTh4kjGgld0B442eTOG_-2sKFVszIYW1W6tn4RFOZpypAgHEV6_Ie--EVTx-vWKtbEOYnqdKNM40NorFPzppzFPhVGalWuWpWr1uVGfPQduchntvilP21GgDfgo6zs8p8oNR7cPv2EJpudMrT283dHN68qFVRw9XB9qfCDQCxFI_VIvwBE5pAN</recordid><startdate>201410</startdate><enddate>201410</enddate><creator>Mizuta, Masanobu</creator><creator>Hirano, Shigeru</creator><creator>Hiwatashi, Nao</creator><creator>Kobayashi, Toshiki</creator><creator>Tateya, Ichiro</creator><creator>Kanemaru, Shin-ichi</creator><creator>Nakamura, Tatsuo</creator><creator>Ito, Juichi</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>201410</creationdate><title>Effect of AST on age-associated changes of vocal folds in a rat model</title><author>Mizuta, Masanobu ; Hirano, Shigeru ; Hiwatashi, Nao ; Kobayashi, Toshiki ; Tateya, Ichiro ; Kanemaru, Shin-ichi ; Nakamura, Tatsuo ; Ito, Juichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5413-984493a86949cb71808fd4420be282f996b51997bd5e9c72d27cb6f12f2a07ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>age-associated changes</topic><topic>Aging - drug effects</topic><topic>Aging - metabolism</topic><topic>Aldehydes - metabolism</topic><topic>Animals</topic><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>AST</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Disease Models, Animal</topic><topic>Fibrinolytic Agents</topic><topic>Fibroblast Growth Factor 2 - biosynthesis</topic><topic>Fibroblast Growth Factor 2 - genetics</topic><topic>Follow-Up Studies</topic><topic>Gene Expression Regulation</topic><topic>Hepatocyte Growth Factor - biosynthesis</topic><topic>Hepatocyte Growth Factor - genetics</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Oxidative Stress</topic><topic>Prospective Studies</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Rodents</topic><topic>Vocal Cord Dysfunction - genetics</topic><topic>Vocal Cord Dysfunction - metabolism</topic><topic>Vocal Cord Dysfunction - prevention & control</topic><topic>Vocal Cords - drug effects</topic><topic>Vocal Cords - metabolism</topic><topic>Vocal Cords - pathology</topic><topic>vocal folds</topic><topic>Xanthophylls - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mizuta, Masanobu</creatorcontrib><creatorcontrib>Hirano, Shigeru</creatorcontrib><creatorcontrib>Hiwatashi, Nao</creatorcontrib><creatorcontrib>Kobayashi, Toshiki</creatorcontrib><creatorcontrib>Tateya, Ichiro</creatorcontrib><creatorcontrib>Kanemaru, Shin-ichi</creatorcontrib><creatorcontrib>Nakamura, Tatsuo</creatorcontrib><creatorcontrib>Ito, Juichi</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>The Laryngoscope</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mizuta, Masanobu</au><au>Hirano, Shigeru</au><au>Hiwatashi, Nao</au><au>Kobayashi, Toshiki</au><au>Tateya, Ichiro</au><au>Kanemaru, Shin-ichi</au><au>Nakamura, Tatsuo</au><au>Ito, Juichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of AST on age-associated changes of vocal folds in a rat model</atitle><jtitle>The Laryngoscope</jtitle><addtitle>The Laryngoscope</addtitle><date>2014-10</date><risdate>2014</risdate><volume>124</volume><issue>10</issue><spage>E411</spage><epage>E417</epage><pages>E411-E417</pages><issn>0023-852X</issn><eissn>1531-4995</eissn><abstract>Objectives/Hypothesis
Reactive oxygen species (ROS) are associated with aging. Astaxanthin (AST) is a strong antioxidant and has been reported to prevent various ROS‐induced diseases. In the current study, we investigated the effect of AST on age‐associated histological and mRNA changes of vocal folds.
Study Design
Prospective animal experiment with control.
Methods
Six‐month‐old Sprague‐Dawley rats were fed on a normal powder diet with 0.01% (w/w) AST (aged AST‐treated group) or without AST (aged sham‐treated group). After 12 months of feeding, the larynges were harvested for histology, immunohistochemical detection of 4‐hydroxy‐2‐nonenal (4‐HNE), and quantitative real‐time polymerase chain reaction for basic fibroblast growth factor (bFGF) and hepatocyte growth factor (HGF). Thirteen‐week‐old rats were used as a young control group (young group).
Results
The expression of 4‐HNE, an oxidative stress marker, significantly increased in the two aged groups compared with the young group. Histological examination showed that the deposition of hyaluronic acid in the lamina propria (LP) was significantly reduced in the aged sham‐treated group compared with the young group, but no significant difference was observed between the aged AST‐treated group and the young group. There were no significant differences in the mRNA expression of bFGF and HGF between the aged AST‐treated group and the young group, although the expression of these genes was significantly reduced in the aged sham‐treated group as compared with the young group.
Conclusions
These results suggest that AST has the potential to attenuate age‐associated changes of vocal folds.
Level of Evidence
N/A. Laryngoscope 124:E411–E417, 2014</abstract><cop>United States</cop><pub>Blackwell Publishing Ltd</pub><pmid>24764173</pmid><doi>10.1002/lary.24733</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | age-associated changes Aging - drug effects Aging - metabolism Aldehydes - metabolism Animals Antioxidants Antioxidants - pharmacology AST Chromatography, High Pressure Liquid Disease Models, Animal Fibrinolytic Agents Fibroblast Growth Factor 2 - biosynthesis Fibroblast Growth Factor 2 - genetics Follow-Up Studies Gene Expression Regulation Hepatocyte Growth Factor - biosynthesis Hepatocyte Growth Factor - genetics Immunohistochemistry Male Oxidative Stress Prospective Studies Rats Rats, Sprague-Dawley reactive oxygen species Reactive Oxygen Species - metabolism Real-Time Polymerase Chain Reaction RNA, Messenger - genetics Rodents Vocal Cord Dysfunction - genetics Vocal Cord Dysfunction - metabolism Vocal Cord Dysfunction - prevention & control Vocal Cords - drug effects Vocal Cords - metabolism Vocal Cords - pathology vocal folds Xanthophylls - pharmacology |
| title | Effect of AST on age-associated changes of vocal folds in a rat model |
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